Acid phosphatases of <i>Esox lucius</i>: tissue distribution and partial characterization

Panara, Fausto

doi:10.1111/j.1095-8649.1997.tb01665.x

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…In support of this hypothesis, similar APN gene expression levels were detected in the intestine and RT of A. japonicus (Zhao et al, 2014). The results also infer a comparable efficiency of both organs on lipid digestion and on the intracellular digestion of proteins and phosphorylated nutrients (Panara, 1997;Sire & Vernier, 1992). Future studies are necessary to elucidate the role of enzymes in the RT digestive function and to evaluate the possible activity of proteases and other digestive enzymes in this organ.…”

Section: Discussionsupporting

confidence: 65%

Novel findings in sea cucumber's digestive capacities: Enzymatic activities in the respiratory tree, implications for aquaculture

Martínez-Milián

Olvera‐Novoa

Toledo-Cuevas

2021

J World Aquaculture Soc

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There is a demand for proper feed and feeding strategies in the growing sea cucumber aquaculture. The digestive system of holothurians seems to increase nutrient assimilation through the respiratory tree (RT), but digestive functioning in this organ has not been investigated. This study evaluated the enzymatic activity of the cytosolic leucine alanine peptidase (leu-ala) and acid phosphatase, the membranelinked aminopeptidase-N (APN), the bile salt-activated lipase, laminarinase (LAM), and β-glycosidase (BGL), in the RT and segments of the digestive tract (DT; foregut, midgut, and hindgut) of adult Isostichopus badionotus. All enzymes, except LAM and BGL, exhibited comparable activity levels in the RT and the DT segments, but APN and BGL activities were significantly higher in the whole DT compared with those in RT, with high activity of leu-ala in both organs.These results indicate a relevant role of the RT as an accessory organ in sea cucumber digestive physiology. Consequently, I. badionotus seems to be able to digest feeds from the water column through the RT, maximizing the use of available nutrients by luminal, intracellular, and apical membrane-linked digestive modes. These findings expand the knowledge of sea cucumber digestive mechanisms, with

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Section: Discussionsupporting

confidence: 65%

Novel findings in sea cucumber's digestive capacities: Enzymatic activities in the respiratory tree, implications for aquaculture

Martínez-Milián

Olvera‐Novoa

Toledo-Cuevas

2021

J World Aquaculture Soc

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“…AcP was observed in teleost fish testis and efferent ducts. Panara (1997) found 3 forms of AcP in pike (Esox lucius) testis. The molecular weight of one form (20 kDa) was similar to 2 nd fraction described in this manuscript.…”

Section: Discussionmentioning

confidence: 98%

Characteristics of acid phosphatase from Russian sturgeon (Acipenser gueldenstaedtii) spermatozoa

Sarosiek¹,

Wysocka²,

Wysocki³

et al. 2006

J Appl Ichthyol

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This paper provides data concerning the biochemical characteristics of acid phosphatase (AcP) from Russian sturgeon (Acipenser gueldenstaedti) spermatozoa. The extraction was performed in 0.85% NaCl with 0.1% Triton X-100, which stabilized the enzyme. AcP was partially purified by gel filtration. We found two different forms of AcP, i.e. fraction I and fraction II, with molecular weights of 39 kDa and 19 kDa respectively, determined during gel filtration and confirmed electrophoretically. The activity of AcP was different and related to the substrates p-nitrophenylphosphate (pNPP) or β-glycerophosphate (β-GP). It was about 20-fold higher with pNPP as a substrate. Ammonium molybdate was an inhibitor of the 1 st and 2 nd fraction of AcP, but L-tartaric acid was an inhibitor of fraction II only.

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“…Basic studies on AP in fish tissue have been performed by Panara (1997) in the northern pike, Esox lucius. Structure and function, as well as the amount, of this enzyme are similar to mammals.…”

Section: Ma-ap Correlation With Organochlorinesmentioning

confidence: 99%

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

Broeg

2003

Helgoland Marine Research

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The activity of acid phosphatase in liver macrophage aggregates (MA-AP) of different fish species was used as a marker for a pollution-induced modulation of the digestive capacity of phagocytes, since functions of the non-specific immune response play a central role in the maintenance of animals' health. Based upon the investigation of more than 900 individual flounders (Platichthys flesus) and mullets (Liza aurata), natural variations, gender-specific differences and pollution-induced alterations in AP activity are demonstrated in this study. MA-AP activity was dependent on temperature and season but, nevertheless, distinctions between differently polluted areas were visible in all sampling campaigns with lowest MA-AP activity in fish from the polluted areas of the German Bight and the Israeli coast of the Mediterranean Sea. For organochlorine contaminants, as well as for mercury and copper, a significant correlation could be observed between residue concentrations in fish tissues and MA-AP activity. In all cases, except mercury which showed a positive correlation, AP activity was suppressed in animals with a high contaminant burden. MA-AP activity turned out to give reliable and consistent results for a quantification of immunomodulation in both fish species.

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Acid phosphatases of Esox lucius: tissue distribution and partial characterization

Cited by 7 publications

References 20 publications

Novel findings in sea cucumber's digestive capacities: Enzymatic activities in the respiratory tree, implications for aquaculture

Novel findings in sea cucumber's digestive capacities: Enzymatic activities in the respiratory tree, implications for aquaculture

Characteristics of acid phosphatase from Russian sturgeon (Acipenser gueldenstaedtii) spermatozoa

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

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