Acid-shock of<i>Campylobacter jejuni</i>induces flagellar gene expression and host cell invasion

Le, My Thanh; Porcelli, Ida; Weight, C. M.; Gaskin, Duncan J. H.; Carding, Simon R.; Vliet, Arnoud H. M. van

doi:10.1556/eujmi.2.2012.1.3

Cited by 19 publications

(19 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the expression of the catalase katA gene was found to be responsive to acid stress by our microarray analysis (see Fig. S1 and Table S1 in the supplemental material), in agreement with the findings of previous studies, which revealed the upregulation of katA in response to acid exposure (20,25). The increased expression of katA might in turn enhance C. jejuni survival upon exposure to H 2 O 2 .…”

Section: Resultssupporting

confidence: 91%

“…The upregulation of KatA in acid-stressed C. jejuni bacteria suggests that acid exposure induces catalase expression, in agreement with the findings of previous studies (17,25). Moreover, the enhanced survival of the C. jejuni wild-type strain in the presence of H 2 O 2 following acid exposure, a finding that was not seen in either the ⌬fur or the ⌬katA mutant, suggests that both Fur and KatA are essential for the acidenhanced C. jejuni H 2 O 2 resistance.…”

Section: Discussionsupporting

confidence: 92%

“…These data indicate that Fur might play a key role in C. jejuni pathogenesis, especially upon exposure to acidic conditions, like those encountered in the host gastrointestinal tract. Indeed, deletion of the fur gene significantly reduced the capacity of C. jejuni to adhere to, invade, and survive intracellularly within HCT116 cells, which are important virulence traits of C. jejuni (25,53). Moreover, in wax worm larvae, the C. jejuni fur mutant had a significantly higher LD 50 than the wild-type strain.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress

et al. 2016

View full text Add to dashboard Cite

bCampylobacter jejuni is a prevalent cause of bacterial gastroenteritis in humans worldwide. The mechanisms by which C. jejuni survives stomach acidity remain undefined. In the present study, we demonstrated that the C. jejuni ferric uptake regulator (Fur) plays an important role in C. jejuni acid survival and acid-induced cross-protection against oxidative stress. A C. jejuni ⌬fur mutant was more sensitive to acid than the wild-type strain. Profiling of the acid stimulon of the C. jejuni ⌬fur mutant allowed us to uncover Fur-regulated genes under acidic conditions. In particular, Fur was found to upregulate genes involved in flagellar and cell envelope biogenesis upon acid stress, and mutants with deletions of these genes were found to be defective in surviving acid stress. Interestingly, prior acid exposure of C. jejuni cross-protected against oxidative stress in a catalase (KatA)-and Fur-dependent manner. Western blotting and reverse transcription-quantitative PCR revealed increased expression of KatA upon acid stress. Electrophoretic mobility shift assays (EMSAs) demonstrated that the binding affinity between Fur and the katA promoter is reduced in vitro under conditions of low pH, rationalizing the higher levels of expression of katA under acidic conditions. Strikingly, the ⌬fur mutant exhibited reduced virulence in both human epithelial cells and the Galleria mellonella infection model. Altogether, this is the first study showing that, in addition to its role in iron metabolism, Fur is an important regulator of C. jejuni acid responses and this function cross-protects against oxidative stress. Moreover, our results clearly demonstrate Fur's important role in C. jejuni pathogenesis.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress

et al. 2016

View full text Add to dashboard Cite

show abstract

“…2). Similar results were observed for other C. concisus strains (data not shown), as was for C. jejuni by Le et al (2012). We then examined the adherence to and invasion of gastric AGS cells by C. concisus strains.…”

Section: Resultssupporting

confidence: 84%

Campylobacter concisus utilizes blood but not short chain fatty acids despite showing associations with Firmicutes taxa

Kaakoush

Thomas²,

Ruzayqat

et al. 2016

Microbiology

View full text Add to dashboard Cite

“…In this study, the effect of acidic pH on the flgE gene expression in M. tianshanense was investigated. After a shift from pH 7 to pH 5, as described for Campylobacter jejuni [17], the flgE gene expression was measured in various growth phases, but no significant change in expression was observed (data not shown). This finding indicated that the picture of flgE gene expression in different strains, even closely related species, is ambiguous.…”

Section: Transcription Of the Flge Genementioning

confidence: 99%

Flagellar-Dependent Motility in Mesorhizobium tianshanense is Involved in the Early Stage of Plant Host Interaction: Study of an flgE Mutant

et al. 2014

View full text Add to dashboard Cite

Bacterial motility is most likely a critical factor for rhizobium to chemotactically colonize on the root surface prior to infecting leguminous plant hosts. Several studies of the rhizobium flagellar filament have been reported, but little is known about the rhizobium flagellum hook. To investigate the roles of the hook protein in flagellum synthesis in Mesorhizobium tianshanense, the hook protein-encoding gene flgE of M. tianshanense was amplified by PCR and sequenced. Comparison of the deduced amino acid sequences revealed pronounced similarities in Domain 1 and lower similarities in Domain 2, which are supposed to be related to hook structure assembly and antigenic diversity, respectively. The level of transcription of flgE increased along with the cell growth and reached its maximum at the middle log phase. Disruption of the flgE gene caused a flagellar-less phenotype, thereby causing complete loss of swimming ability, modified nutrient-related swarming ability and biofilm formation. Moreover, the absence of flagellar caused decreased bacterial attachment on the root hair, suggesting that flagellar is involved in the early stage of symbiosis process.

show abstract

Acid-shock ofCampylobacter jejuniinduces flagellar gene expression and host cell invasion

Cited by 19 publications

References 38 publications

The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress

The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress

Campylobacter concisus utilizes blood but not short chain fatty acids despite showing associations with Firmicutes taxa

Flagellar-Dependent Motility in Mesorhizobium tianshanense is Involved in the Early Stage of Plant Host Interaction: Study of an flgE Mutant

Contact Info

Product

Resources

About