Acid-soluble chromosomal proteins in maize root and callus cells and after rhizogenesis induction in callus tissues

Alterations in core histone variant ratios during maize root differentiation, callus formation and in response to plant hormone treatment

“…7), which was increased compared to meristematic zone and the increased ratio of uH2A in first callus culture derived from differentiation zone. These data concur with the fact that, despite morphological similarity observed in calli derived from divergent developmental stages, differences in biochemical properties continue to exist during plant cell dedifferentiation (Koleva et al 1982). The increased H3.2 ratio in the proliferating cells of callus cultures might be necessary in the proper function of cells due to its cell cycle-independent expression and ability to replace other histone H3 variants, a process that is possible to alter chromatin structures in specific regions.…”

“…Although histone variants and modifications have been identified and studied in plants (Waterborg, 1993, Jermanowski et al, 2000, Loild, 2004, little is known about their spatial distribution during plant cell differentiation and dedifferentiation, processes that involve several alterations in chromatin structure and function. However, alterations in chromatin composition between meristematic and differentiated plant cells and during callus formation have been observed in previous studies (Burkhanova et al, 1975, Koleva et al, 1982, Williams et al 2003.…”

“…The meristematic and elongation zones contain proliferating cells and primary root tissues respectively, whereas the differentiation zone consists mainly of parenchymatic, non-proliferating cells Hochholdinger et al, 2004). On the other hand, it is well established that during callus formation, differentiated plant cells are transformed into callus culture cells that regain the ability for rapid proliferation (Koleva et al, 1982, Williams et al, 2003. It is also known that plant hormones like auxin and gibberellin play significant roles in root growth by promoting or inhibiting root differentiation.…”

Μελέτη Του Προτύπου Κατανομής Των Ιστονών Κατά Την Διαφοροποίηση Φυτικών Κυττάρων

“…It is known that during callus formation, differentiated plant cells can be transformed into callus culture cells, which restore their meristematic properties, especially the ability for rapid proliferation (Koleva et al, 1982. According to the results presented in Figure 4B, the linker histone variants' ratios during plant cell dedifferentiation in callus culture were similar to meristematic zone, regardless of the developmental zone from which calli were derived.…”

“…Although histone variants and modifications have been identified and studied in plants , Jermanowski et al, 2000, Loild, 2004, little is known about their spatial distribution during plant cell differentiation and dedifferentiation, processes that involve several alterations in chromatin structure and function. However, alterations in chromatin composition between meristematic and differentiated plant cells and during callus formation have been observed in previous studies (Burkhanova et al, 1975, Koleva et al, 1982).…”