Acid treatment for RNA removal from yeast

A method for reduction of nucleic acid levels in preparations of the yeasts Saccharomyces cerevisiae and Kluyveromycesfragilis by means of alkaline treatment has been developed. Under similar conditions (4.5% NH4OH, 65 ° C, 30 min) a low nucleic acid content (less than 2%) was obtained for both strains. Higher losses of proteins and biomass were obtained with K. fragilis than with S. cerevisiae. 1977). The purpose of the present work was to obtain the reduction of nucleic acid levels in two yeast strains by a moderate treatment with NHaOH. Saccharomyces cerevisiae (baker's yeast), widely used in several foods, and Kluyveromyces fragilis, proposed as one of the more suitable yeasts for human consumption (Contreras and Vald6s 1984), were employed.

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Acid treatment for RNA removal from yeast

Cited by 6 publications

References 9 publications

Nucleic acid reduction in yeast

Nucleic acid reduction in yeast

Nucleic acid reduction in yeast

Nucleic acid reduction in yeast

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