Sludge production in wastewater treatment plants is increasing worldwide due to the increasing population. This work investigated the effects of ultrasonic (ULS), ultrasonic-ozone (ULS-Ozone) and ultrasonic + alkaline (ULS+ALK) post-treatments on the anaerobic digestion of sewage sludge in semi-continuous anaerobic reactors. Three conditions were tested with different hydraulic retention times (HRT, 10 or 20 days) and sludge recycle ratios (R = Q R /Q in (%): 50 or 100%). Biogas yield increased by 17.8% when ULS+ALK post-treatment was applied to the effluent of a reactor operating at 20 days HRT and at a 100% recycle ratio. Operation at 10 days HRT also improved the biogas yield (277 mL CH 4 /g VS added (VS: volatile solids) versus 249 mL CH 4 /g VS added in the control). The tested post-treatment methods showed 4-7% decrease in effluent VS. The post-treatment resulted in a decrease in the cellular ATP (Adenosine tri-phosphate) concentration indicating stress imposed on microorganisms in the reactor. Nevertheless, this did not prevent higher biogas production. Based on the results, the post-treatment of digested sludge or treating the sludge between two digesters is an interesting alternative to pre-treatments.