Acidification by gluconic acid of mango fruit tissue during colonization via stem end infection by Phomopsis mangiferae

Davidzon, Maayan; Alkan, Noam; Kobiler, Ilana; Prusky, D.

doi:10.1016/j.postharvbio.2009.08.009

Cited by 36 publications

(22 citation statements)

References 29 publications

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“…Infection of microscopic fungus manifested itself as typical spores, whose development occur on the surface and can be isolated from pure culture. This microscopic fungus is a typical apple causal agent (Davidzon et al, 2010). The initial systematic infection usually occurs during apple flowering due to the fact that Phomopsis spp., advantageously colonise dead limbs and trunks.…”

Section: Resultsmentioning

confidence: 99%

Microbiological changes and severity of decay in apples stored for a long-term under different storage conditions

Juhnevica-Radenkova

Radenkovs

Segliņa

2016

Zemdirbyste-Agriculture

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Apples occupy most of the Latvian vegetable market, nevertheless there is a lack of good quality locally grown apples. Using advanced storage technologies, such as storage in controlled atmosphere in ultra low oxygen (O 2 ) (ULO) and elevated carbon dioxide (CO 2 ) conditions, as well as pre-treatment of fruits with 1-Methylcyclopropene (1-MCP) will substantially improve the quality of fruits and globally assist apple growers to distribute more qualitative and safe fruits on the local market. The aim of the study was to ascertain the causes of apple decay that occurred during storage under different conditions. Two apple storage technologies were tested in this study: cold storage under conventional conditions + 1-Methylcyclopropene treatment and controlled atmosphere -2.0% CO 2 , 1.0% O 2 (ULO1) and 2.5% CO 2 , 1.5% O 2 (ULO2) conditions. After apple storage for six months the following microscopic fungi were isolated: Penicillium expansum (30.44%), Monilinia fructigena (26.08%), Neofabraea alba (21.73%), Colletotrichum acutatum (13.04%), Botrytis cinerea (8.69%) in cold storage, while P. expansum (35.26%), Colletotrichum acutatum (23.57%), Neofabraea alba (11.76%), B. cinerea (11.76%), M. fructigena (11.76%) and Fusarium avenaceum (5.89%) were isolated from fruits that prior to storage were pre-treated with 1-MCP. Apples that had been stored under ULO1 conditions predominantly were contaminated with: N. alba (33.34%), M. fructigena (26.69%), B. cinerea (13.33%), P. expansum (6.66%), F. avenaceum (6.66%), Phomopsis/ Diaporthe eres (6.66%) and Mucor circinelloides (6.66%), while under ULO2: N. alba (33.33%), M. fructigena (33.33%), F. avenaceum (16.67%) and P./D. eres (16.67%). Diversity of microscopic fungi, which were isolated from differently stored and decayed apple samples, was quite similar, though with insignificant difference. For instance, microscopic fungus F. avenaceum was not found only in cold storage kept fruits, while microscopic fungus of the genus P./D. eres was identified when apples were stored under ultra low oxygen conditions.

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Section: Resultsmentioning

confidence: 99%

Microbiological changes and severity of decay in apples stored for a long-term under different storage conditions

Juhnevica-Radenkova

Radenkovs

Segliņa

2016

Zemdirbyste-Agriculture

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“…In addition, gluconic acid intensity increase might also cause by the effect of cell wall degradation, change in cuticle composition and pH of host cells that allow the transition of fungi into their aggressive colonization [44]. The presence of gluconic acid might indicate an infection that could secrets gluconic acid and acidify the pH in fruit such as in apple and mango [45,46].…”

Section: Discussionmentioning

confidence: 99%

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

et al. 2020

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Pineapple is one of the most cultivated tropical, non-climacteric fruits in the world due to its high market value and production volume. Since non-climacteric fruits do not ripen after harvest, the ripening stage at the time of harvest is an important factor that determines sensory quality and shelf life. The objective of this research was to investigate metabolite changes in the pineapple ripening process by metabolite profiling approach. Pineapple (Queen variety) samples from Indonesia were subjected to GC-MS analysis. A total of 56, 47, and 54 metabolites were annotated from the crown, flesh, and peel parts, respectively. From the principal component analysis (PCA) plot, separation of samples based on ripening stages from C0–C2 (early ripening stages) and C3–C4 (late ripening stages) was observed for flesh and peel parts, whereas no clear separation was seen for the crown part. Furthermore, orthogonal projection to latent structures (OPLS) analysis suggested metabolites that were associated with the ripening stages in flesh and peel parts of pineapple. This study indicated potentially important metabolites that are correlated to the ripening of pineapple that would provide a basis for further study on pineapple ripening process.

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“…The ability of postharvest pathogens to alter pH locally was initially described for Colletotrichum gloeosporioides , and then extended to some other pathogens, such as Alternaria alternata , Botrytis cinerea , Penicillium expansum , Penicillium digitatum , Penicillium italicum, Phomopsis mangiferae, Monilinia fructicola , and Fusarium oxysporum [ 2 – 10 ]. Attacking pathogenic fungi such as P. expansum , P. digitatum , P. italicum [ 7 ], Phomopsis mangiferae [ 2 ], B. cinerea [ 5 ], and Sclerotinia sclerotiorum [ 11 ] acidify tissue with organic acids. Fungi can also achieve ambient alkalization by actively secreting ammonia, which results from protease activation followed by amino acid deamination [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Differential gene expression in tomato fruit and Colletotrichum gloeosporioides during colonization of the RNAi–SlPH tomato line with reduced fruit acidity and higher pH

et al. 2017

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BackgroundThe destructive phytopathogen Colletotrichum gloeosporioides causes anthracnose disease in fruit. During host colonization, it secretes ammonia, which modulates environmental pH and regulates gene expression, contributing to pathogenicity. However, the effect of host pH environment on pathogen colonization has never been evaluated. Development of an isogenic tomato line with reduced expression of the gene for acidity, SlPH (Solyc10g074790.1.1), enabled this analysis. Total RNA from C. gloeosporioides colonizing wild-type (WT) and RNAi–SlPH tomato lines was sequenced and gene-expression patterns were compared.Results C. gloeosporioides inoculation of the RNAi–SlPH line with pH 5.96 compared to the WT line with pH 4.2 showed 30% higher colonization and reduced ammonia accumulation. Large-scale comparative transcriptome analysis of the colonized RNAi–SlPH and WT lines revealed their different mechanisms of colonization-pattern activation: whereas the WT tomato upregulated 13-LOX (lipoxygenase), jasmonic acid and glutamate biosynthesis pathways, it downregulated processes related to chlorogenic acid biosynthesis II, phenylpropanoid biosynthesis and hydroxycinnamic acid tyramine amide biosynthesis; the RNAi–SlPH line upregulated UDP-D-galacturonate biosynthesis I and free phenylpropanoid acid biosynthesis, but mainly downregulated pathways related to sugar metabolism, such as the glyoxylate cycle and L-arabinose degradation II. Comparison of C. gloeosporioides gene expression during colonization of the WT and RNAi–SlPH lines showed that the fungus upregulates ammonia and nitrogen transport and the gamma-aminobutyric acid metabolic process during colonization of the WT, while on the RNAi–SlPH tomato, it mainly upregulates the nitrate metabolic process.ConclusionsModulation of tomato acidity and pH had significant phenotypic effects on C. gloeosporioides development. The fungus showed increased colonization on the neutral RNAi–SlPH fruit, and limited colonization on the WT acidic fruit. The change in environmental pH resulted in different defense responses for the two tomato lines. Interestingly, the WT line showed upregulation of jasmonate pathways and glutamate accumulation, supporting the reduced symptom development and increased ammonia accumulation, as the fungus might utilize glutamate to accumulate ammonia and increase environmental pH for better expression of pathogenicity factors. This was not found in the RNAi–SlPH line which downregulated sugar metabolism and upregulated the phenylpropanoid pathway, leading to host susceptibility.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3961-6) contains supplementary material, which is available to authorized users.

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Acidification by gluconic acid of mango fruit tissue during colonization via stem end infection by Phomopsis mangiferae

Cited by 36 publications

References 29 publications

Microbiological changes and severity of decay in apples stored for a long-term under different storage conditions

Microbiological changes and severity of decay in apples stored for a long-term under different storage conditions

GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Ananas comosus)

Differential gene expression in tomato fruit and Colletotrichum gloeosporioides during colonization of the RNAi–SlPH tomato line with reduced fruit acidity and higher pH

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