Acidithiobacillus ferrooxidans oxidizes ferrous iron before sulfur likely through transcriptional regulation by the global redox responding RegBA signal transducing system

Ponce, Jose; Moinier, Danielle; Byrne, Deborah; Amouric, Agnès; Bonnefoy, Violaine

doi:10.1016/j.hydromet.2012.07.016

Cited by 39 publications

(46 citation statements)

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“…Expression of petA1B1 during cultivation of A. ferrivorans SS3 indicated that they were induced by the presence of Fe 2ϩ . This is consistent with previous studies showing petA1B1 upregulation on Fe 2ϩ as a sole energy source (34,35 (13), and they support the view that Fe 2ϩ is the preferred substrate among Fe 2ϩ -oxidizing acidithiobacilli (15). However, reports of the preferred substrate for A. ferrooxidans are conflicting, since S 0 was oxidized by A. ferrooxidans FC1 on the surface of zinc sulfide in the presence of Fe 2ϩ concentrations ranging from 1 to 9.5 g/liter (36).…”

Section: Discussionsupporting

confidence: 91%

“…In contrast to sulfur oxidation by A. ferrooxidans, Acidithiobacillus caldus oxidation of S 0 is suggested to be mediated by sulfur oxygenase reductase (Sor) and thiosulfate oxidation by the periplasmic thiosulfate-oxidizing multienzyme system (core TOMES) (14). A. ferrooxidans T cultured in a mixed Fe 2ϩ -S 0 medium immediately transcribed Fe 2ϩ -associated genes, whereas S 0 -related genes were first transcribed when all the Fe 2ϩ was oxidized (15). RegAB, a redox-responsive two-component system of A. ferrooxidans T , downregulates genes encoding S 0 oxidation proteins in the presence of Fe 2ϩ (15).…”

mentioning

confidence: 99%

“…A. ferrooxidans T cultured in a mixed Fe 2ϩ -S 0 medium immediately transcribed Fe 2ϩ -associated genes, whereas S 0 -related genes were first transcribed when all the Fe 2ϩ was oxidized (15). RegAB, a redox-responsive two-component system of A. ferrooxidans T , downregulates genes encoding S 0 oxidation proteins in the presence of Fe 2ϩ (15). The A. ferrivorans SS3 genome sequence contains the rus and petI operons, associated with Fe 2ϩ oxidation, and genes for the ISC-metabolizing enzymes tetrathionate hydrolase (tetH), sulfide quinone reductase (sqr), thiosulfate-quinone oxidoreductase (doxDA), sulfur oxidation complex SOX (soxYZ-hypB), and Sor (16).…”

mentioning

confidence: 99%

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Gene Identification and Substrate Regulation Provide Insights into Sulfur Accumulation during Bioleaching with the Psychrotolerant Acidophile Acidithiobacillus ferrivorans

Liljeqvist

Rzhepishevska

Dopson

2013

Appl Environ Microbiol

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The psychrotolerant acidophile Acidithiobacillus ferrivorans has been identified from cold environments and has been shown to use ferrous iron and inorganic sulfur compounds as its energy sources. A bioinformatic evaluation presented in this study suggested that Acidithiobacillus ferrivorans utilized a ferrous iron oxidation pathway similar to that of the related species Acidithiobacillus ferrooxidans. However, the inorganic sulfur oxidation pathway was less clear, since the Acidithiobacillus ferrivorans genome contained genes from both Acidithiobacillus ferrooxidans and Acidithiobacillus caldus encoding enzymes whose assigned functions are redundant. Transcriptional analysis revealed that the petA1 and petB1 genes (implicated in ferrous iron oxidation) were downregulated upon growth on the inorganic sulfur compound tetrathionate but were on average 10.5-fold upregulated in the presence of ferrous iron. In contrast, expression of cyoB1 (involved in inorganic sulfur compound oxidation) was decreased 6.6-fold upon growth on ferrous iron alone. Competition assays between ferrous iron and tetrathionate with Acidithiobacillus ferrivorans SS3 precultured on chalcopyrite mineral showed a preference for ferrous iron oxidation over tetrathionate oxidation. Also, pure and mixed cultures of psychrotolerant acidophiles were utilized for the bioleaching of metal sulfide minerals in stirred tank reactors at 5 and 25°C in order to investigate the fate of ferrous iron and inorganic sulfur compounds. Solid sulfur accumulated in bioleaching cultures growing on a chalcopyrite concentrate. Sulfur accumulation halted mineral solubilization, but sulfur was oxidized after metal release had ceased. The data indicated that ferrous iron was preferentially oxidized during growth on chalcopyrite, a finding with important implications for biomining in cold environments.

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Section: Discussionsupporting

confidence: 91%

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confidence: 99%

mentioning

confidence: 99%

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Gene Identification and Substrate Regulation Provide Insights into Sulfur Accumulation during Bioleaching with the Psychrotolerant Acidophile Acidithiobacillus ferrivorans

Liljeqvist

Rzhepishevska

Dopson

2013

Appl Environ Microbiol

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“…The pH level influences the ratio by changing the degree of ferric hydrolysis (Daoud and Karamanev, 2006), while chelators affect the ratio by changing the effective Fe 3þ concentrations via formation of ferric complexes. In previous research, A. ferrooxidans has been found to have a redox sensing system RegB-RegA that can sense redox states (ORP) and regulate gene expression to transition between iron and sulfur oxidation (Ponce et al, 2012;Quatrini et al, 2009). Here, it is very like that cells sense the change in ORP to regulate other metabolic activities that impact IBA production.…”

Section: Discussionmentioning

confidence: 99%

Enhancing isobutyric acid production from engineered Acidithiobacillus ferrooxidans cells via media optimization

West²,

Banta³

2015

Biotech & Bioengineering

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The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans has previously been genetically modified to produce isobutyric acid (IBA) from carbon dioxide while obtaining energy from the oxidation of ferrous iron. Here, a combinatorial approach was used to explore the influence of medium composition in both batch and chemostat cultures in order to improve IBA yields (g IBA/mol Fe(2+)) and productivities (g IBA/L/d). Medium pH, ferrous concentration (Fe(2+)), and inclusion of iron chelators all had positive impact on the IBA yield. In batch experiments, gluconate was found to be a superior iron chelator because its use resulted in smaller excursions in pH. In batch cultures, IBA yields decreased linearly with increases in the final effective Fe(3+) concentrations. Chemostat cultures followed similar trends as observed in batch cultures. Specific cellular productivities were found to be a function of the steady state ORP (Oxidation-reduction potential) of the growth medium, which is primarily determined by the Fe(3+) to Fe(2+) ratio. By operating at low ORP, chemostat cultures were able to achieve volumetric productivities as high as 3.8 ± 0.2 mg IBA/L/d which is a 14-fold increase over the previously reported value.

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“…Some A.ferrooxidans strains have been reported to prefer to oxidize ferrous iron prior to sulfur when both substrates are available, while others oxidize the two substrates simultaneously (Ponce et al, 2012;Zhang et al, 2013). In order to investigate the effect of rock phosphates added in different leaching systems on bacterial growth, the change of cell concentration over time with rock phosphates added was studied.…”

Section: Effect Of Rock Phosphates On Bacterial Growth Under Differenmentioning

confidence: 99%

Effect of energy source and leaching method on bio-leaching of rock phosphates by Acidithiobacillus ferrooxidans

Zuo

et al. 2016

Hydrometallurgy

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Acidithiobacillus ferrooxidans oxidizes ferrous iron before sulfur likely through transcriptional regulation by the global redox responding RegBA signal transducing system

Cited by 39 publications

References 18 publications

Gene Identification and Substrate Regulation Provide Insights into Sulfur Accumulation during Bioleaching with the Psychrotolerant Acidophile Acidithiobacillus ferrivorans

Gene Identification and Substrate Regulation Provide Insights into Sulfur Accumulation during Bioleaching with the Psychrotolerant Acidophile Acidithiobacillus ferrivorans

Enhancing isobutyric acid production from engineered Acidithiobacillus ferrooxidans cells via media optimization

Effect of energy source and leaching method on bio-leaching of rock phosphates by Acidithiobacillus ferrooxidans

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