ACIDITY MEASUREMENTS WITH THE GLASS ELECTRODE IN H<sub>2</sub>O-D<sub>2</sub>O MIXTURES

Mikkelsen, Kirsten; Nielsen, Sigurd O.

doi:10.1021/j100834a026

Cited by 244 publications

(160 citation statements)

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“…The correction pD = pH measured + 0.4 was applied to the pH meter readings [15,16]. Kinetic runs were run in duplicate to give a reproducibility of ±15%.…”

Section: Uv Kinetic Measurementsmentioning

confidence: 99%

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

Montagner

Gandin

Marzano

et al. 2015

Journal of Inorganic Biochemistry

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The dinuclear copper(II) complex [Cu 2 {bcmp(-H)}(μ-OH)](NO 3 ) 2 ·H 2 O (1, bcmp = 2,6-bis(1,4,7-triazacyclonon-1-ylmethyl)-4-methylphenol) has been synthesized and characterized by electrospray ionization mass spectrometry, potentiometric titration and cyclovoltammetry. The X-ray structure of the analogous perchlorate salt [Cu 2 {bcmp(-H)}(μ-OH)](ClO 4 ) 2 ·2.5H 2 O (2) was determined. Cytotoxicity studies showed very promising activity of 1 against various pancreatic tumor cell lines with IC 50 values comparable or even lower than those of cisplatin. The Cu complex displayed low toxicity against a human non-tumor cell line (HEK 293) demonstrating selectivity for cancer cells. 1 converts supercoiled pUC19 plasmid DNA into the nicked form at micromolar concentrations in the absence of added reductants. A detailed kinetic study on the hydrolysis of the DNA model bis(2,4-dinitrophenyl) phosphate (BDNPP) has been performed. 1 hydrolyses BDNPP with a second order rate constant of 0.047 M s −1 at pH 8 and 40°C. Finally, single cell electrophoresis (comet assay) and fluorescence microscopy analysis showed that 1 interacts with cellular DNA and induces apoptotic cell death of Capan-1 pancreatic cancer cells. Western blotting analysis indicated that the Cu complex activates the p53 dependent pathway of apoptosis.

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“…The correction pD = pH measured + 0.4 was applied to the pH meter readings [15,16]. Kinetic runs were run in duplicate to give a reproducibility of ±15%.…”

Section: Uv Kinetic Measurementsmentioning

confidence: 99%

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

Montagner

Gandin

Marzano

et al. 2015

Journal of Inorganic Biochemistry

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“…1,4,7-Triazacyclononane (tacn) was synthesized according to a modified literature procedure (Supporting Information). [56] 1 H and 31 P NMR spectra were recorded on a Jeol EXC-400 spectrometer and referenced to TMS or the solvent residual peak as [57,58] The pD values were adjusted using concentrated DNO 3 and NaOD solutions. UV/Vis measurements were carried out on a Varian Cary 50 scan spectrophotometer coupled to a Grant thermostatted water circulation bath.…”

Section: Nnјnјј-tris(p-tolylsulfonyl)-147-triazacyclononanementioning

confidence: 99%

“…The correction pD = pH measured + 0.4 was applied to the pH meter readings. [57,58] Kinetic runs were run in duplicate to give a reproducibility of Ϯ15 %.…”

Section: 6-mentioning

confidence: 99%

Phosphate Diester Cleavage, DNA Interaction and Cytotoxic Activity of a Bimetallic Bis(1,4,7‐triazacyclononane) Zinc Complex

Montagner¹,

Gandin²,

Marzano³

et al. 2014

Eur J Inorg Chem

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Keywords: Drug design / Antitumor agents / Cytotoxicity / Zinc / DNA / DNA cleavageThe dinuclear zinc complex [Zn 2 {bcmp(-H)}(μ-Cl)](ClO 4 ) 2 · H 2 O {bcmp = 2,6-bis(1,4,7-triazacyclonon-1-ylmethyl)-4-methylphenol} has been synthesized and structurally characterized. The DNA binding affinity was assessed by ethidium bromide fluorescence quenching experiments. The complex relaxes supercoiled pUC19 DNA into the nicked form at low micromolar concentration. Mechanistic studies were carried out using the DNA and RNA models bis(2,4-dinitrophenyl) phosphate (BDNPP) and 2-hydroxypropyl p-nitrophenyl

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“…Instead of comparative measurements on two separately prepared samples, the RCs from a highly concentrated stock (N 300 μM) in H 2 O (or D 2 O) were diluted into D 2 O (or H 2 O) gradually while the salt and detergent concentrations were held constant. The observed rates were plotted as a function of dilution and a linear fit to the measured rates offered k -ion concentration for the glass electrode solvent isotope artifact [32,33]. The "apparent pH" means the actual pH meter reading.…”

Section: Hydrogen Isotope Measurementsmentioning

confidence: 99%

“…sphaeroides, 183 the cultivation, and the preparation of RC protein have been 184 described previously. 19 The UQ at the Q B site was removed as 185 described previously 32 and reconstituted by addition of RQ in 186 large (>10) excess over RC. The occupancy of the Q B site was 187 >70% as determined from the ratio of the amplitudes of the 188 slow and fast phases of the charge recombination measured at 189 865 nm.…”

Section: áGnesmentioning

confidence: 99%

Proton transfer in bioenergetic proteins

Maróti¹

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ACIDITY MEASUREMENTS WITH THE GLASS ELECTRODE IN H₂O-D₂O MIXTURES

Cited by 244 publications

References 0 publications

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

Phosphate Diester Cleavage, DNA Interaction and Cytotoxic Activity of a Bimetallic Bis(1,4,7‐triazacyclononane) Zinc Complex

Proton transfer in bioenergetic proteins

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ACIDITY MEASUREMENTS WITH THE GLASS ELECTRODE IN H2O-D2O MIXTURES

Cited by 244 publications

References 0 publications

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex

Phosphate Diester Cleavage, DNA Interaction and Cytotoxic Activity of a Bimetallic Bis(1,4,7‐triazacyclononane) Zinc Complex

Proton transfer in bioenergetic proteins

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Product

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ACIDITY MEASUREMENTS WITH THE GLASS ELECTRODE IN H₂O-D₂O MIXTURES