Acidity reduction of enzymatic biodiesel using alkaline washing

Uliana, Nilva Regina; Polloni, André E.; Paliga, Marshall; Veneral, Josamaique G.; Quadri, Marintho Bastos; Oliveira, J. Vladimir

doi:10.1016/j.renene.2017.06.001

Cited by 11 publications

(5 citation statements)

References 19 publications

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Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

“…Recently, papers have been focused on the reduction of the biodiesel acidity produced by liquid lipase-mediated hydroesterification [144][145][146][147]. In one of these researches, Uliana et al [144] proposed an alkaline wet washing using aqueous solution of sodium hydroxide/methanol as a technique for reducing the biodiesel acidity produced by enzymatic hydroesterification: under 35 • C, 1 wt% of methanol, molar ratio NaOH:FFA of 1:1 and 3 wt% of alkaline solution in relation to crude biodiesel, the authors reduced the FAME acidity from 3.94 to 0.19 wt% for a final product yield of 96%. In a similar approach, Firdaus et al [31] utilized a caustic washing to bring the biodiesel produced by soluble lipase-mediated hydroesterification to on-spec parameters: after the alkaline washing, the FFA content decreased from 2.0 to 0.04 wt%, with an increase in the biodiesel yield from 96.7% to 99.4%, where the residual MAG contained in the FAME sample could be transesterified, rising the process yield.…”

Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

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Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.

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Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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“…Considering that this enzyme has been designed to produce biodiesel [31][32][33][34][35][36][37][38][39], it is surprising that methanol, a substrate in this reaction, is the one with a higher negative effect on enzyme stability when compared to CALB. However, it may be expected that in anhydrous media the effect of methanol may be different, but this relatively poor stability of the Eversa enzyme in methanol should be considered in the biodiesel reaction design.…”

Section: Inactivation Of the Enzyme Preparations In Organic Solventsmentioning

confidence: 99%

“…Since then, the enzyme has been mainly used in biodiesel production [31][32][33][34][35][36][37][38]. In all these papers, the enzyme has been used in free form as recommended by the supplier.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the main objective of the current paper is to show a first evaluation of the properties of Eversa, immobilized on octyl agarose beads and treated with PEI, under different conditions, which may be expected that will have a likely impressive impact in the next future due to some of their properties [31][32][33][34][35][36][37][38][39]. Eversa has been also ionically exchanged in aminated agarose for comparison with octyl agarose.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features

2018

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Eversa is an enzyme recently launched by Novozymes to be used in a free form as biocatalyst in biodiesel production. This paper shows for first time the immobilization of Eversa (a commercial lipase) on octyl and aminated agarose beads and the comparison of the enzyme properties to those of the most used lipase, the isoform B from Candida antarctica (CALB) immobilized on octyl agarose beads. Immobilization on octyl and aminated supports of Eversa has not had a significant effect on enzyme activity versus p-nitrophenyl butyrate (pNPB) under standard conditions (pH 7), but immobilization on octyl agarose beads greatly enhanced the stability of the enzyme under all studied conditions, much more than immobilization on aminated support. Octyl-Eversa was much more stable than octyl-CALB at pH 9, but it was less stable at pH 5. In the presence of 90% acetonitrile or dioxane, octyl-Eversa maintained the activity (even increased the activity) after 45 days of incubation in a similar way to octyl-CALB, but in 90% of methanol, results are much worse, and octyl-CALB became much more stable than Eversa. Coating with PEI has not a clear effect on octyl-Eversa stability, although it affected enzyme specificity and activity response to the changes in the pH. Eversa immobilized octyl supports was more active than CALB versus triacetin or pNPB, but much less active versus methyl mandelate esters. On the other hand, Eversa specificity and response to changes in the medium were greatly modulated by the immobilization protocol or by the coating of the immobilized enzyme with PEI. Thus, Eversa may be a promising biocatalyst for many processes different to the biodiesel production and its properties may be greatly improved following a suitable immobilization protocol, and in some cases is more stable and active than CALB.

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Nonconventional Lipid Sources for Biodiesel Production

Bueno-Borges¹,

Sangaletti‐Gerhard²,

Camargo³

et al. 2020

Bailey's Industrial Oil and Fat Products

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In the beginning, the diesel engine ran on vegetable oil, and it was peanut oil, an edible oil, that was first used for this purpose. As fossil fuels may become scarce and prices could escalate, soybean, canola/rapeseed, and other known oils are being used. As the discussion on food security, environmental protection, and energy generation continues, alternative sources of oils or fats for biodiesel synthesis are being studied. Waste frying oil and animal fats have also been used as feedstock for biodiesel production at the same time as nonedible oils ( Jatropha , castor), oil industrial plants coproduct (palm fatty acid distillate), and unconventional lipid sources (microbial oils and sewage sludge) have now been brought into light. When it comes to long‐term implementation of adoption in a commercial scale one of these so‐called nonconventional lipid sources, what has been observed is that continuous production of regular quality feedstock, adequate infrastructure for collecting or harvesting, storing, processing, and transporting (logistics) prevails; only cost‐effective choices have proven successful.

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Acidity reduction of enzymatic biodiesel using alkaline washing

Cited by 11 publications

References 19 publications

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipases in liquid formulation for biodiesel production: Current status and challenges

Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features

Nonconventional Lipid Sources for Biodiesel Production

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