Acidosis in muscle tissue distal to vascular contusion despite unchanged global blood flow in rats: An uncoupling of microvascular blood flow and metabolism?

Karlander, Lars-Erik; Henriksson, Jan; Sjöberg, Folke

doi:10.1016/j.mvr.2005.06.003

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…These effects were particularly evident after blunt trauma to the neurovascular bundle. 25 Similarly, in our model, the vascular bundle is skeletonized and the accompanying nerve transected during flap preparation. These findings together suggest an involvement of neurovascular mechanisms in the paradoxical increase in tissue oxygenation in the flap during venous occlusion.…”

Section: Discussionmentioning

confidence: 99%

Vascular Occlusion in a Porcine Flap Model: Effects on Blood Cell Concentration and Oxygenation

Bergkvist

Zötterman

Henricson

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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Background:Venous congestion in skin flaps is difficult to detect. This study evaluated the ability of tissue viability imaging (TiVi) to measure changes in the concentration of red blood cells (CRBC), oxygenation, and heterogeneity during vascular provocations in a porcine fasciocutaneous flap model.Methods:In 5 pigs, cranial gluteal artery perforator flaps were raised (8 flaps in 5 pigs). The arterial and venous blood flow was monitored with ultrasonic flow probes. CRBC, tissue oxygenation, and heterogeneity in the skin were monitored with TiVi during baseline, 50% and 100% venous occlusion, recovery, 100% arterial occlusion and final recovery, thereby simulating venous and arterial occlusion of a free fasciocutaneous flap. A laser Doppler probe was used as a reference for microvascular perfusion in the flap.Results:During partial and complete venous occlusion, increases in CRBC were seen in different regions of the flap. They were more pronounced in the distal part. During complete arterial occlusion, CRBC decreased in all but the most distal parts of the flap. There were also increases in tissue oxygenation and heterogeneity during venous occlusion.Conclusions:TiVi measures regional changes in CRBC in the skin of the flap during arterial and venous occlusion, as well as an increase in oxygenated hemoglobin during venous occlusion that may be the result of reduced metabolism and impaired delivery of oxygen to the tissue. TiVi may provide a promising method for measuring flap viability because it is hand-held, easy to-use, and provides spatial information on venous congestion.

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Section: Discussionmentioning

confidence: 99%

Vascular Occlusion in a Porcine Flap Model: Effects on Blood Cell Concentration and Oxygenation

Bergkvist

Zötterman

Henricson

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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“…Lactate increase over the duration of the anaesthetic procedure (Cornish & Moon 1985) was affected in part by tissue damage and local hypoxic conditions (Soivio et al. 1981; Karlander et al. 2005) as a result of repeated phlebotomies and application of the forceps rather than changes related to water oxygenation or respiration.…”

Section: Discussionmentioning

confidence: 99%

“…Lactate concentration had a significant association with all time-dependent variables including anaesthetic duration, number of phlebotomies, and clamping events performed on each fish. Lactate increase over the duration of the anaesthetic procedure (Cornish & Moon 1985) was affected in part by tissue damage and local hypoxic conditions (Soivio et al 1981;Karlander et al 2005) as a result of repeated phlebotomies and application of the forceps rather than changes related to water MS-222 in Koi (Cyprinus carpio) J Stockman et al…”

Section: Ms-222 Inmentioning

confidence: 99%

Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)

Stockman

Weber

Kass

et al. 2013

Veterinary Anaesthesia and Analgesia

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“…Ethanol is well known and is commonly used by other authors. 17,18 Our experience with gentamycine as a flow marker was published in a previous publication. 4 Regarding the changes in dialysate concentrations of ethanol as the flow-marker and changes related to individual baselines, we have found no significant difference in microvascular tissue blood flow during surgery in either of the groups.…”

Section: Discussionmentioning

confidence: 99%

Impact of cardiopulmonary bypass on peripheral tissue metabolism and microvascular blood flow

et al. 2008

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The aim of this study was to monitor and compare the changes in metabolism and blood flow in the skeletal muscles during cardiac operations performed with cardiopulmonary bypass (CPB) and operations without CPB (off-pump) by means of interstitial microdialysis (Figure 1). Surgical revascularization, coronary artery bypass grafting (CABG), was performed in 40 patients randomized to two groups. Twenty patients (On-Pump Group) were operated on using CPB, 20 patients (Off-Pump Group) were operated on without CPB. Interstitial microdialysis was performed by 2 probes of a CMA 60 (CMA Microdialysis AB, Solna, Sweden) inserted into the patient's deltoid muscle. Microdialysis measurements were performed at 30-minute intervals. Glucose, lactate, pyruvate and glycerol as markers of basic metabolism and tissue perfusion were measured in samples from the first probe, using a CMA 600 Analyzer (CMA Microdialysis AB). Blood flow through the interstitium was monitored by means of dynamic microdialysis of ethanol as a flow-marker in the dialysates taken from the second probe (ethanol dilution technique). Results in both the groups were statistically processed and compared. Both the groups were similar in respect of preoperative characteristics. Dynamic changes of interstitial concentrations of the measured analytes were found in both the patient groups (on-pump vs. off-pump) during the operation. There was no significant difference in dialysate concentrations of glucose and lactate between the groups. Significant differences were detected in pyruvate and glycerol interstitial concentrations, lactate/pyruvate ratio and lactate/glucose ratio between the on-pump vs. off-pump patients. In the Off-Pump Group, pyruvate concentrations were higher and the values of concentrations of glycerol lower. The lactate/pyruvate ratio and the lactate/glucose ratio, indicating the aerobic and anaerobic tissue metabolism status, were lower in the Off-Pump Group. There was no significant difference in dialysate concentrations of ethanol as a flow-marker during the surgery in either of the groups. There was no statistically significant difference between the groups (On-Pump Group vs. Off-Pump Group) comparing the postoperative clinical outcome (ICU stay, ventilation duration, length of hospital stay). The dynamic changes in the interstitial concentrations of the glucose, glycerol, pyruvate and lactate were found in both the groups of patients (On-Pump Group and Off-Pump Group), but there was no difference in local blood flow when the ethanol dilution technique was used. These results showed significantly higher aerobic metabolic activity of the peripheral tissue of patients in the Off-Pump Group vs. the On-Pump Group during the course of cardiac revascularization surgery. Results suggest that extracorporeal circulation, cardiopulmonary bypass, compromises peripheral tissue (skeletal muscles) energy metabolism. These changes have no impact on the postoperative clinical outcome; no significant difference between the groups was found.

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Acidosis in muscle tissue distal to vascular contusion despite unchanged global blood flow in rats: An uncoupling of microvascular blood flow and metabolism?

Cited by 6 publications

References 19 publications

Vascular Occlusion in a Porcine Flap Model: Effects on Blood Cell Concentration and Oxygenation

Vascular Occlusion in a Porcine Flap Model: Effects on Blood Cell Concentration and Oxygenation

Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)

Impact of cardiopulmonary bypass on peripheral tissue metabolism and microvascular blood flow

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