Acidovorax anthurii sp. nov., a new phytopathogenic bacterium which causes bacterial leaf-spot of anthurium.

Abstract

The bacterial leaf-spot of anthurium emerged during the 1980s, in the French West Indies and Trinidad. This new bacterial disease is presently wide spread and constitutes a serious limiting factor for commercial anthurium production. Twenty-nine strains isolated from leaf-spots of naturally infected anthurium were characterized and compared with reference strains belonging to the Comamonadaceae family, the genera Ralstonia and Burkholderia, and representative fluorescent pseudomonads.

Verminephrobacter eiseniae gen. nov., sp. nov., a nephridial symbiont of the earthworm Eisenia foetida (Savigny)

“…DNA samples from A. temperans and A. delafieldii were used as controls. The DNA G+C content (determined for isolates EF01-1, EF01-2 T , EF02-2 and EF05-1) was 67.0±0.2 mol%, well within the 60-72 mol% values reported for other species of the genus Acidovorax (Schulze et al, 1999;Gardan et al, 2000Gardan et al, , 2003, and in close agreement with the 65.2 mol% observed for the genome sequence of isolate EF01-2 (unpublished observations).…”

“…Acidovorax-like 16S rRNA gene sequences are frequently recovered from wastewater treatment plants and other aquatic environments (Schweitzer et al, 2001;Hoshino et al, 2005;Manefield et al, 2005). The genus Acidovorax includes species isolated from soil and sludge (Willems et al, 1990;Schulze et al, 1999;Heylen et al, 2008), in addition to a group of plant pathogens with a varied host range (Willems et al, 1992;Gardan et al, 2000Gardan et al, , 2003. The present study reports the isolation and characterization of a relative of the genus Acidovorax from the nephridia of E. foetida.…”

Reclassification of subspecies of Acidovorax avenae as A. Avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov.

“…A. anthurii and A. valerianellae were easily separated from A. avenae. Unlike A. avenae, A. anthurii did not utilize L-tryptophan [11] and A. valerianellae failed to utilize D-sorbitol, adipate, ethanolamine, reduce nitrate, liquefy gelatin, or produce lipase [this study , 12]. Neither A. anthurii nor A. valerianellae utilized D-xylose, or produced alkaline from litmus milk, whereas A. avenae did (Table S-3).…”

“…konjaci [15] from konjac were classified as A. konjaci [55]. Recently two new phytopathogenic species of Acidovorax, A. anthurii on anthurium (Anthurium palmatum (L.) G. Don) [11] and A. valerianellae on lambs' lettuce (Valerianella locusta (L.) Lat.) [12], as well as a denitrifying species, A. caeni from activated sludge [18], have been described.…”

Acidovorax valerianellae sp. nov., a novel pathogen of lamb's lettuce [Valerianella locusta (L.) Laterr.]

“…Reference strains included in this study are listed in Table 1. All the strains were grown at 25 uC on YBGA (0?7 % yeast extract, 0?7 % bactopeptone, 0?7 % glucose and 1?5 % agar; pH 7?3) (Gardan et al, 2000).…”