2013
DOI: 10.1159/000346280
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Acidulated Phosphate Fluoride Application Changes the Protein Composition of Human Acquired Enamel Pellicle

Abstract: We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12… Show more

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Cited by 15 publications
(19 citation statements)
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“…Despite the problems inherent in APF gel, it is one of the most used products by dental surgeons in controlling dental caries, demonstrating satisfactory clinical results [32,33]. However, the authors suggest that professionals take more precautions during the application of this product, avoiding contact with ceramic restorations.…”
Section: Discussionmentioning
confidence: 99%
“…Despite the problems inherent in APF gel, it is one of the most used products by dental surgeons in controlling dental caries, demonstrating satisfactory clinical results [32,33]. However, the authors suggest that professionals take more precautions during the application of this product, avoiding contact with ceramic restorations.…”
Section: Discussionmentioning
confidence: 99%
“…1,18,19,[33][34][35] In the present study, the volunteers received two types of meals, taking into consideration the diversity in both foods and quantities that are consumed by children. The definition of quantities and items of menus was made considering the follow-up of diets in a nursery school after each component had been analyzed and weighed.…”
Section: 11mentioning
confidence: 99%
“…É possível que a redução no número de proteínas observada para o esmalte seja devido ao tipo de substrato sobre o qual a película adquirida foi formada (blocos de esmalte humano de 3X3 mm no presente estudo e não dentes naturais, o que pode ter reduzido a área sobre a qual a película foi formada). Em um estudo recente utilizando blocos de esmalte bovino os autores identificaram 56 proteínas diferentes, embora tenham colhido película formada ex vivo a partir de saliva humana e tenham empregado 40 dentes bovinos para a formação da película em cada grupo (MASSON et al, 2013). Outra diferença em relação ao procedimento de coleta da película no presente estudo, quando comparado aos estudos realizados in vivo SIQUEIRA et al, 2010;OPPENHEIM, 2009;SIQUEIRA et al, 2007;ZIMMERMAN et al, 2013), foi o fato de que, no presente estudo, visando reduzir a proteólise das amostras, foi acrescentado um coquetel contendo inibidor de proteases (Complete, Roche Diagnostics) ao ácido cítrico a 3%, no qual os papeis para coleta de película foram imersos.…”
Section: Resultados E Discussâounclassified
“…Vale ressaltar que esta proteína também foi comum a todos os meios de imersão para o esmalte tratado por 10 minutos. Consistente com a literatura, proteínas da família das queratinas vêm sendo consistentemente identificadas na película adquirida MASSON et al, 2013;MCDONALD, 2012;SIQUEIRA et al, 2010;OPPENHEIM, 2009;SIQUEIRA et al, 2007;ZIMMERMAN et al, 2013).…”
Section: Resultados E Discussâounclassified