Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

Abstract

Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consortia were identified as Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae) and Elizabethkingia miricola (E. miricola). The impacts of the critical operating parameters on the elimination of CIP and LEV by bacterial consortia have been investigated and optimized to achieve the maximum levels of CIP and LEV biodegradation. Using liquid chromatography with tandem mass spectrometry (LC-MS-MS), possible degradation pathways for CIP and LEV were suggested by analyzing the intermediate degradation products. The role of the enzymes fluoroquinolone-acetylating aminoglycoside (6′-N-acetyltransferase) and cytochrome P450 (CYP450) in the breakdown of fluoroquinolones (FQs) was investigated as well. According to our findings, various biodegradation mechanisms have been suggested, including cleavage of piperazine ring, substitution of F atom, hydroxylation, decarboxylation, and acetylation, as the main biotransformation reactions. This study discovers the ability of non-reported bacterial strains to biodegrade both CIP and LEV as a sole carbon source, providing new insights into the biodegradation of CIP and LEV. Graphical abstract

The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater

“…Ciprofloxacin and levofloxacin are fluoroquinolone antibiotics that are widely used in hospitals and frequently present in wastewater treatment plants and marine environments. It has been demonstrated that Klebsiella pneumoniae strains obtained from industrial wastewater exhibit biodegradation capabilities toward quinolone drugs [18]. Additionally, newly isolated Klebsiella pneumoniae strains could produce self-secreted biosurfactants that enhance the solubility and bioavailability of xylene, facilitating the biodegradation of hydrophobic and toxic gases [19].…”

Metagenomic analysis reveals diverse microbial community and potential functional roles in Baner rivulet, India

“…Acinetobacter sp., Klebsiella sp., and Elizabethkingia sp. efficiently biodegrade ciprofloxacin and levofloxacin [ 77 ]. Rhizobacter gummiphilus , found at all three sites, participates in the degradation of rubber, a component of tyres and surgical gloves [ 45 ].…”

The effects of emerging contaminants on the behaviour of Acinetobacter calcoaceticus derived from biofilms

“…This phenomenon has been often described as an adaptation to improve stress tolerance since several studies demonstrated that although toxic substances may inhibit microbial growth, the long-term exposure to sub-inhibitory concentrations of a toxic substance may result in an improvement in microbial ability to grow in the presence of these substances or even metabolize them. 57 Shaker et al 58 reported that Acinetobacter baumannii can degrade fluoroquinolones (CIP and LEV). Kim et al 59 demonstrated that the previous adaptation of E. coli to formic acid also resulted in a reduction in the doubling time.…”