Acinetobacter stercoris sp. nov. isolated from output source of a mesophilic german biogas plant with anaerobic operating conditions

Abstract

The Gram-stain-negative, oxidase negative, catalase positive strain KPC-SM-21T, isolated from a digestate of a storage tank of a mesophilic German biogas plant, was investigated by a polyphasic taxonomic approach. Phylogenetic identification based on the nearly full-length 16S rRNA gene revealed highest gene sequence similarity to Acinetobacter baumannii ATCC 19606T (97.0%). Phylogenetic trees calculated based on partial rpoB and gyrB gene sequences showed a distinct clustering of strain KPC-SM-21T with Acinetobacter gerneri DSM 14967T = CIP 107464T and not with A. baumannii, which was also supported in the five housekeeping genes multilocus sequence analysis based phylogeny. Average nucleotide identity values between whole genome sequences of strain KPC-SM-21T and next related type strains supported the novel species status. The DNA G + C content of strain KPC-SM-21T was 37.7 mol%. Whole-cell MALDI-TOF MS analysis supported the distinctness of the strain to type strains of next related Acinetobacter species. Predominant fatty acids were C18:1 ω9c (44.2%), C16:0 (21.7%) and a summed feature comprising C16:1 ω7c and/or iso-C15:0 2-OH (15.3%). Based on the obtained genotypic, phenotypic and chemotaxonomic data we concluded that strain KPC-SM-21T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter stercoris sp. nov. is proposed. The type strain is KPC-SM-21T (= DSM 102168T = LMG 29413T).

Investigating microbial dynamics and potential advantages of anaerobic co-digestion of cheese whey and poultry slaughterhouse wastewaters

“…S4 ). Acinetobacter has been shown to survive under anaerobic conditions when having stored phosphates 64 , 66 . The increase in the relative abundance of Acinetobacter in the CWW reactors over cycles is most likely due to accumulation from the feed.…”

Genomic plasticity and adaptive capacity of the quaternary alkyl-ammonium compound and copper tolerant Acinetobacter bohemicus strain QAC-21b isolated from pig manure

“…All incubations were performed at 25 °C. Temperature dependent growth was tested with the spot assay technique as described by Pulami et al ( 2021 ). Growth in brain heart infusion (BHI) broth (Sigma Aldrich) at 30 °C and 35 °C were performed according to Krizova et al ( 2014 ) and Nemec and Radolfova-Krizova ( 2016 ).…”

“…The whole genome shotgun library was sequenced using the dual index paired-end (v3, 2 × 300 bp) approach for the Illumina MiSeq platform as recommended by the manufacturer (Illumina, San Diego, USA). The genome assembly was performed as described previously (Pulami et al 2021 ). The genome sequence was deposited in NCBI under accession number CAJJDZ000000000.…”

“…Members of the genus Acinetobacter are widespread in nature and have been cultured from both clinical and non-clinical environments including soil, water, wild birds and domestic/farm animals (Towner 2009 ; Visca et al 2011 ; Wilharm et al 2017 ; Pulami et al 2021 ). The ecology of most of the species is still not well understood (Cool et al 2019 ).…”

Imported Pet Reptiles and Their “Blind Passengers”—In-Depth Characterization of 80 Acinetobacter Species Isolates

“…The genus Acinetobacter is highly diverse [ 1 ] and comprises, at the time of writing, approximately 73 validly published species ( (accessed on 8 February 2022)). Members of this genus are Gram-stain negative cocci or cocco-bacilli that are non-motile, non-spore-forming, strictly aerobic, cytochrome-oxidase negative, and catalase-positive bacteria [ 2 ]. Despite one of the genus’ major characteristics of being non-fermentative bacteria, members have been found to survive under different environmental conditions without oxygen for prolonged periods of several weeks.…”