Aclimatación de Agave americana var. Oaxacensis obtenidas in vitro

Arreola, Elvia Yescas; Ángeles, Gisela Virginia Campos; Valle, José Raymundo Enríquez-del; Velasco, Vicente Arturo Velasco; Rodríguez-Ortiz, Gerardo; Luna, Juan Abel Nájera

doi:10.29312/remexca.v7i4.275

Cited by 5 publications

(1 citation statement)

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“…Solución 1= 25% del fertilizante soluble Hydro-Solution; Solución 2= 25% de fertilizante soluble más enraizador Phyto Raizon® Plus (1 g L -1 ). 14 Se tienen antecedentes de la aclimatación exitosa (94-100%) de diversas especies de Agave: A. tequilana (Valenzuela-Sánchez et al, 2006); A. cocui (Salazar, Gonzalez et al, 2009); A. fourcroydes (Abreu et al, 2007); A. americana (Yescas-Arreola et al, 2016). Con los resultados expuestos en la Tabla 7 se puede decir que el proceso de aclimatación fue satisfactorio, ya que las plantas mostraron un reinicio de crecimiento en ambos casos (semi-hidropónico y maceta), sin embargo, existió una merma del 20 % de las plántulas obtenidas de Am transferidas al sistema semihidropónico.…”

Section: Aclimatación De Plántulas a Condición Ex Vitrounclassified

Respuesta Morfogenética De Dos Especies De Agave Regeneradas in Vitro

Fernández

Velasco

Aragón

et al. 2020

Trop Subtrop Agroecosyst

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Background: Agaves in Mexico are plants with great economic, gastronomic and cultural value. Despite the multiple forms of reproduction of plants belonging to the genus Agave, several of these species are threatened due to their slow growth, low percentage of seed viability, destruction of its habitat, excessive looting, interruption of its ripening cycle due to the use of their different organs,however, it has been posible to establish biotechnological techniques for its propagation, conservation and genetic improvement. Objective: At the present work were selected a representative species of Agave, one of them for pulque production (A. salmiana) (As) and the other one in the production of mezcal (Agave marmorata) (Am) with the aim to establish an efficient micropropagation protocol optimizing the concentrations of the citokinin benzylaminopurine (BA) and the auxin 2,4-Dichlorophenoxyacetic acid (2,4-D), on the in vitro morphogenesis of both species. Methodology: Two in vitro culture systems were conducted in three explants (zygotic embryonic axis (E1), in vitro meristematic zone (E2) and ex vitro meristematic zone (E3)). Results: Shoots regeneration was achieved in all tested experiments; the highest number of shoots in As was 23.8 for explant using the E2 of directly regenerated in vitro germinated plants using a concentration of 10 mg L-1 of BA, while in Am 24.7 shoots/explant were obtained with 5 mg L-1 of BA using the zygotic embryonic axis who throughing a callus phase. Implications: The results obtained help to understand the importance of developing specific micropropagation protocols for each species, as well as contribuiting in the masive multiplications of A. salmiana and A. marmorata by understanding the effect of cytokinin BA and auxin 2,4-D and their combination in the formation of shoots by direct and indirect organogenesis. This provides a conservation alternative and an opportunity to startgenetic improvment programs. Conclusion: The results of this research suggest that the choice of explant, multiplication system, species and plant growth regulators are the key to obtain a greater number of shoots with high ex vitro survival rates.

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Section: Aclimatación De Plántulas a Condición Ex Vitrounclassified

Respuesta Morfogenética De Dos Especies De Agave Regeneradas in Vitro

Fernández

Velasco

Aragón

et al. 2020

Trop Subtrop Agroecosyst

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Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Tamayo‐Ordóñez

Ayil‐Gutiérrez

Tamayo-Ordóñez

et al. 2018

Biotechnology Progress

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Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis.

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