Aclimatación ex vitro de plántulas de Fragaria x ananassa Duch.

Juárez, María Concepción Valencia; López, Diana Escobedo; Espino, Luis Febronio Díaz; Pérez, Enrique González

doi:10.29312/remexca.v10i1.1633

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…'Winter Star' en concentración de sales del 50% de MS suplementado con IBA 1,5 mgL -1 se obtuvo mayor número y longitud de raíces en comparación con el medio de cultivo sin reguladores de crecimiento; además indujo la formación de raíces en menor número de días (Mehta et al, 2021). Asimismo, en la variedad 'Nikte' con MS suplementado con 30 gL -1 de sacarosa, 7 gL -1 de agar + 0,2 mgL -1 de BAP, produjo 4,3 raíces por vitroplanta y una longitud de 2,8 cm, superior a otros tratamientos utilizados (Valencia et al, 2019); mientras que, en la variedad 'Chandler', en MS al 50% + 1,0 mgL -1 de IBA se obtuvo 5,8 brotes por vitroplanta y 1,0 mgL -1 de tidiazuron + 2,0 mgL -1 de kinetina no indujo a la formación de raíces (Irshad et al, 2023). En la presente investigación, el medio MS fue sin dilución y que posteriores trabajos se podría disminuir la concentración y así se reduciría el monto de inversión en la micropropagación de estas tres variedades.…”

Section: Multiplicación Y Enraizamientounclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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The objective was to determine the response of three strawberrry varieties (Fragaria x ananassa Duch.) to the aplication of two plant growth regulators: indol acetic acid (IAA) and 6-Benzyl aminopurine (BAP) added to Murashige & Skoog médium in strawberry micropropagation. The complete randomized block design was used and three strawberry varieties ‘Aromas’, ‘Albion’ and ‘Camarosa’ were considered as trials. MS medium for shoot regeneration in initial stage was supplemented with BAP 0.5 mgL-1 and IAA 0.6 mgL-1. Shoot proliferation MS medium was supplemented with BAP 0.5 mgL-1 in multiplication stage and MS medium hormone free was used for rooting induction. It was determined that after 32 days of cultivation the combination of IAA and BAP significantly affected shoot regeneration of apical shoot tips of three strawberry varieties. The greatest effect was caused on ‘Camarosa’. In addition, results on shoot multiplication using BAP indicated that ‘Albion’ performed better in shoot proliferation in terms of shoots per explants reaching 2.51 compared to ‘Camarosa’ and ‘Aromas’ with significant differences. However rooting medium with no growth regulators improves root development with best response of ‘Camarosa’ root length reaching 4.76 cm.

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Section: Multiplicación Y Enraizamientounclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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"EN PRENSA" Medio de cultivo sin autoclave para la micropropagación de un híbrido de Cattleya (Rhyncholaeliocattleya)"EN PRENSA"

Ramírez Mosqueda

2024

rbio

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La importancia ornamental de las orquídeas se debe a las diversas formas, tamaños y colores de sus flores. Desafortunadamente, la propagación asexual y sexual de estas especies lleva mucho tiempo y produce un número limitado de propágulos comerciales. Por tanto, el cultivo de tejidos vegetales ha resultado una alternativa adecuada para la micropropagación. Sin embargo, algunos de los materiales utilizados en esta técnica son caros. Por lo tanto, el objetivo de este estudio fue proponer un método alternativo para la propagación in vitro que involucra el uso de recipientes desechables y un medio de cultivo que no requiere autoclave. Se evaluaron diferentes concentraciones de 6-bencilaminopurina (BAP: 0, 0.5, 1.0, 1.5, 2,0 y 2.5 mg•L-1) en medio MS (Murashige y Skoog) autoclavado y no autoclavado, seguido de aclimatación. Luego de 45 días de incubación, se evaluó el porcentaje de contaminación, número de brotes por explante, longitud de brotes, número de hojas, número de raíces y longitud de raíces. No se observó contaminación utilizando el método alternativo y autoclave. El mayor número de brotes por explante (3.77) se produjo en 1,0 mg•L-1 de BAP en medio de cultivo no autoclavado. Se observó una tasa de supervivencia del 98% durante la fase de aclimatación. Estos resultados pueden utilizarse como una alternativa para la micropropagación comercial de orquídeas.

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In vivo adaptation of regenerant plants of Fragaria vesca L. cultivars

Чорнобров

Tkachova

2020

Plant Varieties Studying and Protection

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The adaptation of regenerant plants to environmental conditions is the final stage of micropropagation. According to a number of authors, when in vitro plants are transferred to in vivo non-sterile conditions, a significant percentage of mortality is recorded. In a previous publication, the regenerative capacity of strawberry (Fragaria vesca L.) in vitro tissues on MS culture medium (Murashige & Skoog, 1962) and a regenerants was obtained (Chornobrov O. Yu., 2019). The objective of the study is to develop an optimal protocol of acclimation of in vitro F. vesca plants to in vivo conditions. Methods. Biotechnological and statistical methods of research were applied. For the research 'Ruiana' and 'Zhovte Dyvo' cultivars were used with in vitro cultivation cycle of 30-35 days. Prepared plants were planted in 0.33 L plastic contai ners, one piece in a mixture of coconut substrate and perlite (3:1). Plants were kept under high relative humidity (85-90%) conditions for 3-5 days, 6-8 days and 10-14 days. The studies were carried out in the Plant Biotechnology Laboratory of SS of NULES of Ukraine "BFRS" during 2019-2020. Results. The duration of Fragaria vesca regenerant plants exposure in conditions of high relative humidity significantly affected adaptation efficiency. The proportion of 'Ruiana' and 'Zhovte Dyvo' plants adapted to the greenhouse conditions were 47.6 ± 2.5% and 60.0 ± 1.7%, respectively, when the plants were kept for 10-14 days. A significant efficiency of plant adaptation (more than 70%) was obtained under condition of preliminarily exposure the roots of the plants in an auxin solution for 25-30 minutes with daily application of 30% solution of glycerine as foliar spray. The plants adapted to the greenhouse conditions had pigmentation characteristic of the variety, without signs of chlorosis and vitrification. Conclusions. An optimal protocol for in vitro adaptation of F. vesca cultivars to in vivo conditions was developed and viable plants were obtained. Further research will be aimed at studying the growth and development of F. vesca regenerant plants in open ground.

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Aclimatación ex vitro de plántulas de Fragaria x ananassa Duch.

Cited by 4 publications

References 13 publications

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

"EN PRENSA" Medio de cultivo sin autoclave para la micropropagación de un híbrido de Cattleya (Rhyncholaeliocattleya)"EN PRENSA"

In vivo adaptation of regenerant plants of Fragaria vesca L. cultivars

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