Acoustic plethysmography measures breathing in unrestrained neonatal mice

Daubenspeck, J. Andrew; Li, Aihua; Nattie, Eugene E.

doi:10.1152/japplphysiol.00893.2007

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Statistical analysis of the results of several recordings showed that the mean values of respiratory parameters in anesthetized mice were reproducible and comparable with values previously reported (Daubenspeck et al, 2008; Hamelmann et al, 1997; Hoymann, 2007; Huang et al, 2011). Several studies reported that the breathing frequency decreases by about 40–60% in anesthetized mice compared with conscious mice (Hamelmann et al, 1997; Takezawa et al, 1980).…”

Section: Discussionsupporting

confidence: 88%

Functional and histopathological identification of the respiratory failure in a DMSXL transgenic mouse model of Myotonic Dystrophy

Panaite¹,

Küntzer²,

Gourdon

et al. 2012

Disease Models &Amp; Mechanisms

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SUMMARYAcute and chronic respiratory failure is one of the major and potentially life-threatening features in individuals with myotonic dystrophy type 1 (DM1). Despite several clinical demonstrations showing respiratory problems in DM1 patients, the mechanisms are still not completely understood. This study was designed to investigate whether the DMSXL transgenic mouse model for DM1 exhibits respiratory disorders and, if so, to identify the pathological changes underlying these respiratory problems. Using pressure plethysmography, we assessed the breathing function in control mice and DMSXL mice generated after large expansions of the CTG repeat in successive generations of DM1 transgenic mice. Statistical analysis of breathing function measurements revealed a significant decrease in the most relevant respiratory parameters in DMSXL mice, indicating impaired respiratory function. Histological and morphometric analysis showed pathological changes in diaphragmatic muscle of DMSXL mice, characterized by an increase in the percentage of type I muscle fibers, the presence of central nuclei, partial denervation of end-plates (EPs) and a significant reduction in their size, shape complexity and density of acetylcholine receptors, all of which reflect a possible breakdown in communication between the diaphragmatic muscles fibers and the nerve terminals. Diaphragm muscle abnormalities were accompanied by an accumulation of mutant DMPK RNA foci in muscle fiber nuclei. Moreover, in DMSXL mice, the unmyelinated phrenic afferents are significantly lower. Also in these mice, significant neuronopathy was not detected in either cervical phrenic motor neurons or brainstem respiratory neurons. Because EPs are involved in the transmission of action potentials and the unmyelinated phrenic afferents exert a modulating influence on the respiratory drive, the pathological alterations affecting these structures might underlie the respiratory impairment detected in DMSXL mice. Understanding mechanisms of respiratory deficiency should guide pharmaceutical and clinical research towards better therapy for the respiratory deficits associated with DM1.

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Section: Discussionsupporting

confidence: 88%

Functional and histopathological identification of the respiratory failure in a DMSXL transgenic mouse model of Myotonic Dystrophy

Panaite¹,

Küntzer²,

Gourdon

et al. 2012

Disease Models &Amp; Mechanisms

View full text Add to dashboard Cite

show abstract

“…; Bissonnette & Knopp, ), a method that directly measures the pressure changes due to inspiration, and they are also consistent with values obtained by acoustic plethysmography in newborn mice (Daubenspeck et al . ). In adult mice, V T can be measured with whole‐body plethysmography with an error <7% of that measured by direct plethymography or pneumotachography (Onodera et al .…”

Section: Discussionmentioning

confidence: 97%

Reduced hypoxic ventilatory response in newborn mice knocked‐out for the progesterone receptor

Potvin

Rossignol

Uppari

et al. 2014

Experimental Physiology

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New Findings r What is the central question of this study?Progesterone has been proposed as an alternative to xanthine therapy for the treatment of idiopathic apnoeas in preterm neonates, but our knowledge of the respiratory function of progesterone in neonates is limited. We therefore asked whether endogenous progesterone is an efficient respiratory stimulant in newborn mice. r What is the main finding and its importance?The present study shows that expression of the nuclear progesterone receptor is a key contributor to the hypoxic ventilatory response in newborn mice. This study will encourage further research on the role of other progesterone receptors in respiratory control in newborn mammals.Recent studies showed that progesterone stimulates the hypoxic ventilatory response and may reduce apnoea frequency in newborn rats, but so far we still do not know by what mechanisms and whether endogenous progesterone might contribute to respiratory control in neonates. We therefore determined the role of the nuclear progesterone receptor (PR; member of the steroid receptor superfamily) by using wild-type (WT) and PR knock-out (PRKO) mice at postnatal days (P) 1, 4 and 10. We measured the hypoxic ventilatory response (14 and 12% O 2 , 20 min each) and apnoea frequency in both male and female mice by using whole-body plethysmography. In response to hypoxia, WT male mice had a marked hypoxic ventilatory response at P1 and P10, but not at P4. At P1 and P10, PRKO male mice had a lower hypoxic ventilatory response than WT males. Wild-type female mice had a marked hypoxic ventilatory response at P10, but not at P1 and P4. At P1 and P10, PRKO female mice had a lower hypoxic ventilatory response than WT females. In basal conditions, apnoea frequency was similar in WT and PRKO mice at P1, P4 and P10. During hypoxia, apnoea frequency was higher in WT male mice compared with PRKO male mice and WT female mice at P1. We conclude that PR is a key contributor to the hypoxic ventilatory response in newborn mice, but PR deletion does not increase the frequency of apnoea during normoxia or hypoxia.

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“…The total volume of the cannula was 130 mL; the tidal volume of a mouse being ~8 mL/g. 13,14 At the time of intubation, the tubing of the cannula was stiffened by insertion of a piece of copper wire via the luer lock adaptor (Figure 2B); the wire being removed immediately after intubation. The cannula, tilted so that the luer lock end was slightly lower (~10 o ) than the tip, was inserted as soon as the vocal cords separated.…”

Section: Resultsmentioning

confidence: 99%

Anesthesia and Intubation of 10-Day Old C57BL/6J Mouse Pups for Cardiothoracic Surgery

Nicks

Skowno

et al. 2021

Preprint

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Murine surgical models play an important role in preclinical research. Mechanistic insights into myocardial regeneration after cardiac injury may be gained from cardiothoracic surgery models in 0-14-day-old mice, the cardiomyocytes of which, unlike those of adults, retain proliferative capacity. Mouse pups up to 7 days old are effectively immobilized by hypothermia and do not require intubation for cardiothoracic surgery. Preadolescent (8-14-day-old) mouse pups, however, do require intubation, but this is challenging and there is little information regarding anesthesia to facilitate intubation. Empirical titration of ketamine/xylazine/atropine dosage regimens to body weight indicated the response to anesthesia of 10-day-old C57BL6/J mouse pups of different weights was non-linear, whereby doses of 20/4/0.12 mg/kg, 30/4/0.12 mg/kg and 50/6/0.18 mg/kg, facilitated intubation of pups weighing between 3.15-4.49 g (n=22), 4.50-5.49 g (n=20) and 5.50-8.10 g (n=20), respectively. Lower-body-weight pups required more intubation attempts than heavier pups (p<0.001). Survival post-intubation was inversely correlated with body weight (65, 70 and 80% for low-, mid- and high-weight groups, respectively, R2=0.995). For myocardial infarction surgery after intubation, a surgical plane of anesthesia was induced with 4.5% isoflurane in 100% oxygen and maintained with 2% isoflurane in 100% oxygen. Survival post-surgery was similar for the three weight groups at 92%, 86% and 88% (p=0.91). Together with refinements in animal handling practices for intubation and surgery, and to minimize cannibalization by the dam post-surgery, overall survival for the entire procedure (intubation plus surgery) was inversely correlated with body weight (55%, 60% and 70% for low-, mid- and high-weight groups, respectively, R2=0.978). Given the difficulty encountered with intubation of 10-day old pups and the associated high mortality, we recommend cardiothoracic surgery in 10-day-old pups be restricted to those weighing at least 5.5 g.

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Acoustic plethysmography measures breathing in unrestrained neonatal mice

Cited by 5 publications

References 12 publications

Functional and histopathological identification of the respiratory failure in a DMSXL transgenic mouse model of Myotonic Dystrophy

Functional and histopathological identification of the respiratory failure in a DMSXL transgenic mouse model of Myotonic Dystrophy

Reduced hypoxic ventilatory response in newborn mice knocked‐out for the progesterone receptor

Anesthesia and Intubation of 10-Day Old C57BL/6J Mouse Pups for Cardiothoracic Surgery

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