The Ponchielli theater of Cremona was built in 1808 after a fire destroyed the old wooden structure. The interior, the architecture and the shape of the plan layout are reminiscent of the Teatro alla Scala, Milan, a masterpiece by the architect Piermarini, albeit on a smaller scale. The four orders of balconies crowned by the top gallery are typical features of a 19th Century Italian Opera theater. Acoustic measurements have been undertaken across the stalls and in some selected boxes according to ISO 3382. The main acoustic parameters resulting from the measurements have been used for the acoustic calibration of a 3D model representing the Ponchielli theater. The calibration has been used to compare different scenarios involving the acoustic response of the main hall at 50% and 100% occupancy. The outcomes indicate that no significant change can be detected when the seats are provided with robust upholstery, which can be considered a positive result, especially for the actors who are not forced to change their effort between rehearsal and live performance. In order to contextualize the measured values in relation to the optimal ones, a comparison with other Italian Opera theaters provided with similar architectural characteristics has been carried out. Overall, the findings indicate that the acoustics of the Ponchielli theater are suitable for both music and speech in line with the other selected theaters, as these places were mainly created for multifunctional purposes in the 19th Century.