Acousto-microfluidics for screening of ssDNA aptamer

Park, Jee-Woong; Lee, Su Jin; Ren, Shuo; Lee, Sangwook; Kim, Soyoun; Laurell, Thomas

doi:10.1038/srep27121

Cited by 38 publications

(28 citation statements)

References 58 publications

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“…More recently, several modified microfluidic techniques have been established to enhance the efficiency in selecting aptamers, including Capillary Electrophoresis (CE) microfluidic SELEX [ 26 ], bead-based microfluidic SELEX [ 27 , 28 ] and protein microarray-microfluidic chip SELEX [ 29 ].…”

Section: Limitations and Advancements In Aptamer Selectionmentioning

confidence: 99%

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Zhang

Wang

et al. 2017

IJMS

359

205

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Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers.

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Section: Limitations and Advancements In Aptamer Selectionmentioning

confidence: 99%

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Zhang

Wang

et al. 2017

IJMS

359

205

View full text Add to dashboard Cite

show abstract

“…While for virus manipulation, Ness et al [43] demonstrate extraction and enrichment of MS2 virus from human nasopharyngeal samples by integration of acoustic force to remove host cells, debris, and pollen from the sample and later with electric field force to attract the virus, which is a negatively charged species, and to migrate from sample to co-flowing buffer. Further, Park et al [44] perform washing and screening of prostate-specific antigen (PSA)-binding aptamer, a single-stranded DNA (ssDNA), using an acoustophoretic separation. The ssDNA pool comprising of ssDNA library is incubated with microbeads which modified with target molecule, before it is introduced to acoustophoretic separation device, where the microbeads with target-bound DNA fragments are focused on central buffer due to acoustophoretic force, while unbound protein and ssDNA are remained in the original buffer at the side flow.…”

Section: Acoustophoretic Manipulation Of Bioparticlesmentioning

confidence: 99%

Biological Particle Control and Separation using Active Forces in Microfluidic Environments

Ali¹,

Kayani²,

Majlis³

2018

Microfluidics and Nanofluidics

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Exploration of active manipulation of bioparticles has been impacted by the development of micro-/nanofluidic technologies, enabling evident observation of particle responses by means of applied tunable external force field, namely, dielectrophoresis (DEP), magnetophoresis (MAG), acoustophoresis (ACT), thermophoresis (THM), and optical tweezing or trapping (OPT). In this chapter, each mechanism is presented in brief yet concise, for broad range of readers, as strong foundation for amateur as well as brainstorming source for experts. The discussion covers the fundamental mechanism that underlying the phenomenon, presenting the theoretical and schematic description; how the response being tuned; and utmost practical, the understanding by specific implementation into bioparticles manipulation engaging from micron-sized material down to molecular level particles.

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“…Augustsson et al reported a microfluidic device to separate phages using antigen (grass pollen allergen; Phl p5)-coated microbeads from commonly used phage display libraries. Park et al developed an acoustofluidic device as a new screening method using a prostate-specific antigen (PSA)-coated microbeads for selection of aptamer based on an acoustofluidic separation (acoustophoresis) technique [28]. In addition, Shields et al demonstrated a new acoustofluidic sorting method using elastomeric affinity particles in order to capture and transport cells into antinode (general channel wall) of an acoustic standing wave [29].…”

Section: Introductionmentioning

confidence: 99%

Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis

Lee¹,

Kim

Shin

et al. 2019

Micromachines

Self Cite

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Here, we report a simple and effective method for capturing and displacement of gram-negative bacteria using aptamer-modified microbeads and acoustophoresis. As acoustophoresis allows for simultaneous washing and size-dependent separation in continuous flow mode, we efficiently obtained gram-negative bacteria that showed high affinity without any additional washing steps. The proposed device has a simple and efficient channel design, utilizing a long, square-shaped microchannel that shows excellent separation performance in terms of the purity, recovery, and concentration factor. Microbeads (10 µm) coated with the GN6 aptamer can specifically bind gram-negative bacteria. After incubation of bacteria culture sample with aptamer affinity bead, gram-negative bacteria-bound microbeads, and other unbound/contaminants can be separated by size with high purity and recovery. The device demonstrated excellent separation performance, with high recovery (up to 98%), high purity (up to 99%), and a high-volume rate (500 µL/min). The acoustophoretic separation performances were conducted using 5 Gram-negative bacteria and 5 Gram-positive bacteria. Thanks to GN6 aptamer’s binding affinity, aptamer affinity bead also showed binding affinity to multiple strains of gram-negative bacteria, but not to gram-positive bacteria. GN6 coated bead can capture Gram-negative bacteria but not Gram-positive bacteria. This study may present a different perspective in the field of early diagnosis in bacterial infectious diseases. In addition to detecting living bacteria or bacteria-derived biomarkers, this protocol can be extended to monitoring the contamination of water resources and may aid quick responses to bioterrorism and pathogenic bacterial infections.

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Acousto-microfluidics for screening of ssDNA aptamer

Cited by 38 publications

References 58 publications

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Biological Particle Control and Separation using Active Forces in Microfluidic Environments

Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis

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