Acoustofluidic enzyme-linked immunosorbent assay (ELISA) platform enabled by coupled acoustic streaming

Li, Xiaojun; Huffman, Justin L.; Ranganathan, Nandhini; He, Ziyi

doi:10.1016/j.aca.2019.05.073

Cited by 26 publications

(16 citation statements)

References 30 publications

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“…Li et al. [41] reported a device for ELISA with a dead‐end side‐channel covered by evenly spaced sharp‐edge structures. These structures were previously utilized in acoustofluidic micromixers, for instance, by Huang et al.…”

Section: Applicationsmentioning

confidence: 99%

“…The asymmetric sharp‐edge structures generate microvortices of acoustic streaming rotating in the direction the edge is pointing to. Microvortices of the neighboring structures combine into a single larger vortex rotating in the whole dead‐end side channel [41].…”

Section: Applicationsmentioning

confidence: 99%

“…An interesting twist of acoustic trapping does not involve the acoustic radiation force but the acoustic streaming instead. Li et al [41] reported a device for ELISA with a dead-end side-channel covered by evenly spaced sharp-edge structures. These structures were previously utilized in acoustofluidic micromixers, for instance, by Huang et al [42], which used symmetrical structures which generated two counter-rotating vortices on each side of their edge to facilitate the mixing.…”

Section: Sorting/trapping Via Acoustic Streamingmentioning

confidence: 99%

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Acoustofluidic platforms for particle manipulation

2021

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There is an increasing interest in acoustics for microfluidic applications. This field, commonly known as acoustofluidics involves the interaction of ultrasonic standing waves with fluids and dispersed microparticles. The combination of microfluidics and the so-called acoustic standing waves (ASWs) led to the development of integrated systems for contactless on-chip cell and particle manipulation where it is possible to move and spatially localize these particles based on the different acoustophysical properties. While it was initially suggested that the acoustic forces could be harmful to the cells and could impact cell viability, proliferation, or function via phenotypic or even genotypic changes, further studies disproved such claims. This review is summarizing some interesting applications of acoustofluidics in the manipulations of biomaterials, such as cells or subcellular vesicles, in works published mainly within the last 5 years.

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Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Section: Sorting/trapping Via Acoustic Streamingmentioning

confidence: 99%

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Acoustofluidic platforms for particle manipulation

2021

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“…If the particles are smaller than 6 µm, the assembling strategy mentioned here would become less effective owing to the weakened radiation force. Under this condition, the acoustic streaming around oscillated microstructures would dominate micro or nano particles' movement, which have been proven to realize the controllable manipulation, such as rotating cells and organisms [31], microbead-based enhancement of molecular exchange [32], and bi-directional transportation of micro-agents [33], among others.…”

Section: Chain-like Assembling Of Micro Particlesmentioning

confidence: 99%

Local Acoustic Fields Powered Assembly of Microparticles and Applications

et al. 2019

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Controllable assembly in nano-/microscale holds considerable promise for bioengineering, intracellular manipulation, diagnostic sensing, and biomedical applications. However, up to now, micro-/nanoscopic assembly methods are severely limited by the fabrication materials, as well as energy sources to achieve the effective propulsion. In particular, reproductive manipulation and customized structure is quite essential for assemblies to accomplish a variety of on-demand tasks at small scales. Here, we present an attractive assembly strategy to collect microparticles, based on local acoustic forces nearby microstructures. The micro-manipulation chip is built based on an enhanced acoustic field, which could tightly trap microparticles to the boundaries of the microstructure by tuning the applied driving frequency and voltage. Numerical simulations and experimental demonstrations illustrate that the capturing and assembly of microparticles is closely related to the size of particles, owing to the vibration-induced locally enhanced acoustic field and resultant propulsion force. This acoustic assembly strategy can open extensive opportunities for lab-on-chip systems, microfactories, and micro-manipulators, among others.

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“…In the past 3 decades, miniaturization of analytical instruments and devices has been rapidly advanced by the progress of microfluidics and microfabrication technology. , Implementing MB-based ELISAs in lab-on-a-chip systems instead of microwell plates can significantly boost the assay preference, − including reduction of the consumption of MBs and samples, , cutting down the reaction period, , and improvement in assay sensitivity. , According to critical reviews, this improvement in performance attributes to both the reduction in the diffusion mass transfer resistance across the microchannel cross section and the effective washing of MBs in the microfluidic channel . Moreover, Barbosa and co-workers found that the activity of horseradish peroxidase (HRP) and the efficiency of the enzymatic reaction can be enhanced in the microchannel .…”

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confidence: 99%

Microfluidic Magnetic Analyte Delivery Technique for Separation, Enrichment, and Fluorescence Detection of Ultratrace Biomarkers

et al. 2021

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A microfluidic magnetic analyte delivery (μMAD) technique was developed to realize sample preparation and ultrasensitive biomarker detection. A simply designed microfluidic device was employed to carry out this technique, including a poly(dimethylsiloxane)-glass hybrid microchip having four straight rectangular channels and a permanent magnet. In the μMAD process, functionalized magnetic beads (MBs) were used to recognize and isolate analytes from a complex sample matrix, deliver analytes into tiny microchannels, and preconcentrate analytes in the magnetic trapping/detection region for in situ fluorescence detection. In the feasibility study and sensitivity optimization, horseradish peroxidase-labeled MBs were used, and critical parameters for the signal amplification performance of μMAD were carefully evaluated. At optimized conditions, a sensitivity improvement of at least 2 orders of magnitude was achieved. As a proof of concept, μMAD was combined with the enzyme-linked immunosorbent assay (ELISA), while carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), and interleukin 6 (IL-6) were selected as model biomarkers. The limits of detection (LODs) of μMAD-ELISA were as low as 0.29 pg/mL for CEA, 0.047 pg/mL for PSA, and 0.021 pg/mL for IL-6, which corresponded to an over 200-fold reduction compared to their commercial ELISA results. Meanwhile, μMAD-ELISA revealed high selectivity and reproducibility. In clinical sample analysis, good accuracy was acquired for human serum analysis relative to commercial ELISA kits, and satisfied recoveries of 85.1−102% with RSDs of 1.7−9.8% for IL-6 and 84.7−113% with RSDs of 3.2−8.3% for interferon-γ were obtained. This ultrasensitive and easy operation technique provides a valuable approach for trace-level biomarker detection for practical applications.

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Acoustofluidic enzyme-linked immunosorbent assay (ELISA) platform enabled by coupled acoustic streaming

Cited by 26 publications

References 30 publications

Acoustofluidic platforms for particle manipulation

Acoustofluidic platforms for particle manipulation

Local Acoustic Fields Powered Assembly of Microparticles and Applications

Microfluidic Magnetic Analyte Delivery Technique for Separation, Enrichment, and Fluorescence Detection of Ultratrace Biomarkers

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