2016
DOI: 10.1063/1.4954744
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Acoustofluidic harvesting of microalgae on a single chip

Abstract: We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting o… Show more

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Cited by 15 publications
(10 citation statements)
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“…To date, diverse microfluidic platforms have been proposed in connection with microalgal biotechnology applications: e.g., pixel-based photo-bioreactor [ 6 ], on-chip hydrothermal liquefaction [ 7 ], toxicity screening [ 8 ], and bio-solar cell [ 9 ]. Besides, attempts to separate microalgae using this microfluidic technology are also recently being made [ 10 , 11 ]. For example, Wang et al designed a two-stage microfluidic chip, which could separate the microalgae by their sizes and dielectric properties [ 10 ], and Park et al presented an acoustofluidic platform, which could obtain a target microalgal species from a heterogeneous population of cells [ 11 ].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…To date, diverse microfluidic platforms have been proposed in connection with microalgal biotechnology applications: e.g., pixel-based photo-bioreactor [ 6 ], on-chip hydrothermal liquefaction [ 7 ], toxicity screening [ 8 ], and bio-solar cell [ 9 ]. Besides, attempts to separate microalgae using this microfluidic technology are also recently being made [ 10 , 11 ]. For example, Wang et al designed a two-stage microfluidic chip, which could separate the microalgae by their sizes and dielectric properties [ 10 ], and Park et al presented an acoustofluidic platform, which could obtain a target microalgal species from a heterogeneous population of cells [ 11 ].…”
Section: Introductionmentioning
confidence: 99%
“…Besides, attempts to separate microalgae using this microfluidic technology are also recently being made [ 10 , 11 ]. For example, Wang et al designed a two-stage microfluidic chip, which could separate the microalgae by their sizes and dielectric properties [ 10 ], and Park et al presented an acoustofluidic platform, which could obtain a target microalgal species from a heterogeneous population of cells [ 11 ]. Although these previous studies were able to achieve the separation of microalgae, their dependence on external force fields limits the accessibility of the end-users without expertise in chip technology.…”
Section: Introductionmentioning
confidence: 99%
“…1 A (see SI Appendix , Fig. S1, for a complete schematic), our SRS imaging flow cytometer consists of 1) pulse sources that generate the trains of picosecond pump laser pulses and subharmonically synchronized, pulse pair-resolved, wavelength-switched Stokes laser pulses for the acquisition of 4-color SRS signals; 2) a microfluidic chip (28) with a piezoelectric transducer for acoustic 3D focusing (29, 30) of fast-flowing cells; 3) focusing optics with a resonant galvanometric scanner for scanning the focal spot of the laser pulses in the direction perpendicular to the cell flow for 2D SRS imaging; and 4) a photodetector with a lock-in detection circuit for detecting the SRS signal by demodulating the pump intensity modulated by the SRS process. The cell flow is pumped by a syringe pump at a volume rate of ∼24 µL/min to produce a constant single stream of cells at a flow speed of 2 cm/s at the center of the microchannel.…”
Section: Resultsmentioning
confidence: 99%
“…The development of micro-size particle manipulation technology, it will replace the original traditional micro-size particle sorting technology such as centrifugation and filtration. The main existing techniques for sorting micro-sized particles are dielectrophoretic sorting [1][2][3], acoustic sorting [4], magnetophoretic sorting [5], and inertial flow sorting [6]. Dielectrophoresis sorting does not need to mark the particles before manipulation [7], and there is no contact with the particles during the manipulation process, simple equipment, flexible manipulation, and has the advantages of more minor damage to biological particles [8].…”
Section: Introductionmentioning
confidence: 99%