Acoustofluidic separation of cells and particles

Wu, Mengxi; Özçelik, Adem; Rufo, Joseph; Wang, Zeyu; Fang, Ruiming; Huang, Tony Jun

doi:10.1038/s41378-019-0064-3

Cited by 326 publications

(229 citation statements)

References 149 publications

(167 reference statements)

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“…where the upper bar represents a time-averaged value over a full oscillation time period. For the steady second-order acoustic streaming field, ∂ρ 2 ∂t = 0 and ∂u 2 ∂t = 0, Equations (8) and (9) can be simplified as:…”

Section: Theory and Simulationmentioning

confidence: 99%

“…Contactless manipulation of micro-/nano-scale particles and biosamples in a microfluidic chamber is of great importance [1][2][3] due to the increasing requirement to carry out elementary operations like trapping [4,5], rotation [6,7], separation [8], and concentration [9], which are an essential technique for biological researches and exhibit numerous commercial applications in the bioengineering and pharmaceutical industries [10][11][12]. Various microfluidic manipulation methods for capturing [13,14], transporting [15], rotating [16], and separating [17] of micro-/nano-scale objects have been provided and verified in laboratories worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of 2D Acoustofluidic Fields in an Ultrasonic Cavity Generated by Multiple Vibration Sources

et al. 2019

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Two-dimensional acoustofluidic fields in an ultrasonic chamber actuated by segmented ring-shaped vibration sources with different excitation phases are simulated by COMSOL Multiphysics. Diverse acoustic streaming patterns, including aggregation and rotational modes, can be feasibly generated by the excitation of several sessile ultrasonic sources which only vibrate along radial direction. Numerical simulation of particle trajectory driven by acoustic radiation force and streaming-induced drag force also demonstrates that micro-scale particles suspended in the acoustofluidic chamber can be trapped in the velocity potential well of fluid flow or can rotate around the cavity center with the circumferential acoustic streaming field. Preliminary investigation of simple Russian doll- or Matryoshka-type configurations (double-layer vibration sources) provide a novel method of multifarious structure design in future researches on the combination of phononic crystals and acoustic streaming fields. The implementation of multiple segmented ring-shaped vibration sources offers flexibility for the control of acoustic streaming fields in microfluidic devices for various applications. We believe that this kind of acoustofluidic design is expected to be a promising tool for the investigation of rapid microfluidic mixing on a chip and contactless rotational manipulation of biosamples, such as cells or nematodes.

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Section: Theory and Simulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of 2D Acoustofluidic Fields in an Ultrasonic Cavity Generated by Multiple Vibration Sources

et al. 2019

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“…Ultrasonic particle manipulation (UMP) is a contactless method that is well-suited for micromanipulation in microfluidic systems. Most UPM devices that are shown in literature use standing waves to manipulate particles for applications, such as patterning [1][2][3][4], focusing [5][6][7], and separation [8][9][10] of microparticles. When a standing wave field is established in a microfluidic channel, the movements of particles suspended in the fluid medium are determined by two main forces, i.e., the acoustic radiation force (ARF) and the acoustic streaming (AS) induced drag force, which scale with the volume and diameter of the particle, respectively.…”

Section: Introductionmentioning

confidence: 99%

Numerical Simulation of Boundary-Driven Acoustic Streaming in Microfluidic Channels with Circular Cross-Sections

Lei

Cheng

2020

Micromachines

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While acoustic streaming patterns in microfluidic channels with rectangular cross-sections have been widely shown in the literature, boundary-driven streaming fields in non-rectangular channels have not been well studied. In this paper, a two-dimensional numerical model was developed to simulate the boundary-driven streaming fields on cross-sections of cylindrical fluid channels. Firstly, the linear acoustic pressure fields at the resonant frequencies were solved from the Helmholtz equation. Subsequently, the outer boundary-driven streaming fields in the bulk of fluid were modelled while using Nyborg’s limiting velocity method, of which the limiting velocity equations were extended to be applicable for cylindrical surfaces in this work. In particular, acoustic streaming fields in the primary (1, 0) mode were presented. The results are expected to be valuable to the study of basic physical aspects of microparticle acoustophoresis in microfluidic channels with circular cross-sections and the design of acoustofluidic devices for micromanipulation.

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“…The use of a large amount of sheath fluid also significantly increases the operating cost. Other approaches mainly rely on the internally or externally imposed forces, such as inertial,[10] viscoelastic [11], electric [12], acoustic [13], and hybrid forces [14,15]. These approaches can accomplish good separation in many circumstances but may have potential drawbacks when the biological particles are handled.…”

Section: Introductionmentioning

confidence: 99%

Separation of micro and sub‐micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis

Xue

Sun

et al. 2020

Electrophoresis

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In the present study, we numerically demonstrate an approach for separation of micro and sub-micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis. The dual streams are constructed by an intermediate sheath flow, after which the negative magnetophoretic force induced by an array of permanent magnets dominates the separation of diamagnetic particles. A simple and efficient numerical model is developed to calculate the motions of particles under the action of magnetic field and flow field. Effects of the average flow velocity, the ratio of sheath fluid flow to sample fluid flow, the number of the magnet pair as well as the position of magnet pair are investigated. The optimal parametric condition for complete separation is obtained through the parametric analysis, and the separation principle is further elucidated by the force analysis. The separation of smaller micro and sub-micro diamagnetic particles is finally demonstrated. This study provides an insight into the negative magnetophoretic phenomenon and guides the fabrication of feasible, low-cost diagnostic devices for sub-micro particle separation. Abbreviations: SE, separation efficiencyColor online: See article online to view Figs. 1-4 in color.

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Acoustofluidic separation of cells and particles

Cited by 326 publications

References 149 publications

Diversity of 2D Acoustofluidic Fields in an Ultrasonic Cavity Generated by Multiple Vibration Sources

Diversity of 2D Acoustofluidic Fields in an Ultrasonic Cavity Generated by Multiple Vibration Sources

Numerical Simulation of Boundary-Driven Acoustic Streaming in Microfluidic Channels with Circular Cross-Sections

Separation of micro and sub‐micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis

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