Acoustophoretic cell and particle trapping on microfluidic sharp edges

Leibacher, Ivo; Hahn, Philipp; Dual, Jürg

doi:10.1007/s10404-015-1621-1

Cited by 67 publications

(49 citation statements)

References 54 publications

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“…Typically, a bead with a diameter d p flowing in an acoustofluidic device experiences two forces arising from the flow hydrodynamics and the acoustic field . The drag force F D , caused by the microstreaming flow, is directly proportional to the bead diameter, i.e., F D ∝ d p , while the acoustic radiation force F R , due to the scattering of the incident waves from the oscillating cell, is related to the bead diameter as

F_{R} \propto d_{p}^{3}

. These relationships imply that the drag force is the dominant force for a smaller bead (e.g., d p = 1 µm).…”

mentioning

confidence: 99%

“…[5] The drag force F D , caused by the microstreaming flow, is directly proportional to the bead diameter, i.e., F D ∝ d p , while the acoustic radiation force F R , due to the scattering of the incident waves from the oscillating cell, is related to the bead dia meter as ∝ R p 3 F d . [5,26] These relationships imply that the drag force is the dominant force for a smaller bead (e.g., d p = 1 µm). In this regime, the bead acts as a flow tracer, as shown in Figure 2a-c.…”

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confidence: 99%

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Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Salari

Appak-Baskoy

Ezzo

et al. 2019

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The interaction of a sound or ultrasound wave with an elastic object, such as a microbubble, can give rise to a steady‐state microstreaming flow in its surrounding liquid. Many microfluidic strategies for cell and particle manipulation, and analyte mixing, are based on this type of flow. In addition, there are reports that acoustic streaming can be generated in biological systems, for instance, in a mammalian inner ear. Here, new observations are reported that individual cells are able to induce microstreaming flow, when they are excited by controlled acoustic waves in vitro. Single adherent cells are exposed to an acoustic field inside a microfluidic device. The cell‐induced microstreaming is then investigated by monitoring flow tracers around the cell, while the structure and extracellular environment of the cell are altered using different chemicals. The observations suggest that the maximum streaming flow induced by an MDA‐MB‐231 breast cancer cell can reach velocities on the order of mm s−1, and this maximum velocity is primarily governed by the overall cell stiffness. Therefore, such cell‐induced microstreaming measurements, including flow pattern and velocity magnitude, may be used as label‐free proxies of cellular mechanical properties, such as stiffness.

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F_{R} \propto d_{p}^{3}

. These relationships imply that the drag force is the dominant force for a smaller bead (e.g., d p = 1 µm).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Salari

Appak-Baskoy

Ezzo

et al. 2019

Small

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“…Acoustics provide a noninvasive and robust transduction mechanism that has been widely used in micromixing, micropumping, particle or cell separation, and manipulation, protein crystal patterning, microfluidic switches, droplet production, drug delivery, and actuation of micro‐ and nanoswimmers . Acoustic wave–induced microvortices around solid structures and bubbles have been used to perform manipulation of small objects .…”

Section: Introductionmentioning

confidence: 99%

“…Acoustics provide a noninvasive and robust transduction mechanism that has been widely used in micromixing, [6][7][8] micropumping, [9] particle or cell separation, [10,11] and manipulation, [12][13][14][15] protein crystal patterning, [16] microfluidic…”

Section: Introductionmentioning

confidence: 99%

3D Manipulation and Imaging of Plant Cells using Acoustically Activated Microbubbles

et al. 2019

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The precise manipulation of single cells and organisms opens exciting new possibilities for biological research. In this work, an acoustic rotational manipulation method for imaging single cells of different plant species (pollen grains of Lilium longiflorum and Arabidopsis thaliana) is demonstrated. Acoustically activated microbubbles generate radiation forces as well as microvortices in the aqueous medium, which allow various specimens to be trapped and precisely rotated. The rotational behavior of individual plant cells is studied and their motion to facilitate 3D fluorescent microscopy is controlled. The use of this manipulation technique for high‐resolution 3D optical reconstructions of nontransparent samples is demonstrated. The applicability of this method for open‐microchannel arrangement, which may enable multiplexed 3D access to samples for microsurgery and injection, is further demonstrated.

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“…Distortions of the channel wall cause the acoustic wave to refract at unexpected angles. 42 Each layer contains at least two sets of alignment marks consisting of an asymmetrical pattern of crosses, 43 enabling different layers to be aligned precisely on top of each other. The masks for the individual microfluidic device layers are ordered from CAD/Art Services, Inc. (Bandon, OR) and imaged with a resolution of 25 400 dpi.…”

Section: Device Designmentioning

confidence: 99%

Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design

et al. 2017

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We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.

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Acoustophoretic cell and particle trapping on microfluidic sharp edges

Cited by 67 publications

References 54 publications

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

3D Manipulation and Imaging of Plant Cells using Acoustically Activated Microbubbles

Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design

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