Acquired resistance to monepantel in<i>C. elegans</i>: What about parasitic nematodes?

Fru, Michael Forbiteh; Puoti, Alessandro

doi:10.4161/21624046.2014.959416

Cited by 5 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike broad-spectrum nematocides such as ivermectin, aldicarb and monepantal that are administered in liquid or on solid media (Kampfe and Schutze, 1995;Goldman et al, 2007;Donnelly et al, 2013;Fru and Puoti, 2014;Wever et al, 2015), ZTDO is a synthetic version of the host Oriental beetle pheromone that exerts lethality as a volatile compound on P. pacificus embryos. We have uncovered a striking example of an odor that can arrest nematode embryos through the eggshell commonly perceived to be impermeable to large molecules.…”

Section: Discussionmentioning

confidence: 99%

A species-specific nematocide that results in terminal embryogenesis

Renahan

Hong

2017

Journal of Experimental Biology

View full text Add to dashboard Cite

Nematode-insect interactions are ubiquitous, complex and constantly changing as the host and nematode coevolve. The entomophilic nematode is found on a myriad beetle species worldwide, although the molecular dynamics of this relationship are largely unknown. To better understand how host cues affect embryogenesis, we characterized the threshold of sensitivity to the pheromone ()-7-tetradecen-2-one (ZTDO) by determining the minimum exposure duration and developmental window that results in embryonic lethality. We found early-stage embryos exposed to volatile ZTDO for as few as 4 h all display terminal embryogenesis, characterized by punctuated development up to 48 h later, with abnormal morphology and limited cavity formation. To determine if the pheromone arrests pre-hatching development by suffocating or permeabilizing the eggshells, we raised embryos under anoxic conditions and also examined eggshell permeability using the lipophilic dye FM4-64. We found that asphyxiating the embryos arrested embryogenesis in a reversible manner but did not phenocopy the effects of ZTDO exposure, whereas the ZTDO-induced disruption of embryogenesis did correlate with increased eggshell permeability. The effects of ZTDO are also highly specific, as other lipid insect compounds do not produce any detectable embryocidal effect. The high specificity and unusual teratogenic effect of ZTDO may be important in mediating the host-nematode relationship by regulating development.

show abstract

Section: Discussionmentioning

confidence: 99%

A species-specific nematocide that results in terminal embryogenesis

Renahan

Hong

2017

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

In vitro efficacy of anthelmintics on Angiostrongylus cantonensis L3 larvae

et al. 2020

View full text Add to dashboard Cite

Angiostrongylus cantonensis is the leading cause of eosinophilic meningitis worldwide, with life-threatening complications if not managed correctly. Previous in vitro studies have utilized change in motility patterns of adult female worms to assess the efficacy of anthelmintics qualitatively. However, it is the third stage larvae (L3) that are infectious to humans. With differential staining using propidium iodide penetration as the indicator of death, we can distinguish between dead and live larvae. This assay has enabled us to quantify the in vitro efficacy of nine clinically established anthelmintics on A. cantonensis L3. All drugs were tested at a 1 mm concentration. Piperazine and niclosamide were ineffective in inducing larval death; however, albendazole sulfoxide, pyrantel pamoate, diethylcarbamazine, levamisole and praziquantel were effective as compared to unexposed controls (P < 0.05). Ivermectin and moxidectin did not induce significant levels of mortality, but they considerably reduced larval motility almost immediately. This study indicates the need for further in vivo studies to determine the optimal dose and time frame for post-infection treatment with anthelmintics that demonstrated efficacy.

show abstract

A Species-Specific Nematocide that Results in Terminal Embryogenesis

Hong

Renahan

2017

Preprint

View full text Add to dashboard Cite

Summary StatementInsect-associated nematodes coordinate their development using host cues to "walk the line" between mutualism and pathogenesis. A host pheromone causes terminal embryogenesis by permeablizing the nematode eggshell.peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission.The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/118141 doi: bioRxiv preprint first posted online Mar. 18, 2017; Abstract 1 Nematode-insect interactions are ubiquitous, complex, and constantly changing 2 as the host and nematode coevolve. The entomophilic nematode Pristionchus 3 pacificus is found on a myriad of beetle species worldwide, though the molecular 4 dynamics of this relationship are largely unknown. To better understand how host 5 cues affect P. pacificus embryogenesis, we characterized the threshold of 6 sensitivity to the pheromone (Z)-7-tetradecen-2-one (ZTDO) by determining the 7 minimum exposure duration and developmental window that results in P. 8 pacificus embryonic lethality. We found early-stage embryos exposed to volatile 9 ZTDO for as few as four hours all display terminal embryogenesis, characterized 10 by punctuated development up to 48 hours later, with abnormal morphology and 11 limited lumen formation. To determine if the pheromone arrests pre-hatching 12 development by suffocating or permeabilizing the eggshells, we raised embryos 13 under anoxic condition as well as examined eggshell permeability using the 14 lipophilic dye FM4-64. We found that asphyxiating the embryos arrested 15 embryogenesis in a reversible manner but did not phenocopy the effects of 16 ZTDO exposure, whereas the ZTDO-induced disruption of embryogenesis did 17 correlate with increased eggshell permeability. The effects of ZTDO are also 18 highly specific, as other lipid insect compounds do not produce any detectable 19 embryocidal effect. The high specificity and unusual teratogenic effect of ZTDO 20 may be important in mediating the host-nematode relationship by regulating P. 21 pacificus development. 22 23 peer-reviewed)

show abstract

Acquired resistance to monepantel inC. elegans: What about parasitic nematodes?

Cited by 5 publications

References 18 publications

A species-specific nematocide that results in terminal embryogenesis

A species-specific nematocide that results in terminal embryogenesis

In vitro efficacy of anthelmintics on Angiostrongylus cantonensis L3 larvae

A Species-Specific Nematocide that Results in Terminal Embryogenesis

Contact Info

Product

Resources

About