Acquired sulphonamide resistance genes in faecal Escherichia coli from healthy children in Bolivia and Peru

Infante, Berónica; Grape, Malin; Larsson, Mattias; Kristiansson, Charlotte; Pallecchi, Lucia; Rossolini, Gian María; Kronvall, Göran

doi:10.1016/j.ijantimicag.2004.12.004

Cited by 42 publications

(37 citation statements)

References 21 publications

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“…These findings suggest either that class I integrons in our isolates have lost the sul1 gene region or that this gene is carried on another genetic context in these strains. Similar findings have been reported by others Infante et al, 2005;Kerrn et al, 2002). Furthermore, we also found that about 13 % of isolates that were resistant to sulphafurazole did not harbour any sul alleles.…”

Section: Discussionsupporting

confidence: 92%

“…In our study, we used this set of primers to identify the functionality of our strains to mobilize the resistance gene, and we detected the presence of both functional and nonfunctional proteins encoded by our PCR products. Whilst sequencing of these PCR products (309 bp amplicon for functional and 308 bp amplicon for non-functional) did not fully confirm the presence or absence of functional Although the pattern of gene-frequency distribution in most published data is sul2.sul1.sul3 (Blahna et al, 2006;Grape et al, 2003;Infante et al, 2005;Kerrn et al, 2002), our results indicated that this pattern for UPEC isolates in Queensland, Australia, was sul1.sul2.sul3. The relationship between integron class I and the sulphonamide-resistance gene (sul1) as a conserved segment of an integron class I component has frequently been pointed out before (Ho et al, 2009;Kerrn et al, 2002;Sköld, 2000).…”

Section: Discussioncontrasting

confidence: 76%

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Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia

Gündoğdu

Long

Vollmerhausen

et al. 2011

Journal of Medical Microbiology

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We studied 137 uropathogenic Escherichia coli (UPEC) isolates from hospitalized adult patients (Queensland, Australia) for their resistance to 17 antimicrobial agents using the calibrated dichotomous sensitivity method and the presence of class I, II and III integron-associated integrase (intI) genes, including functional class II intI2, as well as the presence of sul1, sul2 and sul3 genes, using PCR. Randomly amplified polymorphic DNA PCR, a high-resolution biochemical-fingerprinting method (PhP) and phylogenetic grouping were also used to identify the clonality of the sulphonamide-resistant isolates. One hundred and twenty (87.6 %) isolates were resistant to one or more of the tested antimicrobial drugs, with the highest resistance (70.1 %) observed against sulphafurazole (96 isolates). Of these, 84 (87.5 %) contained one or more sul alleles, with sul1 being the most common allele [occurring in 69 (72 %) isolates]. Only 38 of 69 (55.1 %) strains carrying the sul1 gene were positive for class I integrase. Our results indicate a high prevalence of sulphafurazole-resistant UPEC strains belonging to different clones among patients with urinary tract infection in Queensland, Australia. We also conclude that these strains carry predominantly a sul1 gene that is not commonly associated with the presence of class I integrase, indicating that it may be carried on either a bacterial chromosome or other genetic elements. INTRODUCTIONUrinary tract infection (UTI), although one of the most easily treatable diseases, has been reported to be the most common hospital-acquired infection, affecting mainly women, children and the elderly (Foxman & Brown, 2003;Gupta et al., 2001;Russo & Johnson, 2003;Tartof et al., 2007). Escherichia coli is the primary aetiological agent, accounting for 75-90 % cases of UTI (Gupta et al., 2001;Kaper et al., 2004;Nicolle, 2002). The acquisition of resistance genes by horizontal transfer is currently thought to play a major role in the development of multi-drugresistant (MDR) strains because a substantial proportion of the resistance genes are located on conjugative plasmids, transposons, insertion sequences and integrons (Chang et al., 2011;Fierer & Guiney, 1999;Moritz & Hergenrother, 2006). Integrons are mobile DNA elements with the ability to integrate and express gene cassettes by site-specific recombination (Mazel, 2006;Rowe-Magnus & Mazel, 2002). Site-specific recombination is catalysed by an integrase that is encoded within the conserved 59 region of integrons. Several classes of integrase have been described with those classes of integrons, i.e. class I integrase (intI1) defines class I integrons (Collis et al., 2002;Nield et al., 2001).With the standard treatment for UTI being a combination of trimethoprim and sulphamethoxazole (Grape et al., 2003), the sulphonamides are classified as a highly important antimicrobial agent for treatment of E. coli infections, and the presence of sulphonamide resistance can lead to treatment failure in cases of UTI. In E. coli, sulphonamide resistance ofte...

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 76%

Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia

Gündoğdu

Long

Vollmerhausen

et al. 2011

Journal of Medical Microbiology

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“…Based on the PCR results, the sul2 gene has the highest prevalence in the examined co-trimoxazole resistant E. coli strains. Frequency of sul2 (73%) was higher than that of sul1 (31%) and sul3 (4%), which is in accordance with other studies conducted by Grape et al (2003), Infante et al (2005) and Wu et al (2010).…”

Section: Discussionsupporting

confidence: 92%

“…This study assessed the presence of int1 gene among the studied isolates, and detected the gene in 28 (62%) of them. These results approximates the finding of Infante et al (2005), who found int1 gene in 9 (45%) of their studied 20 isolates and Lavakhamseh et al (2016) who recorded the presence of the same gene in 47% of their studied isolates. On the other hand, higher percentage of int1 gene (95%) was recorded by Frank et al (2007).…”

Section: Discussionsupporting

confidence: 90%

Resistance genes to sulphonamide in commensal Escherichia coli isolated from stool of patients in Mansoura University Children Hospital

El-Kazzaz¹,

Mashaly²,

El-Sabbagh³

et al. 2016

Afr. J. Microbiol. Res.

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“…These observations were consistent with contamination of hides with COT r E. coli in lairage since the occurrence of lot 1 fecal samples with COT r E. coli concentrations Ն3.00 log CFU/ swab fell from 41.9 to 24.3% from feedlot to processing (Tables 1 and 3). (86,87,89,90). However, the pattern sul1 Ͼ sul2 Ͼ sul3 has been observed in at least one study of human COT r E. coli isolates (88).…”

Section: Discussionmentioning

confidence: 97%

Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum

Schmidt

Agga

Bosilevac

et al. 2015

Appl Environ Microbiol

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Specific concerns have been raised that third-generation cephalosporin-resistant (3GC r ) Escherichia coli, trimethoprim-sulfamethoxazole-resistant (COT r ) E. coli, 3GC r Salmonella enterica, and nalidixic acid-resistant (NAL r ) S. enterica may be present in cattle production environments, persist through beef processing, and contaminate final products. The prevalences and concentrations of these organisms were determined in feces and hides (at feedlot and processing plant), pre-evisceration carcasses, and final carcasses from three lots of fed cattle (n ‫؍‬ 184). The prevalences and concentrations were further determined for strip loins from 103 of the carcasses. 3GC r Salmonella was detected on 7.6% of hides during processing and was not detected on the final carcasses or strip loins. NAL r S. enterica was detected on only one hide. 3GC r E. coli and COT r E. coli were detected on 100.0% of hides during processing. Concentrations of 3GC r E. coli and COT r E. coli on hides were correlated with pre-evisceration carcass contamination. 3GC r E. coli and COT r E. coli were each detected on only 0.5% of final carcasses and were not detected on strip loins. Five hundred and 42 isolates were screened for extraintestinal pathogenic E. coli (ExPEC) virulence-associated markers. Only two COT r E. coli isolates from hides were ExPEC, indicating that fed cattle products are not a significant source of ExPEC causing human urinary tract infections. The very low prevalences of these organisms on final carcasses and their absence on strip loins demonstrate that current sanitary dressing procedures and processing interventions are effective against antimicrobial-resistant bacteria.

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Acquired sulphonamide resistance genes in faecal Escherichia coli from healthy children in Bolivia and Peru

Cited by 42 publications

References 21 publications

Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia

Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia

Resistance genes to sulphonamide in commensal Escherichia coli isolated from stool of patients in Mansoura University Children Hospital

Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum

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