Acquired Tetracycline Resistance Genes

Roberts, Marilyn C.

doi:10.1007/978-1-4614-1400-1_16

Cited by 19 publications

(23 citation statements)

References 89 publications

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“…The peisistence of tetC during both manure and wastewater treatments was probably due to its frequent association with plasmids, which promoted its transfer between different bacteria (Roberts, 2012). The relative abundances of SRGs decreased from 1.36 × 10 −2 -1.86 × 10 −1 to 1.76 × 10 −4 -5.51 × 10 −2 along the wastewater treatment path, but were more abundant than most TRGs (Fig.…”

Section: −2mentioning

confidence: 95%

“…3). tetE is often associated with non-conjugative plasmids, which may restrict its dissemination (Ng et al, 2001); tetK is usually related to methicillinresistant Staphylococcus aureus (MRSA), a multidrug resistant pathogen (Roberts, 2012). Thus, it is not surprising for their absence.…”

Section: Detection Frequencies Of Argsmentioning

confidence: 99%

“…Among the three main mechanisms responsible for tetracycline resistance, the EFP requires proton motive force to expel TCs out of bacterial membranes (Roberts, 2012); the enzymatic inactivation (tetX) needs oxygen and nicotinamide adenine dinucleotide phosphate to inactivate TCs (Yang et al, 2004); and the RPP needs guanosine triphosphate to trigger the release of TCs from their target sites . All the three tetracycline resistance mechanisms need to consume energy.…”

Section: Correlation Analysismentioning

confidence: 99%

“…They may assist EFP TRGs in the efflux of TCs, leading to poor correlations between TCs and EFP TRGs. Besides, the upstream manure samples held a similar environment to the pig gut (i.e., inadequate oxygen supply but sufficient nutrients), thus favoring the survival of anaerobic bacteria that often harbor RPP TRGs as well as contributing to the prevalence of RPP TRGs in these samples (Roberts, 2012).…”

Section: Correlation Analysismentioning

confidence: 99%

“…Polymerase chain reaction (PCR) was applied to detect the target ARGs in collected samples, including four mutant dihydropteroate synthase genes reflecting the primary mechanism of sulfonamide resistance (Sköld, 2000), and sixteen tetracycline resistance genes (TRGs) covering three main mechanisms of tetracycline resistance (ribosomal protection protein (RPP), efflux protein (EFP), and enzymatic inactivation) (Roberts, 2012;Thaker et al, 2010). Then, the frequently detected TRGs and sulfonamide resistance genes (SRGs) were further quantified by quantitative PCR (q-PCR).…”

Section: Introductionmentioning

confidence: 99%

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Dissemination of veterinary antibiotics and corresponding resistance genes from a concentrated swine feedlot along the waste treatment paths

Wang

Ben

Yang

et al. 2016

Environment International

111

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Swine feedlots are an important pollution source of antibiotics and antibiotic resistance genes (ARGs) to the environment. This study investigated the dissemination of two classes of commonly-used veterinary antibiotics, namely, tetracyclines (TCs) and sulfonamides (SAs), and their corresponding ARGs along the waste treatment paths from a concentrated swine feedlot located in Beijing, China. The highest total TC and total SA concentrations detected were 166.7 mg kg −1 and 64.5 μg kg −1 in swine manure as well as 388.7 and 7.56 μg L −1 in swine wastewater, respectively. Fourteen tetracycline resistance genes (TRGs) encoding ribosomal protection proteins (RPP), efflux proteins (EFP) and enzymatic inactivation proteins, three sulfonamide resistance genes (SRGs), and two integrase genes were detected along the waste treatment paths with detection frequencies of 33.3-75.0%. The relative abundances of target ARGs ranged from 2.74 × 10 -6 to 1.19. The antibiotics and ARGs generally declined along both waste treatment paths, but their degree of reduction was more significant along the manure treatment path. The RPP TRGs dominated in the upstream samples and then decreased continuously along both waste treatment paths, whilst the EFP TRGs and SRGs maintained relatively stable. Strong correlations between antibiotic concentrations and ARGs were observed among both manure and wastewater samples. In addition, seasonal temperature, and integrase genes, moisture content and nutrient level of tested samples could all impact the relative abundances of ARGs along the swine waste treatment paths. This study helps understand the evolution and spread of ARGs from swine feedlots to the environment as well as assess the environmental risk arising from swine waste treatment.

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Section: −2mentioning

confidence: 95%

Section: Detection Frequencies Of Argsmentioning

confidence: 99%

Section: Correlation Analysismentioning

confidence: 99%

Section: Correlation Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dissemination of veterinary antibiotics and corresponding resistance genes from a concentrated swine feedlot along the waste treatment paths

Wang

Ben

Yang

et al. 2016

Environment International

111

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Prevalence Investigation of Tetracycline Resistant Bacteria in Raw Milk

Zhang

Yang

2013

Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

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Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis

Choi

Woo

2014

Curr Microbiol

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Enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (AS) and inducible sex pheromones to exchange genetic elements in food matrix. To evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne E. faecalis were collected in Korea. Among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(M) and tet(L) genes together, followed by tet(M) (19.6 %), and tet(L) (6.8 %) alone. Also, we found the combination of tet(L)/tet(M)/tet(O) or tet(M)/tet(O). We identified two tet(S) genes including the isolate carrying tet(M) + tet(S) genes. Additionally, most E. faecalis were positive for cpd and ccf (both 96.8 %) followed by cob (57.2 %). Through mating experiments, we confirmed E. faecalis possessing the Int-Tn gene and/or any AS gene successfully transferred tet genes to JH2-2 E. faecalis, whereas neither E. faecalis carrying AS genes nor the Int-Tn gene showed the conjugation. Pulsed-field gel electrophoresis results supported a distinct pattern, implying transfer of genetic information. Our study revealed a high occurrence of tetracycline resistance genes in E. faecalis from various foods. The widespread dissemination of tetracycline resistance genes would be promoted to transfer tetracycline resistance genes by pheromone-mediated conjugation systems.

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Acquired Tetracycline Resistance Genes

Cited by 19 publications

References 89 publications

Dissemination of veterinary antibiotics and corresponding resistance genes from a concentrated swine feedlot along the waste treatment paths

Dissemination of veterinary antibiotics and corresponding resistance genes from a concentrated swine feedlot along the waste treatment paths

Prevalence Investigation of Tetracycline Resistant Bacteria in Raw Milk

Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis

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