Acquisition and reconstruction of Raman and fluorescence signals for rat leg imaging

Demers, Jennifer Lynn; Pogue, Brian W.; Leblond, Frédéric; Esmonde-White, Francis W. L.; Okagbare, Paul I.; Morris, Michael D.

doi:10.1117/12.881104

Cited by 3 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

mentioning

confidence: 99%

“…This distance relationship is vital in achieving accurate tomographic reconstruction of subsurface tissues from transcutaneous Raman data acquired from any tissue specimen. 27 The mean spectrum from all the collection fibers is shown in Fig. 2B.…”

mentioning

confidence: 99%

“…The calibration signal can potentially resolve a major challenge in spatially offset 9,30 Raman spectroscopy, where a reference signal is needed for tomographic reconstruction and also for quantification of subsurface species. 27 We have used a flat-tipped waveguide. However, reference signals could also be generated with materials terminating in a curved surface or incorporating a refractive index gradient so that they also functioned as lenses.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Okagbare

Morris

2012

Analyst

View full text Add to dashboard Cite

Advances in fiber optic probe design are moving Raman spectroscopy into the clinic, although there remain important practical problems. While much effort has been devoted to minimizing Raman and fluorescence background from fibers, less attention has been given to the need to generate reference Raman signals that can correct for variations in tissue albedo, which is important in quantifying changes in tissue composition. To address this shortcoming, we have developed a fiber optic probe that incorporates a fluorinated ethylene-propylene copolymer (FEP) cap at the end of each excitation fiber. Transmission of laser light through the transparent cap generates a 732 cm−1 Raman band whose intensity scales linearly with the laser power delivered to the tissue of interest. In our first design, the FEP cap functions as a waveguide with only a small insertion loss (~5%). Laser transmission through 1 mm of the polymer is sufficient to generate a usable reference Raman signal. We show the application of the probe to quantitative non-invasive Raman spectroscopy of animal tissues using rat leg phantoms as models. Ex-vivo Raman spectroscopy of excised rat tibia supports the use of the probe for spectroscopy of various tissues. These results provide proof of principle that the Raman probe can be used in multiple spectroscopic applications.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Okagbare

Morris

2012

Analyst

View full text Add to dashboard Cite

show abstract

“…The method yielded a contrast ratio of 145 : 1 between the cortical bone and background. The concept was further tested and optimized by Demers et al [41].…”

Section: Raman Tomographymentioning

confidence: 99%

Recent advances in the development of Raman spectroscopy for deep non‐invasive medical diagnosis

Matousek¹,

Stone²

2012

Journal of Biophotonics

149

121

View full text Add to dashboard Cite

Raman spectroscopy has recently undergone major advances in the area of deep non‐invasive characterisation of biological tissues. The progress stems from the development of spatially offset Raman spectroscopy (SORS) and renaissance of transmission Raman spectroscopy permitting the assessment of diffusely scattering samples at depths several orders of magnitude deeper than possible with conventional Raman spectroscopy. Examples of emerging applications include non‐invasive diagnosis of bone disease, cancer and monitoring of glucose levels. This article reviews this fast moving field focusing on recent developments within the medical area. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Subsurface Raman Spectroscopy in Turbid Media

Matousek

2014

Infrared and Raman Spectroscopic Imaging

View full text Add to dashboard Cite

Acquisition and reconstruction of Raman and fluorescence signals for rat leg imaging

Cited by 3 publications

References 6 publications

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Recent advances in the development of Raman spectroscopy for deep non‐invasive medical diagnosis

Subsurface Raman Spectroscopy in Turbid Media

Contact Info

Product

Resources

About