Acquisition of a B-Like Antigen by Red Blood Cells

Cameron, Caroline E.; Graham, Frances; Dunsford, I.; Sickles, Gretchen R.; Macpherson, C. R.; Cahan, Amos; Sanger, Ruth; Race, R. R.

doi:10.1136/bmj.2.5140.29

Cited by 85 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The taking up of such toxins has been put forward as an explanation of the occasional acquisition of group B antigen by red cells (Stratton and Renton, 1959), although later work demonstrating the presence of A antigen as well as B in this strain of E. colti (Gonano, Modiano and Andreis, 1961;Pettenkofer, Maassen and Bickerich, 1960) poses a further complication. It is of interest, however, that in four communications reporting the acquisition of blood group B antigen by red cells (Cameron et al, 1959;Giles et al, 1959;Marsh, Jenkins and Walther, 1959;Stratton and Renton, 1959), a total of twelve patients are described of whom eight had carcinoma of the colon or rectum.…”

Section: Discussionmentioning

confidence: 99%

Blood Group Antigens on Human Gastrointestinal Carcinoma Cells

Wk¹

1962

Br J Cancer

View full text Add to dashboard Cite

KAY (1957) reported in this journal a study, using the mixed cell agglutination technique, of bladder tumour cells. His results showed a rough correlation between the strength of agglutination and the degree of malignancy as judged histologically, and he found a general pattern of weaker agglutination associated with greater malignancy. This was not constant, however, since one well differentiated tumour was totally unagglutinable, while two anaplastic pleomorphic growths gave normal, strong agglutination. The present study, suggested by Kay's work, reports the findings, using mixed cell agglutination, on thirty-two malignant tumours of the human stomach and large bowel. METHODSSpecimens of gastric and colonic carcinomata were obtained from the operating theatre within fifteen minutes of resection. The viscus was opened, mucus and faeces gently washed away with tap water and the lesion examined. Two samples of tissue were then taken in each case. A small piece of the surface of the growth was removed with a sharp knife, and a piece of unaffected mucosa from an adjacent part of the organ was also taken. Both specimens were stored separately at -20°C. in 20 per cent glycerol in saline. The organ was then placed in formol saline for routine histological study. As soon as convenient, but usually within a few days, a mucosal cell suspension of both malignant and normal tissue was made, using a solution of oxidised ascorbic acid (Cowan, 1962). The suspensions were stored, like the tissues, in 20 per cent glycerol in saline at -200 C. A scraping of buccal cells from each patient was obtained in the post-operative period; these cells were stored in suspension in the same manner as the gastrointestinal cells and mixed cell agglutination performed on them served as a control of technique. Finally, a specimen of saliva, and often gastric juice in addition, was secured from each patient and the secretor status determined by the method of antibody-inhibition (Race and Sanger, 1958).Mixed cell agglutination was performed after the manner described by Coombs, Bedford and Rouillard (1956). Experience showed that with gastrointestinal cells, stronger mixed cell agglutination followed if the period of incubation with antibody was extended to two hours or more. With group 0 tissues, an extract of Ulex europaeus L. was used as anti H to detect H substance in the tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Blood Group Antigens on Human Gastrointestinal Carcinoma Cells

Wk¹

1962

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…It is possible to interpret the extra reactions in the positive antisera either as a small amount of anti-Bga which can only be detected by strongly reacting cells, or as cross-reactive anti-Bgb. Absorptions of se rum Ja with J. P.'s red and white cells did not remove the antibody which suggests af finity with the pseudo B antigens [4], At 80 weeks, J. P. was retested with the five anti-Bgb antisera. All the reactions…”

Section: Serial Titrationsmentioning

confidence: 99%

Increase in Strength of Red Cell Bg^a Antigen Following Infectious Mononucleosis

Morton¹,

Pickels²,

Darley³

1977

Vox Sanguinis

View full text Add to dashboard Cite

Following an attack of infectious mononucleosis, the red cells of HLA B7 patients may show a greatly increased reactivity with anti-Bg^a antibodies. This occurs from about the 3rd week of the disease, the reactivity then slowly decreasing over a period of months or years. Both specific and nonspecific reactions to other HLA antisera may also occur. Five patients were followed in detail and the reason for the increase in antigen strength was investigated without a conclusive result.

show abstract

“…Most of these cases might be ascribed to the category of acquired B-like antigens, such as those described by Cameron [6], especially since the B antigenic character of the cells was shown to be transitory in one case subjected to careful study, but this character within one particular family (the Pe family) appeared to be inherited and was stable for over two years. The variant AB phenotypes (one AjB and three A2B) perhaps involve an A2B gene, with the AXB phenotype being due to the A2B gene being partnered by an A1.…”

mentioning

confidence: 99%

Modification of Bernstein’s Multiple Allele Theory for the Inheritance of the ABO Blood Groups in the Light of Modern Genetical Concepts

Boettcher¹

1966

Vox Sanguinis

View full text Add to dashboard Cite

Acquisition of a B-Like Antigen by Red Blood Cells

Cited by 85 publications

References 0 publications

Blood Group Antigens on Human Gastrointestinal Carcinoma Cells

Blood Group Antigens on Human Gastrointestinal Carcinoma Cells

Increase in Strength of Red Cell Bg^a Antigen Following Infectious Mononucleosis

Modification of Bernstein’s Multiple Allele Theory for the Inheritance of the ABO Blood Groups in the Light of Modern Genetical Concepts

Contact Info

Product

Resources

About