Acquisition of freezing tolerance in Arabidopsis and two contrasting ecotypes of the extremophile Eutrema salsugineum (Thellungiella salsuginea)

Khanal, Nityananda; Moffatt, Barbara A.; Gray, Gordon R.

doi:10.1016/j.jplph.2015.03.011

Cited by 12 publications

(9 citation statements)

References 82 publications

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“…A distant Arabidopsis relative, Eutrema salsugineum (= Thellungiella salsuginea O.E.Schultz; Brassicaceae), has been described as exhibiting extreme cold tolerance. Its non‐acclimated LT 50 estimates range from −9 to −13°C, while acclimated LT 50 values range from −17.4 to −21.9°C, (Griffith et al., ; Khanal et al, ). Thus, the cold tolerance and acclimation capacity levels of A. lyrata and A. kamchatica are similar to those of E. salsugineum .…”

Section: Discussionmentioning

confidence: 99%

Cold tolerance in the genusArabidopsis

Armstrong

Takebayashi

Wolf

2020

American J of Botany

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Premise Cold tolerance is an important factor limiting the geographic distribution and growing season for many plant species, yet few studies have examined variation in cold tolerance extensively within and among closely related species and compared that to their geographic distribution. Methods This study examines cold tolerance within and among species in the genus Arabidopsis. We assessed cold tolerance by measuring electrolyte leakage from detached leaves in multiple populations of five Arabidopsis taxa. The temperature at which 50% of cells were lysed was considered the lethal temperature (LT50). Results We found variability within and among taxa in cold tolerance. There was no significant within‐species relationship between latitude and cold tolerance. However, the northern taxa, A. kamchatica, A. lyrata subsp. petraea, and A. lyrata subsp. lyrata, were more cold tolerant than A. thaliana and A. halleri subsp. gemmifera both before and after cold acclimation. Cold tolerance increased after cold acclimation (exposure to low, but nonfreezing temperatures) for all taxa, although the difference was not significant for A. halleri subsp. gemmifera. For all taxa except A. lyrata subsp. lyrata, the LT50 values for cold‐acclimated plants were higher than the January mean daily minimum temperature (Tmin), indicating that if plants were not insulated by snow cover, they would not likely survive winter at the northern edge of their range. Conclusions Arabidopsis lyrata and A. kamchatica were far more cold tolerant than A. thaliana. These extremely cold‐tolerant taxa are excellent candidates for studying both the molecular and ecological aspects of cold tolerance.

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Section: Discussionmentioning

confidence: 99%

Cold tolerance in the genusArabidopsis

Armstrong

Takebayashi

Wolf

2020

American J of Botany

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“…CS22504 and Yukon accession, stock no. CS22664) were obtained from the Arabidopsis Biological Resource Center (ABRC, The Ohio State University, Columbus, OH, USA) were germinated and maintained in controlled environment growth chambers (Model PGR15; Conviron, Winnipeg, MB, Canada) using the non-acclimating growth conditions described in Table 1 [ 39 ]. These are referred to as Yukon, Shandong and Arabidopsis growth regimes.…”

Section: Methodsmentioning

confidence: 99%

“…Eutrema has been used as a model for the study of cold acclimation and freezing tolerance [ 31 , 38 , 39 ]. While the study of [ 39 ] demonstrated that Eutrema and Arabidopsis did not differ in their constitutive level of freezing tolerance or short-term cold acclimation capacity, Eutrema outperformed Arabidopsis in long-term acclimation capacity suggesting a wider phenotypic plasticity for the trait of freezing tolerance. Furthermore, it was demonstrated that growth conditions, specifically irradiance, were determinants of the level of freezing tolerance attained during cold acclimation suggesting a role for photosynthetic processes in adaptive stress responses.…”

Section: Introductionmentioning

confidence: 99%

Differential Mechanisms of Photosynthetic Acclimation to Light and Low Temperature in Arabidopsis and the Extremophile Eutrema salsugineum

Khanal

Bray

Grisnich

et al. 2017

Plants

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Photosynthetic organisms are able to sense energy imbalances brought about by the overexcitation of photosystem II (PSII) through the redox state of the photosynthetic electron transport chain, estimated as the chlorophyll fluorescence parameter 1-qL, also known as PSII excitation pressure. Plants employ a wide array of photoprotective processes that modulate photosynthesis to correct these energy imbalances. Low temperature and light are well established in their ability to modulate PSII excitation pressure. The acquisition of freezing tolerance requires growth and development a low temperature (cold acclimation) which predisposes the plant to photoinhibition. Thus, photosynthetic acclimation is essential for proper energy balancing during the cold acclimation process. Eutrema salsugineum (Thellungiella salsuginea) is an extremophile, a close relative of Arabidopsis thaliana, but possessing much higher constitutive levels of tolerance to abiotic stress. This comparative study aimed to characterize the photosynthetic properties of Arabidopsis (Columbia accession) and two accessions of Eutrema (Yukon and Shandong) isolated from contrasting geographical locations at cold acclimating and non-acclimating conditions. In addition, three different growth regimes were utilized that varied in temperature, photoperiod and irradiance which resulted in different levels of PSII excitation pressure. This study has shown that these accessions interact differentially to instantaneous (measuring) and long-term (acclimation) changes in PSII excitation pressure with regard to their photosynthetic behaviour. Eutrema accessions contained a higher amount of photosynthetic pigments, showed higher oxidation of P700 and possessed more resilient photoprotective mechanisms than that of Arabidopsis, perhaps through the prevention of PSI acceptor-limitation. Upon comparison of the two Eutrema accessions, Shandong demonstrated the greatest PSII operating efficiency (ΦPSII) and P700 oxidizing capacity, while Yukon showed greater growth plasticity to irradiance. Both of these Eutrema accessions are able to photosynthetically acclimate but do so by different mechanisms. The Shandong accessions demonstrate a stable response, favouring energy partitioning to photochemistry while the Yukon accession shows a more rapid response with partitioning to other (non-photochemical) strategies.

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“…In A. 316 thaliana, the change in chlorophyll fluorescence from before to after cold shock 317 correlates with tolerance to sub-zero temperatures measured by electrolyte leakage or 318 survival and, therefore, this is regarded as an indicator of cold tolerance (Ehlert & 319 Hincha 2008; Heo et al 2014;Khanal et al 2015). Therefore, our result also 320 represents evidence for among-family (thus possibly among-population) variance in 321 cold tolerance.…”

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confidence: 75%

Tissue culture as a source of replicates in non-model plants: variation in cold tolerance inArabidopsis lyratassp.petraea

Kenta

Edwards

Butlin

et al. 2016

Preprint

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Whilst genotype–environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates—a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and “somaclonal” variation. Here we examined the effects of tissue culturing by clonal growth on the response to cold treatment in the photosynthetic efficiency (chlorophyll fluorescence measurements for Fv/Fm, Fv′/Fm′ and ФPSII, representing maximum efficiency of photosynthesis for dark- and light-adapted leaves, and the actual electron transport operating efficiency, respectively), compared to half-sibling seedlings from three different families ofArabidopsis lyratassp.petraea. Somaclonal variation was limited and we could successfully detect within-family variation in change in chlorophyll fluorescence by cold shock with the help of tissue-culture derived replicates. Icelandic and Norwegian families exhibited higher chlorophyll fluorescence, suggesting higher cold tolerance, than a Swedish family. Although the main effect of tissue culture on Fv/Fm, Fv′/Fm′ and ФPSII was small, there were significant interactions between tissue culture and family, suggesting that the effect of tissue culture is genotype-specific. Tissue-cultured plantlets were less affected by cold treatment than seedlings, but to a different extent in each family. These interactive effects, however, were comparable to, or much smaller than the single effect of family. These results suggest that tissue culture is a useful method for obtaining genetically homogenous replicates for studying genotype‐environment interaction related to adaptively relevant phenotypes, such as cold tolerance, in non-model outcrossing plants.

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Acquisition of freezing tolerance in Arabidopsis and two contrasting ecotypes of the extremophile Eutrema salsugineum (Thellungiella salsuginea)

Cited by 12 publications

References 82 publications

Cold tolerance in the genusArabidopsis

Cold tolerance in the genusArabidopsis

Differential Mechanisms of Photosynthetic Acclimation to Light and Low Temperature in Arabidopsis and the Extremophile Eutrema salsugineum

Tissue culture as a source of replicates in non-model plants: variation in cold tolerance inArabidopsis lyratassp.petraea

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