Acquisition of Meiotic Competence Is Related to the Functionality of the Phosphoinositide/Calcium Signaling Pathway in the Mouse Oocyte

Lefèvre, Brigitte; Nagyová, Eva; Pesty, Arlette; Testart, J

doi:10.1006/excr.1997.3720

Cited by 22 publications

(19 citation statements)

References 18 publications

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“…By day 9, isolated spikes were identified in a few oocytes and a more pronounced signalling was detected on day 12, with a series of fairly regular spontaneous calcium oscillations. These results are consistent with previous work showing that immature oocytes recovered from preantral follicles with 2-3 cell layers retrieved from prepubertal mice display no intracellular calcium signalling (Lefevre et al, 1997;Gomes et al, 1999). They also demonstrate the progressive acquisition of calcium signalling during in vitro oogenesis, as observed in vivo (Lefevre et al, 1997).…”

Section: Discussionsupporting

confidence: 83%

“…These results are consistent with previous work showing that immature oocytes recovered from preantral follicles with 2-3 cell layers retrieved from prepubertal mice display no intracellular calcium signalling (Lefevre et al, 1997;Gomes et al, 1999). They also demonstrate the progressive acquisition of calcium signalling during in vitro oogenesis, as observed in vivo (Lefevre et al, 1997). The appearance of intracellular calcium oscillations is one of the first steps in meiosis resumption in mature oocytes, occurring just before GVBD (Carroll et al, 1994;Homa, 1995;Lefevre et al, 1995); they depend clearly on the phosphoinositide pathway (Coticchio and Fleming, 1998;Pesty et al, 1998).…”

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

“…Oocytes isolated from preantral follicles are unable to undergo germinal vesicle breakdown (GVBD) in vitro, but the rate of GVBD steadily increases with oocyte diameter (Eppig et al, 1994;Lefevre et al, 1997). During oogenesis, the acquisition of meiotic competence is associated with a gradual increase in ability to perform spontaneous Ca 2+ oscillations not seen in incompetent oocytes (Lefevre et al, 1997). In fully grown oocytes, meiosis resumption has been shown to be related to spontaneous cytoplasmic InsP 3 -dependent calcium oscillations (Carroll et al, 1994;Lefevre et al, 1995;Coticchio and Fleming, 1998;Pesty et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Multiparameter assessment of mouse oogenesis during follicular growth in vitro

Pesty¹,

Miyara²,

Debey³

et al. 2006

MHR: Basic Science of Reproductive Medicine

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Comparison of oocyte development within the follicle in vitro and in vivo has a major impact on research into ovarian physiology and clinical practice. Despite major differences in ovarian physiology between rodents and humans, mice provide a useful model for studies of the endocrine and paracrine mechanisms controlling follicular development. In this study, early preantral follicles were isolated from 12-day-old mice and cultured individually in microdrops under oil during 6, 9 or 12 days. Taking into account previous observations, several oocyte criteria (diameter, chromatin configuration, transcriptional activity, intracytoplasmic calcium signalling and ability to undergo meiosis) were assessed to check that the development pattern of oocytes during follicle growth in vitro was similar to that already observed for oocytes developing in vivo, and that they reached the fertilizable oocyte stage. Results indicate that, during the 12-day-culture period, the oocytes grew until 74.3 ± 4.2 mm, they became transcriptionally quiescent with a surrounded nucleolus (SN) chromatin organization, 50% of them exhibited regular calcium signals and 73.4% of them resumed meiosis. These data demonstrate that the protocol used generates oocytes with characteristics similar to oocytes allowed to mature fully in vivo and that it could be useful to set up the experimental culture of human ovarian follicles.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiparameter assessment of mouse oogenesis during follicular growth in vitro

Pesty¹,

Miyara²,

Debey³

et al. 2006

MHR: Basic Science of Reproductive Medicine

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show abstract

“…We concluded that a specific nuclear PI cycle is present in the mouse oocyte and meiosis resumption requires a specific nuclear phosphoinositide-dependent Ca [14]. Moreover, we observed that the changes in the distribution of the chromatin in the germinal vesicle (GV) occurring before germinal vesicle breakdown (GVB) were related to the occurrence of these spontaneous oscillations [13], i.e.…”

mentioning

confidence: 81%

The germinal vesicle of the mouse oocyte contains elements of the phosphoinositide cycle: what is their role at meiosis resumption?

Avazeri¹,

Pesty²,

Lefèvre³

1998

Reprod. Nutr. Dev.

Self Cite

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-The role of the nuclear phosphoinositide (PI) cycle during meiotic resumption in mouse oocytes was examined. First, using indirect immunofluorescence staining with specific monoclonal antibodies (mAbs) against elements of this cycle, the presence of inositol trisphosphate receptors (IP 3 Rs) (IP 3 R-1 or IP 3 R-3) or phosphoinositide-phospholipase (PLC) isoforms (PLCfII or PLCyl) was monitored in the germinal vesicle (GV). Using confocal laser scanning microscopy, we analysed the effects of the nuclear microinjection of these antibodies on both spontaneous nuclear calcium oscillations and meiosis resumption. Immunostainings showed that IP 3 R-1 and PLC O isoforms were both present in the GV, whereas IP 3 R-3 and PLCyl isoforms were not. The anti-IP 3 R-1 mAbs or the anti-PLC(31 mAbs microinjected into the GV, induced inhibition of both the nuclear Ca 2+ oscillations and the meiotic process, whereas the anti-IP 3 R-3 mAbs and the anti-PLC!yl mAbs did not. We concluded that a specific nuclear PI cycle is present in the mouse oocyte and meiosis resumption requires a specific nuclear phosphoinositide-dependent Ca

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The thiol reagent, thimerosal, irreversibly inhibits meiosis reinitiation in mouse oocyte when applied during a very early and narrow temporal window: A pharmacological analysis

Alexandre

Delsinne

Goval

2003

Molecular Reproduction Devel

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The effect of the sulfhydryl reagent, thimerosal (TMS) on meiosis resumption in germinal vesicle (GV)-stage denuded mouse oocytes was studied. It irreversibly inhibits both GV breakdown (GVBD) and the first polar body (pb1) extrusion in concentration- and time-dependent manners, the most striking result being the very early and narrow temporal window during which denuded primary oocytes released from their follicle are susceptible to a pulse of the drug. This inhibition is bypassed by dithiothreitol (DTT) with an efficiency declining with time, while thiosalicylic acid (TA), an analog of TMS devoid of the mercury atom, has no effect on meiosis reinitiation. These results strongly suggest that the inhibitory effect of TMS is a consequence of its sulfhydryl group oxidising activity. The molecular target(s) of this inhibitory oxidation should however be identified. In contrast to DTT, okadaic acid (OA), known to bypass the inhibitory effect of drugs interfering with protein kinase activities, only induces chromatin condensation and GVBD in TMS-pulsed oocytes with a delay of about 8 hr as compared to the control situation. This confirms that a very early thiol oxidation induced by TMS exerts a much more dramatic effect on resumption on meiosis than any pharmacological manipulation of protein kinase activities leading to activation of MPF.

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Acquisition of Meiotic Competence Is Related to the Functionality of the Phosphoinositide/Calcium Signaling Pathway in the Mouse Oocyte

Cited by 22 publications

References 18 publications

Multiparameter assessment of mouse oogenesis during follicular growth in vitro

Multiparameter assessment of mouse oogenesis during follicular growth in vitro

The germinal vesicle of the mouse oocyte contains elements of the phosphoinositide cycle: what is their role at meiosis resumption?

The thiol reagent, thimerosal, irreversibly inhibits meiosis reinitiation in mouse oocyte when applied during a very early and narrow temporal window: A pharmacological analysis

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