Acquisition of pulsed electric fields resistance in Staphylococcus aureus after exposure to heat and alkaline shocks

Cebrián, Guillermo; Raso, Javier; Condón, S.; Mañas, Pilar

doi:10.1016/j.foodcont.2011.10.048

Cited by 31 publications

(9 citation statements)

References 51 publications

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“…The opportunity to thermally inactivate S. aureus depends upon its temperature sensitivity and its ability for thermo-tolerance 27-29 . It has been shown that heating S. aureus in tryptic soy broth (TSB) solution at 60°C for 20 min resulted in a ∼3 log reduction, while heating S. aureus at 70°C for 2 min resulted in a ∼5 log reduction 29 .…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticle Targeted Hyperthermia of Cutaneous Staphylococcus aureus Infection

et al. 2012

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The incidence of wound infections that do not adequately respond to standard-of-care antimicrobial treatment has been increasing. To address this challenge, a novel antimicrobial magnetic thermotherapy platform has been developed in which a high-amplitude, high-frequency, alternating magnetic field (AMF) is used to rapidly heat magnetic nanoparticles that are bound to Staphylococcus aureus (S. aureus). The antimicrobial efficacy of this platform was evaluated in the treatment of both an in vitro culture model of S. aureus biofilm and a mouse model of cutaneous S. aureus infection. We demonstrated that an antibody-targeted magnetic nanoparticle bound to S. aureus was effective at thermally inactivating S. aureus and achieving accelerated wound healing without causing tissue injury.

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Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticle Targeted Hyperthermia of Cutaneous Staphylococcus aureus Infection

et al. 2012

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“…Somolinos et al (2008) found that none of the 4 E. coli strains tested in their study increased their PEF resistance as a consequence of a heat or a cold shock. In addition, although cross-resistance induced by previous heat treatments has been widely demonstrated (Arroyo et al, 2012;Cebrián et al, 2012;Pagán and Mackey, 2000), influence of a previous PEF treatment on following lethal stresses applied for food preservation, such as heat or antimicrobial compounds, has not been reported in the literature. Only Álvarez and Heinz (2007) reported an increase of ultrasound resistance in previously PEF-treated cells, which was attributable to a membrane reorganization caused by PEF treatment.…”

Section: Cross-resistance Evaluation After a Pef Treatmentmentioning

confidence: 99%

Transcriptomic analysis of Escherichia coli MG1655 cells exposed to pulsed electric fields

Chueca

Pagán

García-Gonzalo

2015

Innovative Food Science & Emerging Technologies

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“…sakazakii , enhanced PEF tolerance has been also observed after a cold (Evrendilek and Zhang 2003; Somolinos et al. 2008) and alkaline shocks (Cebrián et al. 2011) with other species.…”

Section: Discussionmentioning

confidence: 88%

“…Similarly, higher PEF tolerance was also observed for E. coli after exposure to an acid shock at pH 3·6 – but not at pH 5·2 (Evrendilek and Zhang 2003) – and with Staph. aureus pre‐exposed to heat (Cebrián et al. 2011).…”

Section: Discussionmentioning

confidence: 99%

Development of resistance in Cronobacter sakazakii ATCC 29544 to thermal and nonthermal processes after exposure to stressing environmental conditions

Arroyo¹,

Cebrián²,

Condón³

et al. 2012

Journal of Applied Microbiology

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Aims: The objective was to study the response of Cronobacter sakazakii ATCC 29544 cells to heat, pulsed electric fields (PEF), ultrasound under pressure (Manosonication, MS) and ultraviolet light (UV‐C) treatments after exposure to different sublethal stresses that may be encountered in food‐processing environments. Methods and Results: Cronobacter sakazakii stationary growth‐phase cells (30°C, 24 h) were exposed to acid (pH 4·5, 1 h), alkaline (pH 9·0, 1 h), osmotic (5% NaCl, 1 h), oxidative (0·5 mmol l−1 H2O2, 1 h), heat (47·5°C, 1 h) and cold (4°C, 4 h) stress conditions and subjected to the subsequent challenges: heat (60°C), PEF (25 kV cm−1, 35°C), MS (117 μm, 200 kPa, 35°C) and UV‐C light (88·55 mW cm−2, 25°C) treatments. The inactivation kinetics of C. sakazakii by the different technologies did not change after exposure to any of the stresses. The combinations of sublethal stress and lethal treatment that were protective were: heat shock–heat, heat shock–PEF and acid pH–PEF. Conversely, the alkaline shock sensitized the cells to heat and UV‐C treatments, the osmotic shock to heat treatments and the oxidative shock to UV‐C treatments. The maximum adaptive response was observed when heat‐shocked cells were subjected to a heat treatment, increasing the time to inactivate 99·9% of the population by 1·6 times. Conclusions: Cronobacter sakazakii resistance to thermal and nonthermal preservation technologies can increase or decrease as a consequence of previous exposure to stressing conditions. Significance and Impact of the Study: The results help in understanding the physiology of the resistance of this emerging pathogen to traditional and novel preservation technologies.

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Acquisition of pulsed electric fields resistance in Staphylococcus aureus after exposure to heat and alkaline shocks

Cited by 31 publications

References 51 publications

Magnetic Nanoparticle Targeted Hyperthermia of Cutaneous Staphylococcus aureus Infection

Magnetic Nanoparticle Targeted Hyperthermia of Cutaneous Staphylococcus aureus Infection

Transcriptomic analysis of Escherichia coli MG1655 cells exposed to pulsed electric fields

Development of resistance in Cronobacter sakazakii ATCC 29544 to thermal and nonthermal processes after exposure to stressing environmental conditions

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