Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

Ding, Yichen; Teo, Jeanette; Drautz–Moses, Daniela I.; Schuster, Stephan C.; Givskov, Michael; Yang, Liang

doi:10.1038/s42003-018-0064-0

Cited by 36 publications

(35 citation statements)

References 69 publications

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“…MPF T class ICEs were targeted by more complex elements, such as the composite transposons carrying bla SPM-1 and bla NDM-1 ( Table 1 ). The bla NDM-1 gene was identified in Singapore in ICE Tn 4371 6385 and associated with ST308, as recently reported [63]. The bla NDM-1 was flanked by two IS CR24 -like transposases.…”

Section: Resultssupporting

confidence: 53%

Carbapenemases on the move: it’s good to be on ICE

Botelho

Roberts

León‐Sampedro

et al. 2018

Preprint

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22 37 38 39 40 41 42 43 44 45 Among the non-fermenting Gram-negative bacteria, the Pseudomonas genus is the one with 46 the highest number of species [1, 2]. Pseudomonas aeruginosa, an opportunistic human 47 pathogen associated with an ever-widening array of life-threatening acute and chronic 48 infections, is the most clinically relevant species within this genus [3-5]. P. aeruginosa is one 49 of the CDC "ESKAPE" pathogens -Enterococcus faecium, Staphylococcus aureus, Klebsiella 50 pneumoniae, Acinetobacter baumannii, P. aeruginosa and Enterobacter species -, 51 emphasizing its impact on hospital infections and the ability of this microorganism to "escape" 52 the activity of antibacterial drugs [6]. P. aeruginosa can develop resistance to a wide range of 53 antibiotics due to a combination of intrinsic, adaptive, and acquired resistance mechanisms, 54 such as the reduction of its outer membrane permeability, over-expression of constitutive or 55 inducible efflux pumps, overproduction of AmpC cephalosporinase, and the acquisition of 56 antibiotic resistance genes (ARGs) through horizontal gene transfer (HGT) [4, 7, 8]. P. 57aeruginosa has a non-clonal population structure, punctuated by specific sequence types 58 (STs) that are globally disseminated and frequently linked to the dissemination of ARGs [4, 9]. 59 These STs have been designated as high-risk clones, of which major examples are ST111, 60 ST175, ST235 and ST244. 61

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Section: Resultssupporting

confidence: 53%

Carbapenemases on the move: it’s good to be on ICE

Botelho

Roberts

León‐Sampedro

et al. 2018

Preprint

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“…ICE and plasmids both use the same type IV secretion system for conjugative transfer (31,(33)(34)(35), and the difference between ICE and plasmids comes from their ability to integrate into the chromosome. Like plasmids, ICE contain cargo genes (29,(36)(37)(38), and P. aeruginosa ICE have been implicated in a range of traits including xenobiotic compound degeneration (39), antibiotic resistance (32,(40)(41)(42), and virulence formation (43). Although plasmids and ICE share many similarities, ICE are abundant in P. aeruginosa, whereas plasmids are thought to be comparatively rare.…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas systems restrict horizontal gene transfer inPseudomonas aeruginosa

Wheatley

MacLean

2020

Preprint

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CRISPR-Cas systems provide bacteria and archaea with an adaptive immune system that targets foreign DNA. However, the xenogenic nature of immunity provided by CRISPR-Cas raises the possibility that these systems may constrain horizontal gene transfer. Here we test this hypothesis in the opportunistic pathogen Pseudomonas aeruginosa, which has emerged an important model system for understanding CRISPR-Cas function. Across the diversity of P. aeruginosa, active CRISPR-Cas systems are associated with smaller genomes and a reduced GC content, suggesting that CRISPR-Cas inhibits the acquisition of foreign DNA. Although phage are the major target of CRISPR-Cas spacers, more than 80% of isolates with an active CRISPR-Cas system have spacers that target integrative conjugative elements (ICE) or the conserved conjugative transfer machinery used by plasmids and ICE. Consistent with these results, genomes containing active CRISPR-Cas systems harbor a lower abundance of both prophage and ICE. Crucially, spacers in genomes with active CRISPR-Cas systems map to ICE and phage that are integrated into the chromosomes of closely related genomes lacking CRISPR-Cas immunity, providing direct evidence that CRISPR-Cas constrains horizontal gene transfer in these lineages. In conclusion, we find that CRISPR-Cas acts as an important constraint to horizontal gene transfer, suggesting that CRISPR-Cas may constrain the ability of this pathogen to adapt to new niches and stressors.

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“…In the present study, HRM and different primers were used to identify P. aeruginosa PASGNDM699 strain. In a study,by Ding et al [25] Proposed the resistance of PASGNDM699 strain to a wide range of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of High-Resolution Melting Curve Analysis (HRM) assay for Detection of Pseudomonas aeruginosa PASGNDM699: A dangerous New Delhi metallo-β-lactamase (NDM) strain

Dehbashi¹,

Tahmasebi²,

Arabestani³

2019

Preprint

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Background: New Delhi metallo-β-lactamase (NDM-1) is a broad spectrum β-lactamase that is able to inactivate all β-lactams except aztreonam, as is typical of metallo-β-lactamases. NDM-1 producers in Pseudomonas aeruginosa, especially PASGNDM699 strain, cause a range of infections such as urinary tract, diarrhoea and soft tissue infections. The aim of this study was to Standardization of High-Resolution Melting Curve Analysis (HRM) assay for detection of P. aeruginosa, especially PASGNDM699 strain. Methods: The HRM method was done on standard strains of P. aeruginosa strains. 9-fold Serial dilutions of known DNA concentrations, extracted from standard isolates were prepared and tested by Real Time Melting curve and HRM assay. Data analysis was performed using the StepOne Software v2.3 and HRM Software v3.0.1 (Applied Biosystems, Ltd). Results: Based on the results of the Real Time PCR assay and melt curve analysis, melting point temperatures of the N-1, N-2 and N-3 amplicon for isolates identified as NDM strains were 87.57°C, 76.92°C and 82.97°C, respectively. Furthermore, melting point temperatures of the blaVIM, blaSPM and blaSIM amplicon for isolates identified as MBL strains were 84.56°C, 85.35°C and 86.62°C, respectively. Due to the analytical specificity of the primers, all dilutions with a similar Tm and melt peaks were obtained in the melting curves. Moreover, the analytical sensitivity of NDM primer were able to detected 100CFU/mL, 103CFU/mL and 104CFU/mL of standard DAN by N-1, N-2 and N-3 primers, respectively. Also, according to analytical sensitivity of MBL primers, blaVIM was able detected of 100CFU/mL, blaSPM primer 105CFU/mL and blaSIM primer 102CFU/mL of PASGNDM699 strain. HRM results showed that N-1 primers with 55 bp and blaVIM primers with 124 bp had the highest sensitivity and specificity for P. aeruginosa PASGNDM699 strain identification. Conclusion: The data from our study indicated that the sensitivity and specificity of the HRM method linked to the primer length and the fluorescent dye. Further, we can identify antibiotic resistance in substrates such as P. aeruginosa PASGNDM699 by software analysis and melting curve analysis.

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Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

Cited by 36 publications

References 69 publications

Carbapenemases on the move: it’s good to be on ICE

Carbapenemases on the move: it’s good to be on ICE

CRISPR-Cas systems restrict horizontal gene transfer inPseudomonas aeruginosa

Evaluation of High-Resolution Melting Curve Analysis (HRM) assay for Detection of Pseudomonas aeruginosa PASGNDM699: A dangerous New Delhi metallo-β-lactamase (NDM) strain

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