Acquisition of the ability for Rhodopseudomonas palustris to degrade chlorinated benzoic acids as the sole carbon source

Oda, Yuji; Vries, Ynte P. de; Forney, Larry J.; Gottschal, Jan C.

doi:10.1111/j.1574-6941.2001.tb00891.x

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…In this work, we isolated a strain of R. palustris that acquired the ability to use syringic acid as a growth substrate under photoheterotrophic conditions. Our strategy of incrementally exposing cultures to higher concentrations of syringic acid, while at the same time reducing the availability of the known growth substrates benzoic acid and 4-HBA, has been shown to be conducive to adaptation and the acquisition of new metabolic activities in R. palustris (38,39) and other bacteria (40). Our analysis of this adapted strain, SA008.1.07, has provided important new knowledge on the bacterial metabolism of syringic acid.…”

Section: Figmentioning

confidence: 97%

Anaerobic Degradation of Syringic Acid by an Adapted Strain of Rhodopseudomonas palustris

Oshlag

Morse

et al. 2020

Appl Environ Microbiol

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While lignin represents a major fraction of the carbon in plant biomass, biological strategies to convert the components of this heterogeneous polymer into products of industrial and biotechnological value are lacking. Syringic acid (3,5-dimethoxy-4-hydroxybenzoic acid) is a by-product of lignin degradation, appearing in lignocellulosic hydrolysates, deconstructed lignin streams, and other agricultural products. Rhodopseudomonas palustris CGA009 is a known degrader of phenolic compounds under photoheterotrophic conditions via the benzoyl coenzyme A (CoA) degradation (BAD) pathway. However, R. palustris CGA009 is reported to be unable to metabolize meta-methoxylated phenolics, such as syringic acid. We isolated a strain of R. palustris (strain SA008.1.07), adapted from CGA009, which can grow on syringic acid under photoheterotrophic conditions, utilizing it as a sole source of organic carbon and reducing power. An SA008.1.07 mutant with an inactive benzoyl-CoA reductase structural gene was able to grow on syringic acid, demonstrating that the metabolism of this aromatic compound is not through the BAD pathway. Comparative gene expression analyses of SA008.1.07 implicated the involvement of products of the vanARB operon (rpa3619, rpa3620, rpa3621), which has been described as catalyzing aerobic aromatic ring demethylation in other bacteria, in anaerobic syringic acid degradation. In addition, experiments with a vanARB deletion mutant demonstrated the involvement of the vanARB operon in anaerobic syringic acid degradation. These observations provide new insights into the anaerobic degradation of meta-methoxylated and other aromatics by R. palustris. IMPORTANCE Lignin is the most abundant aromatic polymer on Earth and a resource that could eventually substitute for fossil fuels as a source of aromatic compounds for industrial and biotechnological applications. Engineering microorganisms for the production of aromatic-based biochemicals requires detailed knowledge of the metabolic pathways for the degradation of aromatics that are present in lignin. Our isolation and analysis of a Rhodopseudomonas palustris strain capable of syringic acid degradation reveal a previously unknown metabolic route for aromatic degradation in R. palustris. This study highlights several key features of this pathway and sets the stage for a more complete understanding of the microbial metabolic repertoire required to metabolize aromatic compounds from lignin and other renewable sources.

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Section: Figmentioning

confidence: 97%

Anaerobic Degradation of Syringic Acid by an Adapted Strain of Rhodopseudomonas palustris

Oshlag

Morse

et al. 2020

Appl Environ Microbiol

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show abstract

“…In this work, we isolated a strain of R. palustris that acquired the ability to use syringic acid as a growth substrate under photoheterotrophic conditions. Our strategy of incrementally exposing cultures to higher concentrations of syringic acid, while at the same time reducing the availability of the known growth substrates benzoic acid and 4-HBA, has been shown to be conducive to adaptation and acquisition of new metabolic activities in R. palustris (52, 53) and other bacteria (54). Our analysis of this adapted strain, SA008.1.07 has provided important new knowledge on the bacterial metabolism of syringic acid.…”

Section: Resultsmentioning

confidence: 99%

Anaerobic degradation of syringic acid by an adapted strain ofRhodopseudomonas palustris

Oshlag

Morse

et al. 2019

Preprint

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18While lignin represents a major fraction of the carbon in plant biomass, biological strategies to 19 convert the components of this heterogenous polymer into products of industrial and 20 biotechnological value are lacking. Syringic acid (3,5-dimethoxy-4-hydroxybenzoic acid) is a 21 byproduct of lignin degradation, appearing in lignocellulosic hydrolysates, deconstructed lignin 22 streams, and other agricultural products. Rhodopseudomonas palustris CGA009 is a known 23 degrader of phenolic compounds under photoheterotrophic conditions, via the benzoyl-CoA 24 degradation (BAD) pathway. However, R. palustris CGA009 is reported to be unable to 25 metabolize meta-methoxylated phenolics such as syringic acid. We isolated a strain of R. palustris 26 (strain SA008.1.07), adapted from CGA009, which can grow on syringic acid under 27 photoheterotrophic conditions, utilizing it as a sole source of organic carbon and reducing power. 28 An SA008.1.07 mutant with an inactive benzoyl-CoA reductase structural gene was able to grow 29 on syringic acid, demonstrating that the metabolism of this aromatic compound is not through the 30 BAD pathway. Comparative gene expression analyses of SA008.1.07 implicated the involvement 31 of products of the vanARB operon (rpa3619-rpa3621), which has been described as catalyzing 32 aerobic aromatic ring demethylation in other bacteria, in anaerobic syringic acid degradation. In 33 addition, experiments with a vanARB deletion mutant demonstrated the involvement of the 34 vanARB operon in anaerobic syringic acid degradation. These observations provide new insights 35 into the anaerobic degradation of meta-methoxylated and other aromatics by R. palustris. 36 IMPORTANCE 37Lignin is the most abundant aromatic polymer on Earth and a resource that could eventually 38 substitute for fossil fuels as a source of aromatic compounds for industrial and biotechnological 39 applications. Engineering microorganisms for production of aromatic-based biochemicals requires 40 3 detailed knowledge of metabolic pathways for the degradation of aromatics that are present in 41 lignin. Our isolation and analysis of a Rhodopseudomonas palustris strain capable of syringic acid 42 degradation reveals a previously unknown metabolic route for aromatic degradation in R. palustris. 43 This study highlights several key features of this pathway and sets the stage for a more complete 44 understanding of the microbial metabolic repertoire to metabolize aromatic compounds from lignin 45 and other renewable sources. 46 48 renewable source of carbon for the bio-based production of compounds that are currently derived 49 from petroleum. Unfortunately, the ability to derive chemicals of commercial, chemical, or 50 medicinal value from lignin is limited by information needed to improve the biological conversion 51 of the aromatics in lignin into valuable products. We are interested in improving our understanding 52 of how bacteria metabolize the aromatic building blocks in lignin and using this information to 53 devel...

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“…In several studies the anoxic phototrophic bacterium, Rhodopseudomonas palustris, was shown to degrade 3-CBc completely to CO 2 and cell biomass in the presence of light and absence of oxygen as long as benzoic acid was present (Kamal and Wyndham 1990;Oda et al 2001Oda et al , 2004. Rh.…”

Section: Aerobic Bacterial Growth On Chlorinated Benzoates As a Sole mentioning

confidence: 99%

“…Rh. palustris could be acclimatized to degrade 3-CBc as the sole source of carbon (Oda et al 2001(Oda et al , 2004. The pathway of 3-CBc degradation in Rh.…”

Section: Aerobic Bacterial Growth On Chlorinated Benzoates As a Sole mentioning

confidence: 99%

Microbial transformation of chlorinated benzoates

Field

Sierra-Álvarez

2008

Rev Environ Sci Biotechnol

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Acquisition of the ability for Rhodopseudomonas palustris to degrade chlorinated benzoic acids as the sole carbon source

Cited by 11 publications

References 27 publications

Anaerobic Degradation of Syringic Acid by an Adapted Strain of Rhodopseudomonas palustris

Anaerobic Degradation of Syringic Acid by an Adapted Strain of Rhodopseudomonas palustris

Anaerobic degradation of syringic acid by an adapted strain ofRhodopseudomonas palustris

Microbial transformation of chlorinated benzoates

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