Acquisition of the potential for sperm motility in steelhead (Oncorhynchus mykiss): effect of pH on dynein ATPase

Woolsey, J.; Ingermann, Rolf L.

doi:10.1023/b:fish.0000035896.84954.f6

Cited by 25 publications

(22 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Water pH has a direct influence on these processes. Gametes and embryos of most fish species are sensitive to exposure to acid waters; the degree of sensitivity depends on the ontogenetic stage (DEPÊCHE; BILLARD, 1994;WOOLSEY;INGERMANN, 2004). Generally, embryos and larvae are more sensitive than juveniles and adults (DEPÊCHE; BILLARD, 1994;ZANIBONI-FILHO et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…When P. lineatus is kept in captivity, its reproductive cycle is interrupted (MORAES et al, 2004); hence, hormonal manipulation is required to induce the final gonadal maturation and the release of gametes for further artificial fertilization (WOYNAROVICH;HORVÁTH, 1983). During artificial reproduction, the gametes and embryos exposed to water are susceptible to abiotic features (JONES; REYNOLDS, 1997;NASH, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of water pH on gamete activation, embryonic development, and larval normality in Prochilodus lineatus

et al. 2015

View full text Add to dashboard Cite

We investigated the effects of breeding water pH on the spermatic motility, artificial fertilization, and initial development of offspring in curimba, Prochilodus lineatus. After hormonal induction, we conducted gamete activation, artificial fertilization, and embryo incubation in water with pH values of 4.43 ± 0.13, 5.82 ± 0.14, 7.37 ± 0.10, 8.21 ± 0.06, and 9.57 ± 0.16. When the water pH was 6.65, spermatic motility was maintained for ≤25.21 s (P < 0.05). The highest fertilization rates (P < 0.05) were obtained when the water pH ranged from 5.82 ± 0.14 to 8.21 ± 0.06, and the highest hatching rates (P < 0.05) were observed when the water pH was 7.37 ± 0.10. A water pH of between 7.37 ± 0.10 and 8.21 ± 0.06 resulted in more complete formation of the perivitelline space (P < 0.05); additionally, embryos incubated in alkaline waters produced a higher percentage of normal larvae (P < 0.05), despite increased mortality levels. Our results indicate that the pH of the water used for gamete activation, artificial oocyte fertilization, and incubation of eggs and larvae of P. lineatus should be ~7, in order to promote successful breeding and normal larval production. Key words: Artificial reproduction, curimba, fertilization, fish, sperm motility, water pH ResumoForam investigados os efeitos do pH da água de criação na motilidade espermática, fertilização artificial, e no desenvolvimento inicial da prole de curimba, Prochilodus lineatus. Após a indução hormonal, realizamos a ativação dos gametas, fecundação artificial e incubação dos embriões em água com os valores de pH de 4,43 ± 0,13, 5,82 ± 0,14, 7,37 ± 0,10, 8,21 ± 0,06 e 9,57 ± 0,16. Quando o pH da água foi de 6,65, a motilidade espermática foi mantida em ≤25.21 s (P <0,05). As maiores taxas de fertilização (P <0,05) foram obtidas quando o pH da água variou de 5,82 ± 0,14 a 8,21 ± 0,06, e as maiores taxas de eclosão (P <0,05) foram observadas quando o pH da água foi de 7,37 ± 0,10. O pH da água entre 7,37 ± 0,10 e 8,21 ± 0,06 resultou na formação mais completa do espaço perivitelino (P <0,05); Além disso, os embriões incubados em águas alcalinas tiveram maior porcentagem de larvas normal (P <0,05), apesar do aumento dos níveis de mortalidade. Os nossos resultados indicam que o pH da água utilizada para a ativação dos gametas, a fertilização artificial do ovócitos, e de incubação dos ovos e larvas de P. lineatus deve ser ~ 7, a fim de promover a criação e produção bem sucedida de animais e de larvas normais.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of water pH on gamete activation, embryonic development, and larval normality in Prochilodus lineatus

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The intracellular pH (pH i ) of steelhead sperm decreases when sperm are exposed to a low‐pH medium (extracellular pH: pH e ; Woosley & Ingermann 2003). It is conceivable that low pH/high CO 2 tensions inhibit sperm motility by suppressing pH i .…”

Section: Discussionmentioning

confidence: 99%

Sperm motility in the steelhead Oncorhynchus mykiss (Walbaum): influence of the composition of the incubation and activation media

et al. 2006

View full text Add to dashboard Cite

This study examined the pH sensitivity of steelhead, Oncorhynchus mykiss (Walbaum), sperm motility relative to the composition of incubation and activation media. The percentage of sperm that initiated motility following incubation in a sperm immobilizing solution (SI) titrated to different pH values and subsequent activation by dilution in buffered swimming medium (SM) at pH 8.5 or 50% ovarian fluid (OF) showed little or no pH sensitivity; sperm diluted in de‐ionized water (DI) showed no motility after incubation at any pH. In contrast, motility of sperm diluted in tap water (TAP) was highly sensitive to the pH of the incubation medium. Sperm incubated with buffered seminal plasma at high, but not low pH demonstrated high percent motility when diluted with DI. Sperm incubated in low‐pH SI demonstrated high motility only when diluted into high‐pH SM. The effects of the composition of incubation and activation media on sperm motility were generally reflected in comparable effects on fertility. Therefore, these data indicate that the pH sensitivity of sperm motility and fertility depends on the composition of commonly used incubation as well as activation media.

show abstract

“…The pH is considered an essential factor in the control of sperm motility (Lahnsteiner et al 1997;Ohta et al 2001b;Tanaka et al 2002a,b;Woolsey and Ingermann 2003;Alavi et al 2004Alavi and Cosson 2005). At 24 hours the motility was zero for all the samples diluted in pH 6.5 media, although the cells were maintained live and any significant changes in the spermatozoa head morphometry was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Possibly, the pH effect had influence in the mechanism of sperm motility, and not in the other parameters. It has been demonstrated that CO 2 inhibits the sperm motility by decreasing the intracellular pH (Morisawa 1994;Woolsey and Ingermann 2003;Cosson 2004;, but the low values of pH could also affect other factors that have influence in the sperm motility. In sea urchin sperm has been demonstrated that ATPase activity is highly dependent on the pH of the medium (Christen et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Study of male reproductive physiology and sperm conservation in the european EEL ( Anguilla anguilla, L.)

Peñaranda¹

View full text Add to dashboard Cite

AGRADECIMIENTOSSon muchos los agradecimientos que tengo que dar y poca la memoria, por lo que pido perdón por adelantado a las personas que no estén incluidas, y agradecerles su apoyo.En primer lugar, quiero aprovechar para decirle a mi familia cuanto les quiero, y darles las gracias por todo lo que han hecho por mí, que no es poco. A mi hermanito, que siempre esta ahí, y no sé que haría sin él. Así que ya sabes, ya estás tardando en volverte para España, que en Irlanda hace mucho frío, je,je. Es broma!!, ya sabes que que lo único importante para mí es que seas feliz.La Mare, que cada día esta peor de la cabeza y por eso cada día le quiero más.Tú confía en mi Mare, que ya verás como algún día cuando pregunten por tus hijos, podrás vacilar de que tienes un hijo biólogo. Je,je, no te enfandes!, que no lo digo en serio, que ya sé que estás muy orgullosa de mí.Por otra parte, y no menos importante el Pare y Trini. Aunque, no siempre todas las épocas han sido buenas, nunca dudeis que os quiero muchísimo, y sólo espero que los malos momentos nunca vuelvan, y en el futuro únicamente podamos hablar de los buenos tiempos.No podría olvidar a mis amigos. Son muchos años los que me habéis aguantado, y aunque yo sé que soy cojonudo y no tengo prácticamente defectos, agradeceros la paciencia que habéis tenido cuando me he puesto un poco borrico, y deciros que pase lo que pase en el futuro siempre podréis contar con un amigo muy noble, pero muy tonto. Así que empezad a temblar (panda de escomendrijos!!!!), que cuando sea doctor habré visto naves en llamas más alla de Orion, y rayos gamma en las puertas de Tamhausen.Quisiera aprovechar para decirles a los Capos una palabra: Gracias.Gracias, por darme la oportunidad de trabajar en lo que considero mi vocación, por lo que me siento una persona muy afortunada. Gracias por todo el esfuerzo realizado para que esta tesis acabe siendo una realidad, y finalmente gracias por brindarme vuestro cariño. Por último, deciros que puede que perdáis un becario, pero a cambio siempre tendréis un amigo (aunque no sé si eso es una buena noticia, je,je..).Es mucha la gente del departamento que esta implicada de una forma u otra en esta tesis. Sería injusto si no hiciera una mención especial a mis dos compañeros y amigos: Javi y Victor. Que independientemente de su apoyo a nivel profesional, lo que siempre he valorado ha sido su amistad sin condiciones.Que sepais, que la cena de degenerados es sagrada, así que iros preparando para hacer unos cuantos montonets en el futuro. También quiero deciros, que aunque no corren los mejores tiempos para los dos, ya podéis ir animándoos porque sino pienso estar dandoos la varilla mucho tiempo, y puedo llegar a ser muy pesao. Un besito muy grande para los dos, pero en plan galletero, que sé que sois muy viciosillos.Tampoco me puedo olvidar de mis otros jefes Miguel Jover, Ana, Andrés y Silvia, último fichaje en las cenas de departamento, y que por suerte no ha aportado la pizca sensatez que se esperaba de ella, y que hace que sean mucho más divertidas. Muchas gra...

show abstract

Acquisition of the potential for sperm motility in steelhead (Oncorhynchus mykiss): effect of pH on dynein ATPase

Cited by 25 publications

References 24 publications

Effects of water pH on gamete activation, embryonic development, and larval normality in Prochilodus lineatus

Effects of water pH on gamete activation, embryonic development, and larval normality in Prochilodus lineatus

Sperm motility in the steelhead Oncorhynchus mykiss (Walbaum): influence of the composition of the incubation and activation media

Study of male reproductive physiology and sperm conservation in the european EEL ( Anguilla anguilla, L.)

Contact Info

Product

Resources

About