Acridine Orange Detection of Plasmodium Falciparum Malaria: Relationship between Sensitivity and Optical Configuration

Long, Gary W.; Jones, Trevor R.; Rickman, LelandS.; Trimmer, Roy W.; Hoffman, S. L.

doi:10.4269/ajtmh.1991.44.402

Cited by 11 publications

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Plasmodium and Babesia

Pritt

2023

ClinMicroNow

View full text Add to dashboard Cite

Plasmodium and Babesia are intraerythrocytic protozoan parasites that cause malaria and babesiosis, respectively. Both are transmitted through the bite of an infected arthropod and cause significant human morbidity and mortality worldwide. The Plasmodium parasite is transmitted primarily through the bite of an infected Anopheles mosquito, and competent mosquito vectors are found throughout the world. The antigens most commonly detected by the commercially available rapid diagnostic tests are pan‐ or species‐specific Plasmodium lactate dehydrogenase, Plasmodium falciparum histidine‐rich protein 2, and panspecific Plasmodium aldolase. Serologic testing plays only a small role in diagnosis of acute malaria, given that antibodies are frequently absent at the time of patient presentation. Members of the genus Babesia infect a wide range of wild and domestic animals worldwide using primarily ixodid tick vectors. Babesiosis due to Babesia microti and Babesia duncani / B. duncani ‐like parasites can manifest as asymptomatic or mild infection to life‐threatening illness.

show abstract

Plasmodium and Babesia

Pritt

2023

ClinMicroNow

View full text Add to dashboard Cite

show abstract

Acridines Used for Staining

Ježek

Hlaváček

Šebestı́k

2017

Progress in Drug Research

View full text Add to dashboard Cite

Diagnosis of malaria in the field by fluorescence microscopy of QBC® capillary tubes

Baird

Purnomo

Jones

1992

Transactions of the Royal Society of Tropical Medicine and Hygi

View full text Add to dashboard Cite

The diagnostic performance of commercial capillary tubes containing acridine orange dye (QBC) was compared with the standard diagnosis of malaria by microscopical examination of Giemsa-stained thick blood films (GTS) in remote field conditions. The comparison was conducted among 165 volunteers living in northeastern Irian Jaya, Indonesia, an area having hyperendemic malaria transmission. By GTS, 65 volunteers were positive for malaria, but only 49 were judged positive by QBC. Among the 100 blood films found negative by GTS, 5 were considered positive by QBC. Thus, relative to a GTS standard, the sensitivity and specificity of the QBC was 75% and 95%, respectively. The mean limit of detection for the QBC was approximately 60 parasites per microliter blood, whereas the limit of detection for GTS was 20 parasites per microliter blood. Also, a number of practical difficulties were encountered using the QBC at the field site. The QBC approach to diagnosis of malaria was less sensitive and more inconvenient than GTS under the conditions in remote Irian Jaya.

show abstract

Acridine Orange Detection of Plasmodium Falciparum Malaria: Relationship between Sensitivity and Optical Configuration

Cited by 11 publications

References 0 publications

Plasmodium and Babesia

Plasmodium and Babesia

Acridines Used for Staining

Diagnosis of malaria in the field by fluorescence microscopy of QBC® capillary tubes

Contact Info

Product

Resources

About