Acridinium chemiluminescence detection with capillary electrophoresis

Ruberto, Michael A.; Grayeski, Mary Lynn

doi:10.1021/ac00046a018

Cited by 85 publications

(63 citation statements)

References 22 publications

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“…Thus, several CL systems including luminol (24−27), peroxyoxalate (28), acridinium (29,30), tris (2,2-bipyridyl) ruthenium(II) (31,32), luciferin-luciferase (33) and permanganate (34) have been developed and coupled to CE for the analysis of various compounds, such as proteins, amino acids, metal ions and so on. Not surprisingly, CL has been considered as a promising detection technique in CE, and has attracted increasing interest (35).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of isoniazid and p‐aminosalicylic acid by capillary electrophoresis using chemiluminescence detection

et al. 2009

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It was found that isoniazid (ISO) or p-aminosalicylic acid (PAS) could enhance the chemiluminescence (CL) emission from Cu (II)-luminol-hydrogen peroxide system, and the increased chemiluminescence signals were proportional to their concentrations, respectively. Based on this phenomenon, a chemiluminescence method coupled to capillary electrophoresis (CE) was established for simultaneous determination of ISO and PAS. The CE conditions including running buffer and running voltage were investigated in detail. The effects of the pH of H 2 O 2 solution and the concentrations of luminol, H 2 O 2 and Cu (II) on the CL signal were also investigated carefully. Under the optimized conditions, the analysis could be accomplished within 10 min, with the limits of detection of 0.3 mg mL -1 for ISO and 1.1 mg mL -1 for PAS, corresponding to 7.2 and 26.4 pg per injection (24 nL), respectively. Finally, the method was validated by determining the two analytes in pharmaceutical preparation and spiked human serum samples. The results of pharmaceutical tablet analysis were in good agreement with the labeled amounts. The recoveries for ISO and PAS in human serum were in the range of 92-104% and 90-113%, respectively.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of isoniazid and p‐aminosalicylic acid by capillary electrophoresis using chemiluminescence detection

et al. 2009

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“…[1][2][3][4] The most commonly used detection mode in CE is UV absorption. More sensitive detection in CE can be achieved with laser-induced fluorescence, 5,6 electrochemical, 7,8 and chemiluminescence (CL) [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] approaches.…”

Section: Introductionmentioning

confidence: 99%

“…29 The coupling of CE-CL offers significantly high sensitivity and selectivity. [30][31][32] To date, a number of CL systems involving luminol, [10][11][12][13][14][15][16][17][18][19][20][21]28 peroxyoxalate, 9,10 permanganate, 24,27 ruthenium(II) complex, 25,26 acridinium esters 23 and firefly luciferase 15 have been utilized for the measurement of dyestuffs, proteins, amino acids, peptides, liposomes, alkaloids, phenols, ATP and metal ions with CE. Two main types of CL detection have been designed for CE.…”

Section: Introductionmentioning

confidence: 99%

“…One is the batch-type [12][13][14][15][16][17]26,27 and the other is the flow-type. [18][19][20][21][22][23][24][25] The batch-type simply immerses the outlet end of a separation capillary into an outlet reservoir containing certain CL reagents and electrolyte buffer. The batch-type provides high sensitivity but is problematic in reproducibility and separation efficiency.…”

Section: Introductionmentioning

confidence: 99%

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On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

Zhang

2007

ANAL. SCI.

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On-capillary chemiluminescence detection for capillary electrophoresis with a single capillary was reported. A hole (about 30 μm diameter) was made on the capillary wall at about 50.5 cm from the inlet end. Hydrogen peroxide solution could enter the capillary from the hole, and mixed with luminol and copper(II) to produce chemiluminescence. The chemiluminescence was detected by a PMT under the hole. Several factors that influenced chemiluminescence intensity were investigated. The detection limits for luminol and N-(4-aminolbutyl)-N-ethylisoluminol (ABEI) were 1 × 10 -11 and 2 × 10 -10 mol L -1 , respectively. The method features simple construction and no dead volume.

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“…acridinium, 10 peroxyoxalate, 11,12 and Ru(II) complex, 13 have been utilized. We have also reported various types of CE with CL detectors, including batch-type [14][15][16] and the flow-type CL detectors.…”

mentioning

confidence: 99%

Analysis of a Biopolymer by Capillary Electrophoresis with a Chemiluminescence Detector Using a Polymer Solution as the Separation Medium

Tsukagoshi¹,

Shikata²,

Nakajima³

et al. 2002

ANAL. SCI.

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Capillary electrophoresis (CE) has been shown to be a powerful and efficient analytical separation technique. The development of sensitive detection methods has become one of the most important subjects in CE studies. Absorption and fluorescence detections are commonly used as on-column optical detection modes, due to an extremely small sample zone and the capillary inner diameter in CE. More sensitive detection can be realized when the analytes can be used in conjunction with laser-induced fluorescence, 1 amperometric, 2 or radiometric 3 detection. An alternative sensitive detection uses chemiluminescence (CL). 4 A CL detection system combined with separation methods can offer excellent analytical selectivity and sensitivity. CL has already been shown to be a highly sensitive detection method in both FIA and HPLC. [5][6][7] Recently, the applicability of CL detection in CE has been successfully demonstrated.Various CL reagents, such as luminol, 8,9 acridinium, 10 peroxyoxalate, 11,12 and Ru(II) complex, 13 have been utilized. We have also reported various types of CE with CL detectors, including batch-type 14-16 and the flow-type CL detectors. 8,11,17 CL detection featured a high sensitivity, a wide determinable range of the sample concentration, and an inexpensive apparatus and reagent.Additionally, a miniaturization of the analytical system must be a predominant merit in a CL detector, because it does not need any light source or spectroscpopy. We could combine microchip CE with a CL detector 18,19 based on the concept of a µ-total analytical system. However, when one takes notice of the separation modes in a CE with CL detector, capillary zone electrophoresis has been used in almost all studies. Only micellar electrokinetic chromatography and capillary isoelectric focusing have been applied as other separation modes in several studies. 20,21 Therefore, although CE with CL detection methods has shown high sensitivity, the methods were not always satisfactory for the separation ability in CE. Capillary gel electrophoresis (CGE) has been recognized as an effective method for the analysis of biopolymers, such as proteins, saccharides, and nucleic acids. A migration buffer solution containing a polymer as the separation medium is also useful and effective, when compared with that using a crosslinked gel immobilized on the inner wall of the capillary. [22][23][24][25] The buffer solution containing the polymer can be easily replaced in the capillary, leading to a reproducible separation of analytes. A separation mode with a buffer solution containing carboxymethylcellulose (CMC) was developed to analyze DNA fragments. 26 We tried for the first time to combine a separation mode using a polymer solution with a CL detector for the analysis of biopolymers. CMC was used as the separation medium. Two post-column CL detectors of batch-and flow-types, both of which were originally developed in the authors' laboratory, were examined. The flow-type CL detector was found to show better resolution than the batch-type for a se...

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Acridinium chemiluminescence detection with capillary electrophoresis

Cited by 85 publications

References 22 publications

Simultaneous determination of isoniazid and p‐aminosalicylic acid by capillary electrophoresis using chemiluminescence detection

Simultaneous determination of isoniazid and p‐aminosalicylic acid by capillary electrophoresis using chemiluminescence detection

On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

Analysis of a Biopolymer by Capillary Electrophoresis with a Chemiluminescence Detector Using a Polymer Solution as the Separation Medium

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