Acridinium ester conjugated to lectin as chemiluminescent histochemistry marker

Abstract

Cell differentiation/dedifferentiation includes changes in oligosaccharide composition and distribution in the cell surface glycoconjugates. Lectins have been used as auxiliary tools in histopathological diagnosis of mammary, uterus and brain pathologies. Acridinium ester (AE) conjugated to biomolecules has been employed in chemiluminescent analytical applications. This work aimed to use a lectin, concanavalin A (Con A), conjugated to AE as a chemiluminescent histochemistry tool. Biopsies of normal and infiltrating duct carcinoma (IDC) of mammary tissues were treated by a Con A-AE derivative. Photon emission, observed during the breakage of the chemical bound between Con A and AE, was quantified, expressed in relative light units (RLU) and correlated to the labelling of the normal and transformed tissues. The results demonstrated that RLU presented a linear relationship with the labelled tissue area in the range 0.125-1.0 cm2 (r=0.98). Furthermore, RLU was much higher for the IDC (1283.920x103+/-220.621x103) than the normal tissue (2.565x103+/-0.247x103), namely, about 500 times higher. The Con A-AE conjugation efficiency, differential staining of normal and IDC tissues, and quantification of results contribute to a decrease in the subjectivity in routine histopathological diagnoses and indicate that acrydinum ester can join other lectin marker to be used in histochemistry.

Glycomic profile of the human parotid gland between 18th and 26th week of fetal development

“…These events can be histochemically characterized through lectin binding to specific terminal or internal sugar sequences of glycoproteins in several tissues (17). Although little is known about the molecular mechanisms that regulate the development of the human salivary gland (18), it is known that variations in the glycosyl components of the cell surface and cytoplasm play a significant role in normal development and pathology (15,19).…”

“…In other words, while carbohydrates carry biological information, lectins act as a communication bridge and allow the interpretation of this "glycocode" (15). Thus, lectins are used in medical and biological fields (e.g., histochemistry), where they can provide a sensitive detection system for changes in glycosylation (16).…”

CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

“…According Campos et al (2006) the processes of cell differentiation and dedifferentiation include changes in the composition of oligosaccharides and their distribution on cell surfaces. On odontogenesis the changes of carbohydrates expression may act as modulators of the dental germs formation process, they can change pivotal functions like ligation pattern of growth factors, as well as the movements of migration and recognition that are involved in tooth formation (Fuenzalida et al, 2000).…”

“…By virtue of their binding specifities, lectins have been used in histochemistry, providing a sensitive detection system for changes in glycosylation and carbohydrate expression that that may occur during embryogenesis, growth and disease (Campos et al, 2006). Tumour lectinology has so far shown cytochemical and histochemical differences between normal and transformed oral tissues including in a single class of tumour (Sobral et al, 2010).…”

Histochemical Localization of Carbohydrates in Morphological Stages of Developing Human Minor Salivary Glands: A Comparative Study with Cytoskeletal Markers

“…In other words, whilst sugars are able to carry the biological information, lectins are capable of translating their code (Kuno et al, 2005). Thus, lectins have been used in the medical and biological areas such as in histochemistry where they can provide a sensitive detection system for changes in glycosylation and carbohydrate expression that may occur during embryogenesis, growth and disease (Campos et al, 2006).…”