Acrolein sensitizes human renal cancer Caki cells to TRAIL-induced apoptosis via ROS-mediated up-regulation of death receptor-5 (DR5) and down-regulation of Bcl-2

Yang, Eun Sun; Woo, Seon Min; Choi, Kyeong Sook; Kwon, Taeg Kyu

doi:10.1016/j.yexcr.2011.08.005

Cited by 22 publications

(13 citation statements)

References 43 publications

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“…Increased oxidative stress is involved in the apoptotic response induced by some anti-cancer agents (Luo et al, 2010;Yang et al, 2011). Zhang et al (Zhang et al, 2013) reported that AD-1, a novel ginsenoside derivative, exhibited anti-lung cancer property by activating the p38 MAPK pathway and by inducing reactive oxygen species generation.…”

Section: Discussionmentioning

confidence: 99%

12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis

Wang

Sun

Zhao

et al. 2017

European Journal of Pharmacology

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(20R)-Dammarane-3β, 12β, 20, 25-tetrol (25-OH-PPD) is a ginsenoside isolated from Panax ginseng (C. A. Meyer). This compound exhibits anti-cancer activities on many human cancer cell lines. In this study, we investigated anti-cancer mechanisms of 12β-O-(-Chloracetyl)-dammar-20(22)-ene-3β,25-diol(12-Chloracetyl-PPD), a modified 25-OH-PPD. We found that compound 12-Chloracetyl-PPD resulted in a concentration-dependent inhibition of viability in prostate, breast, and gastric cancer cells, without affecting the viability of normal cell (human gastric epithelial cell line-GES-1, hair follicle dermal papilla cell line-HHDPC and rat myocardial cell line-H9C2). In MDA-MB-435 and C4-2B cancer cells, 12-Chloracetyl-PPD induced G2/M cell cycle arrest, down-regulated mouse double minute 2 (MDM2) expression, up-regulated p53 expression, triggered apoptosis, and stimulated reactive oxygen species production. Apoptosis can be attenuated by the reactive oxygen species scavenger N-acetylcysteine. Our results suggested that compound 12-Chloracetyl-PPD showed obvious anti-cancer activity based on delaying cell cycle arrest and inducing cell apoptosis by reactive oxygen species production, which supported development of 12-Chloracetyl-PPD as a potential agent for cancer chemotherapy.

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Section: Discussionmentioning

confidence: 99%

12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis

Wang

Sun

Zhao

et al. 2017

European Journal of Pharmacology

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“…In addition, the combined treatment with rosiglitazone and TNF-α-related apoptosis inducing ligand (TRAIL) could induce apoptosis in renal cancer cells via induction of Bcl-2 overexpression [ 26 ]. Similarly acrolein can effectively sensitize human renal Caki cells to TRAIL-induced apoptosis through down-regulating Bcl-2 and up-regulating DR5 , mediated via generation of reactive oxygen species and induction of the C/EBP homologous protein [ 27 ]. Thus, lowering the TRAIL resistance or increasing the damage of tumor cells could help in cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, lowering the TRAIL resistance or increasing the damage of tumor cells could help in cancer therapy. Moreover, the knockdown of MADD and c-FLIP reduced the resistance to TRAIL-induced apoptosis in SKOV-3 ovarian cancer cells to 64.2 ± 3.0 % [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Cardanol isolated from Thai Apis mellifera propolis induces cell cycle arrest and apoptosis of BT-474 breast cancer cells via p21 upregulation

et al. 2015

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BackgroundCardanol was previously reported to be an antiproliferative compound purified from Thai Apis mellifera propolis. By morphology, it could induce the cell death to many cancer cell lines but not the control (non-transformed human foreskin fibroblast cell line, Hs27). Here, it was aimed to evaluate the molecular effects of cardanol on breast cancer derived cell line (BT-474).MethodsMorphological changes in BT-474 cells induced by cardanol compared to doxorubicin were evaluated by light microscopy, cytotoxicity by using the 3- (4, 5-dimethyl-thiazol-2-yl) 2, 5-diphenyl-tetrazolium bromide (MTT) assay, induction of cell cycle arrest and cell death by flow cytometric analysis of propidium iodide and annexin-V stained cells, and changes in the expression level of genes involved in the control of apoptosis and the cell cycle by quantitative reverse transcriptase-PCR (qRT-PCR) and western blot analyses.ResultsIt revealed that cardanol induced a time- and dose-dependent cytotoxicity along with cell shrinkage and detachment from substratum. Cardanol caused cell cycle arrest at the G1 subphase (as opposed to at the G2/M subphase seen with doxorubicin) and cell death by late apoptosis, with both late apoptosis (27.2 ± 1.1 %) and necrosis (25.4 ± 1.4 %) being found in cardanol treated cells after 72 h, compared to a lower proportion of apoptosis (4.3 ± 0.4 %) and higher proportion of necrosis (35.8 ± 13.0 %) induced by doxorubicin. Moreover, cardanol changed the transcript expression levels of genes involved in the control of apoptosis (increased DR5 and Bcl-2 expression and decreased Mcl-1, MADD and c-FLIPP) and cell division (increased p21 and E2FI and decreased cyclin D1, cyclin E, CDK4 and CDK2 expression), as well as increasing the level of p21 p-ERK, p-JNK and p-p38 and decreasing cyclin D. This accounts for the failure to progress from the G1 to the S subphase.ConclusionCardanol is a potential chemotherapeutic agent for breast cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s40199-015-0138-1) contains supplementary material, which is available to authorized users.

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“…A COPD model mouse has been established by the use of ACR [ 7 ] or smoking [ 12 , 30 ]. ACR has also been used to create cell death models in various cell lines [ 31 , 34 , 47 , 50 ]. The mechanism of ACR-induced cell death, including apoptosis, has been studied in the human alveolar adenocarcinoma cell line A549 [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

SCGB3A2 Inhibits Acrolein-Induced Apoptosis through Decreased p53 Phosphorylation

Kurotani

Shima

Miyano

et al. 2015

Acta Histochemica et Cytochemica

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Chronic obstructive pulmonary disease (COPD), a major global health problem with increasing morbidity and mortality rates, is anticipated to become the third leading cause of death worldwide by 2020. COPD arises from exposure to cigarette smoke. Acrolein, which is contained in cigarette smoke, is the most important risk factor for COPD. It causes lung injury through altering apoptosis and causes inflammation by augmenting p53 phosphorylation and producing reactive oxygen species (ROS). Secretoglobin (SCGB) 3A2, a secretory protein predominantly present in the epithelial cells of the lungs and trachea, is a cytokine-like small molecule having anti-inflammatory, antifibrotic, and growth factor activities. In this study, the effect of SCGB3A2 on acrolein-related apoptosis was investigated using the mouse fibroblast cell line MLg as the first step in determining the possible therapeutic value of SCGB3A2 in COPD. Acrolein increased the production of ROS and phosphorylation of p53 and induced apoptosis in MLg cells. While the extent of ROS production induced by acrolein was not affected by SCGB3A2, p53 phosphorylation was significantly decreased by SCGB3A2. These results demonstrate that SCGB3A2 inhibited acrolein-induced apoptosis through decreased p53 phosphorylation, not altered ROS levels.

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Acrolein sensitizes human renal cancer Caki cells to TRAIL-induced apoptosis via ROS-mediated up-regulation of death receptor-5 (DR5) and down-regulation of Bcl-2

Cited by 22 publications

References 43 publications

12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis

12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis

Cardanol isolated from Thai Apis mellifera propolis induces cell cycle arrest and apoptosis of BT-474 breast cancer cells via p21 upregulation

SCGB3A2 Inhibits Acrolein-Induced Apoptosis through Decreased p53 Phosphorylation

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