Acrosin Profiles of Human Spermatozoa Recovered from the New Sperm Prep II Filtration Column.

Sofikitis, Nikolaos; Miyagawa, Ikuo; Zavos, Panayiotis M.; Inaga, Sumire; Iino, Akihiro; Toda, Toshiko; Harada, Tasuku; Mio, Y.; Terakawa, Naoki

doi:10.1620/tjem.166.451

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…Further in comparison with swim‐up and glass wool filtration, Sephadex ™ filtration does not involve centrifugation which provides additional advantage of reducing oxidative stress damages to the sperm. It is relevant to mention that filtration yielded sperm with higher acrosin activity in human (Sofikitis et al., ). Therefore, the increased fertilization rate using post‐thaw Sephadex‐filtered semen in our study may be due to increased proportion of motile, intact, viable and live sperm having higher acrosin content thereby providing more competent spermatozoa for fertilization.…”

Section: Discussionmentioning

confidence: 99%

Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes

Husna

Azam

Qadeer

et al. 2017

Reprod Domestic Animals

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Routinely, swim-up method is used to separate high-quality sperm; however, long processing time and close cell-to-cell contact during the centrifugation step are inevitable elements of oxidative stress to sperm. The objective was to evaluate Sephadex and glass wool filtration to separate motile, intact and viable sperm for in vitro fertilization in buffalo. The cumulus-oocyte complexes (COCs) were collected from ovaries of slaughtered buffaloes by aspiration and matured for 24 hr in CO incubator at 38.5°C and 5% CO . Matured COCs were rinsed twice in fertilization TALP and placed in the pre-warmed fertilization medium without sperm. Cryopreserved buffalo semen was thawed at 37°C for 30 s and processed through Sephadex , glass wool filtration and swim-up (control). Total and motile sperm recovery rates were assessed, resuspended in fertilization TALP and incubated for 15-20 min in CO incubator. Samples prepared by each method were divided into two aliquots: one aliquot was studied for sperm quality (progressive motility, membrane integrity, viability, liveability), while the other was subjected to co-incubation with sets of 10-15 in vitro matured oocytes. Data on sperm quality were analysed by ANOVA, while in vitro fertilizing rates were compared by chi-squared test using SPSS-20. Least significant difference (LSD) test was used to compare treatment means. Glass wool filtration yielded higher total and motile sperm recovery rate, while Sephadex filtration improved (p < .05) sperm quality (progressive motility, membrane integrity, viability, liveability). Sperm preparation through Sephadex filtration yielded higher in vitro fertilization rate in terms of cleavage rate compared to glass wool filtration and swim-up (control). In conclusion, cryopreserved Nili-Ravi buffalo sperm selected through Sephadex filtration showed improved quality and yielded better fertilization rates (cleavage rate) of in vitro matured/fertilized oocytes. Sephadex filtration could be a promising technique for use in in vitro fertilization in buffalo.

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Section: Discussionmentioning

confidence: 99%

Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes

Husna

Azam

Qadeer

et al. 2017

Reprod Domestic Animals

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“…Excessive ROS has also been associated with sperm DNA fragmentation (SDF), which may mediate the clinical manifestation of poor sperm function and infertility related to varicocele[4,7,8,18,25-27]. A significantly less total acrosin activity in the spermatozoa of infertile men with varicocele and an abnormal retention of cytoplasmic droplets by human spermatozoa, which is negatively correlated with sperm motility, are other potential contributing factors for the diminished sperm function in individuals with varicocele[4,28]. …”

Section: Pathophysiologymentioning

confidence: 99%

Potential role of imaging in assessing harmful effects on spermatogenesis in adult testes with varicocele

Tsili

Xiropotamou

Sylakos

et al. 2017

WJR

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Varicocele is characterized by an abnormal dilatation and retrograde blood flow in the spermatic veins. Varicocele is the leading correctable cause of male infertility. Although it is highly prevalent in infertile men, it is also observed in individuals with normal fertility. Determining which men are negatively affected by varicocele would enable clinicians to better select those men who will benefit from treatment. To assess the functional status of the testes in men with varicocele, color Doppler sonographic parameters were evaluated. Testicular arterial blood flow was significantly reduced in men with varicocele, reflecting an impairment of spermatogenesis. An improvement in the testicular blood supply was found after varicocelectomy on spectral Doppler analysis. Testicular contrast harmonic imaging and elastography might improve our knowledge about the influence of varicocele on intratesticular microcirculation and tissue stiffness, respectively, providing possible information on the early damage of testicular structure by varicocele. Magnetic resonance imaging (MRI), with measurement of apparent diffusion coefficient has been used to assess the degree of testicular dysfunction and to evaluate the effectiveness of varicocele repair. Large prospective studies are needed to validate the possible role of functional sonography and MRI in the assessment of early defects of spermatogenesis in testes with varicocele.

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“…Moreover, toxic effects are enhanced in the presence of egg yolk (Romanoff and Romanoff, 1949), whose concentration might have decreased after filtration reducing toxin production. Sofikitis et al (1992) reported that the filtration of semen yielded sperm with higher acrosin activity. So, the increased CR using post thaw Sephadex filtered semen may be due to increased proportion of motile, live sperm having higher acrosin content thereby providing more competent spermatozoa for fertilization.…”

Section: Fertility Trialmentioning

confidence: 99%

Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen

Kumar¹,

Singh²,

Nanda³

et al. 2004

Asian Australas. J. Anim. Sci

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The present investigation was carried out to assess the effect of Sephadex (G-15) filtration on the post thaw bull semen quality and conception rate. Post thaw unfiltered (control) and Sephadex filtered semen from four healthy bulls (three cross bred and one pure bred Holstein Friesian) were subjected to microscopic examination viz. sperm concentration, individual motility, live sperm count and sperm morphology. Sixty-two healthy, normal cycling crossbred cows were inseminated with post thaw unfiltered (n=32) and filtered semen (n=30). Sephadex filtration of post thaw semen significantly (p<0.05) decreased total sperm concentration and sperm with abnormal head, mid piece and tail. The overall average total sperm concentration, head and tail defects in filtered semen decreased significantly (53.4, 1.2 and 6.4 million) than in the unfiltered semen (80.4, 2.4 and 15.7 million, respectively). However, after filtration significant (p<0.05) increase in overall average motile and live sperm concentration were observed (38.8 and 38.0) as compared to unfiltered semen (29.2 and 32.0 million, respectively). The overall conception rate recorded was 21.9% with post thaw unfiltered semen and 56.7% with filtered semen. It was concluded that Sephadex filtration of post thaw semen improved its quality and conception rate.

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Acrosin Profiles of Human Spermatozoa Recovered from the New Sperm Prep II Filtration Column.

Cited by 10 publications

References 11 publications

Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes

Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes

Potential role of imaging in assessing harmful effects on spermatogenesis in adult testes with varicocele

Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen

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Acrosin Profiles of Human Spermatozoa Recovered from the New Sperm Prep II Filtration Column.

Cited by 10 publications

References 11 publications

Sperm preparation through Sephadex™ filtration improves in vitro fertilization rate of buffalo oocytes

Sperm preparation through Sephadex™ filtration improves in vitro fertilization rate of buffalo oocytes

Potential role of imaging in assessing harmful effects on spermatogenesis in adult testes with varicocele

Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen

Contact Info

Product

Resources

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Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes

Sperm preparation through Sephadex^™ filtration improves in vitro fertilization rate of buffalo oocytes