Acrosomal integrity, viability, and DNA damage of sperm from dasyurid marsupials after freezing or freeze drying

Czarny, N. A.; Harris, M. S.; Iuliis, Geoffry N. De; Rodger, John C.

doi:10.1016/j.theriogenology.2009.05.018

Cited by 20 publications

(2 citation statements)

References 28 publications

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“…mice, boar) while others require concentrations as high as 28% for freezing to be successful, with even higher concentrations to maintain high DNA integrity (e.g. in marsupials: Johnston et al, 1993;Czarny et al, 2009a). In some cases insemination can be done with the thawed sample [e.g.…”

Section: Semen Freezingmentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Section: Semen Freezingmentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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“…So far, freeze-dried spermatozoa have been used to generate embryos from a wide range of species, including mice (Wakayama & Yanagimachi 1998), rats (Hirabayashi et al 2005), rabbits (Liu et al 2004), dogs (Watanabe et al 2009), cattle (Martins et al 2007, Abdalla et al 2009a, 2009b, pigs (Nakai et al 2007), primates (Sánchez-Partida et al 2008), marsupials (Czarny et al 2009), humans (Kusakabe et al 2008), and fish (Poleo et al 2005). Although most of these papers do not report the birth of animals from freezedried spermatozoa, they indicate the wide interest on alternative storage methods for spermatozoa, including human sperm (A Arav 2011, personal communication).…”

Section: Introductionmentioning

confidence: 99%

Genome of non-living cells: trash or recycle?

Loi

Fulka²,

Hildebrand³

et al. 2011

Reproduction

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Reproductive technologies have been often used as a tool in research not strictly connected with developmental biology. In this study, we retrace the experimental routes that have led to the adoption of two reproductive technologies, ICSI and somatic cell nuclear transfer (SCNT), as biological assays to probe the 'functionality' of the genome from dead cells. The structural peculiarities of the spermatozoa nucleus, namely its lower water content and its compact chromatin structure, have made it the preferred cell for these experiments. The studies, primarily focused on mice, have demonstrated an unexpected stability of the spermatozoa nuclei, which retained the capacity to form pronuclei once injected into the oocytes even after severe denaturing agents like acid treatment and high-temperature exposure. These findings inspired further research culminating in the production of mice after ICSI of lyophilized spermatozoa. The demonstrated non-equivalence between cell vitality and nuclear vitality in spermatozoa prompted analogous studies on somatic cells. Somatic cells were treated with the same physical stress applied to spermatozoa and were injected into enucleated sheep oocytes. Despite the presumptive fragile nuclear structure, nuclei from non-viable cells (heat treated) directed early and post-implantation embryonic development on nuclear transfer, resulting in normal offspring. Recently, lyophilized somatic cells used for nuclear transfer have developed into normal embryos. In summary, ICSI and SCNT have been useful tools to prove that alternative strategies for storing banks of non-viable cells are realistic. Finally, the potential application of freeze-dried spermatozoa and cells is also discussed.

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Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

Comizzoli

Amelkina

Lee

2022

Molecular Reproduction Devel

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Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures has the potential to lower the costs and simplify biobanking in human reproductive medicine, as well as for the management of animal populations. Over the past decades, different dehydration protocols and long‐term storage solutions at nonfreezing temperatures have been explored, mainly for mammalian sperm cells. Oocytes and gonadal tissues are more challenging to dehydrate so little to no progress have been made. Currently, the detrimental effects of the drying process itself are better characterized than the impact of long‐term storage at nonfreezing temperatures. While structural and functional properties of germ cells can be preserved after dehydration, a long list of damages and stresses in nuclei, organelles, and cytoplasmic membranes have been reported and sometimes mitigated. Characterizing those damages and better understanding the response of germ cells and tissues to the stress of dehydration is fundamental. It will contribute to the development of optimal protocols while proving the safety of alternative storage options for fertility preservation. The objective of this review is to (1) document the types of damages and stress responses, as well as their mitigation in cells dried with different techniques, and (2) propose new research directions.

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Acrosomal integrity, viability, and DNA damage of sperm from dasyurid marsupials after freezing or freeze drying

Cited by 20 publications

References 28 publications

Genome Banking for Vertebrates Wildlife Conservation

Genome Banking for Vertebrates Wildlife Conservation

Genome of non-living cells: trash or recycle?

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

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