Acrosome Breakdown in Leptodactylus Chaquensis (Amphibia Anura) Spermatozoa*

Raisman, Jorge S.; Cunio, Rita W. De; Cabada, Marcelo O.; Pino, Enrique J. del; Mariano, Marta I.

doi:10.1111/j.1440-169x.1980.00289.x

Cited by 38 publications

(20 citation statements)

References 31 publications

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“…A necessary condition for spermatozoa to fertilize oocytes is to reach the vitelline envelope with the acrosome intact, or at least not completely reacted. It has been shown in Leptodactylus chaquensis that spermatozoa with intact acrosomes can reach the vitelline envelope of homologous oocyte (Raisman et al, 1980). A study of the relative roles of jelly layers in fertilization of Bufo japonicus (Omata, 1993) also confirms the previous report by Yoshizaki and Katagiri (1982) that acrosome reaction occurs not in the jelly but at or near the surface of the vitelline coat of fully jellied oocytes.…”

Section: Introductionsupporting

confidence: 85%

Diffusible highly glycosylated protein from Bufo arenarum egg‐jelly coat: Biological activity

Arranz

Cabada

2000

Mol. Reprod. Dev.

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L-HGP is a highly glycosylated protein from Bufo arenarum egg-jelly coat that diffuses into the surrounding medium when the strings of oocytes are incubated in saline solutions. L-HGP was purified from egg water and the estimated percentage of L-HGP/total protein in egg water was estimated in 30%. In the present study we examine, by indirect immunofluorescence, the effect of L-HGP on acrosome status of homologous spermatozoa. A high percentage (77%) of sperm lost the acrosome when incubated in 10% Ringer solution buffered with 10 mM Tris-HCl, pH 7.6, during 60 min, a condition that resembles egg-jelly osmolarity. The addition of purified L-HGP to the incubation medium prevents acrosome breakdown. The acrosome integrity is maintained for at least 1 hr. This effect is specific for L-HGP at concentration ranging from 0.01 to 0.1 mg/ml since neither BSA nor fetuin seems to have similar activity at similar concentrations. The same effect was observed when spermatozoa were incubated in egg water. Preliminary results suggest that L-HGP binds to B. arenarum spermatozoan membranes.

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Section: Introductionsupporting

confidence: 85%

Diffusible highly glycosylated protein from Bufo arenarum egg‐jelly coat: Biological activity

Arranz

Cabada

2000

Mol. Reprod. Dev.

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“…However, in D. pictus, the acrosome reaction takes place in the jelly coat layers (Campanella et al, 1997). In X. laevis, B. japonicus, B. arenarum, and L. chaquensis the majority of sperm bound to the VE have intact acrosomes (Martinez and Cabada, 1996;Raisman et al, 1980;Ueda et al, 2003;Yoshizaki and Katagiri, 1982). Thus, nonenvelope factors are responsible for induction of the acrosome reaction.…”

Section: Sperm Acrosome Reaction and Penetrationmentioning

confidence: 99%

Anuran and pig egg zona pellucida glycoproteins in fertilization and early development

Hedrick

2008

Int. J. Dev. Biol.

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The envelope surrounding the eggs of all animals has many biological functions in fertilization and development. This review focuses on the anuran egg envelope in terms of its biochemistry, biophysics, structural biology and function in sperm-egg interactions and early development (embryo hatching). Egg envelopes from Xenopus laevis are among the most studied of the anurans, and are the central theme of this review. Comparisons of Xenopus laevis envelopes with those of other anurans and with pig egg envelopes are also included. This article presents historical as well as contemporary comparative perspectives on molecular and cellular mechanisms of sperm-egg envelope binding, block to polyspermy, envelope hardening, and hatching.

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“…Isotonicity helps maintain both acrosome integrity and sperm viability (Arranz and Cabada, 2000; Cabada, 1975). Dilution into hypotonic environments at either spawning or gamete handling activates flagellar motility rendering sperm capable of reaching the egg (Raisman, 1980). EW, a complex solution composed by oviduct derived ions and glucolipoproteic factors, mimics natural hypotonic medium for Bufo arenarum fertilization, where sperm acquire the capacity to undergo agonist stimulated acrosome reaction (Krapf et al, 2009) and fertilize the egg (Krapf et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation

O’Brien

Krapf

Cabada

et al. 2011

Developmental Biology

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Sperm motility is essential for achieving fertilization. In animals with external fertilization as amphibians, spermatozoa are stored in a quiescent state in the testis. Spermiation to hypotonic fertilization media triggers activation of sperm motility. Bufo arenarum sperm are immotile in artificial seminal plasma (ASP) but acquire in situ flagellar beating upon dilution. In addition to the effect of low osmolarity on sperm motility activation, we report that diffusible factors of the egg jelly coat (EW) regulate motility patterns, switching from in situ to progressive movement. The signal transduction pathway involved in amphibian sperm motility activation is mostly unknown. In the present study, we show a correlation between motility activation triggered by low osmotic pressure and activation of protein kinase A (PKA). Moreover, this is the first study to present strong evidences that point toward a role of a transmembrane adenyl-cyclase (tmAC) in the regulation of amphibian sperm motility through PKA activation.

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Acrosome Breakdown in Leptodactylus Chaquensis (Amphibia Anura) Spermatozoa*

Cited by 38 publications

References 31 publications

Diffusible highly glycosylated protein from Bufo arenarum egg‐jelly coat: Biological activity

Diffusible highly glycosylated protein from Bufo arenarum egg‐jelly coat: Biological activity

Anuran and pig egg zona pellucida glycoproteins in fertilization and early development

Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation

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