Acrosome Reaction in Fertile and Subfertile Boar Sperm

Herrera, Julián A.; Fierro, Reyna; Zayas, Héctor; Conejo, J; Jiménez, Irma; García, A. R.; Betancourt, Miguel

doi:10.1080/014850102317267445

Cited by 15 publications

(11 citation statements)

References 14 publications

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“…Male subfertility is also present in other species besides cattle [9], [48]–[50], and compromised sperm surface proteins account for a substantial number of males with distinctly reduced reproductive ability in humans [15], [40]. Our results demonstrate that TMEM95 is another sperm surface protein, which is likely to be involved in sperm-egg plasma membrane interactions.…”

Section: Discussionmentioning

confidence: 60%

A Nonsense Mutation in TMEM95 Encoding a Nondescript Transmembrane Protein Causes Idiopathic Male Subfertility in Cattle

Pausch¹,

Kölle

Wurmser³

et al. 2014

PLoS Genet

145

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Genetic variants underlying reduced male reproductive performance have been identified in humans and model organisms, most of them compromising semen quality. Occasionally, male fertility is severely compromised although semen analysis remains without any apparent pathological findings (i.e., idiopathic subfertility). Artificial insemination (AI) in most cattle populations requires close examination of all ejaculates before insemination. Although anomalous ejaculates are rejected, insemination success varies considerably among AI bulls. In an attempt to identify genetic causes of such variation, we undertook a genome-wide association study (GWAS). Imputed genotypes of 652,856 SNPs were available for 7962 AI bulls of the Fleckvieh (FV) population. Male reproductive ability (MRA) was assessed based on 15.3 million artificial inseminations. The GWAS uncovered a strong association signal on bovine chromosome 19 (P = 4.08×10−59). Subsequent autozygosity mapping revealed a common 1386 kb segment of extended homozygosity in 40 bulls with exceptionally poor reproductive performance. Only 1.7% of 35,671 inseminations with semen samples of those bulls were successful. None of the bulls with normal reproductive performance was homozygous, indicating recessive inheritance. Exploiting whole-genome re-sequencing data of 43 animals revealed a candidate causal nonsense mutation (rs378652941, c.483C>A, p.Cys161X) in the transmembrane protein 95 encoding gene TMEM95 which was subsequently validated in 1990 AI bulls. Immunohistochemical investigations evidenced that TMEM95 is located at the surface of spermatozoa of fertile animals whereas it is absent in spermatozoa of subfertile animals. These findings imply that integrity of TMEM95 is required for an undisturbed fertilisation. Our results demonstrate that deficiency of TMEM95 severely compromises male reproductive performance in cattle and reveal for the first time a phenotypic effect associated with genomic variation in TMEM95.

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Section: Discussionmentioning

confidence: 60%

A Nonsense Mutation in TMEM95 Encoding a Nondescript Transmembrane Protein Causes Idiopathic Male Subfertility in Cattle

Pausch¹,

Kölle

Wurmser³

et al. 2014

PLoS Genet

145

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“…After capacitation, these residues decreased in both groups, but were unchanged after AR in the subfertile group. In a parallel study using the same samples, the percentage of acrosome-reacted sperm was lower in subfertile boars [29]. This ® nding suggests that FI stay constant in subfertile boars samples because sperm failed to perform AR.…”

Section: Expressi On Of Lectin Receptor S In Boars 163mentioning

confidence: 58%

Expression of Lectin Receptors on the Membrane Surface of Sperm of Fertile and Subfertile Boars by Flow Cytometry

Jiménez

González‐Márquez

Ortiz

et al. 2002

Archives of Andrology

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Studies suggest that carbohydrates are important in different stages of fertilization. Plasma membrane changes accompanying in vitro capacitation and acrosome reaction (AR), such as removal or appearance of specific glycoproteins, have been studied using lectins that bind specifically to carbohydrate residues. In specialized artificial insemination farms and semen production centers, identification of boars with decreased fertilization ability (subfertility) is a newborn necessity. This investigation is a sequential study to determine the kinetics of surface carbohydrates turnover during in vitro capacitation and AR in fertile and subfertile boar sperm. Flow cytometry determinations of the binding of three FITC-labeled lectins were assessed. WGA binding was significantly lower in fresh, capacitated, and acrosome-reacted sperm of subfertile boars than in fertile boars. Con-A binding was not significantly different in fresh sperm of fertile and subfertile boars. However. Con-A labeling in capacitated, and acrosome-reacted sperm differed significantly in both groups. UEA binding increased only in capacitated sperm of subfertile boars. These findings could be used as indicators of capacitation and AR and may also be a good indicator of sperm fertilizing ability in boars.

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“…The inability of spermatozoa to undergo the AR affects the male fertilizing potential under both in vivo and in vitro conditions, and may play a decisive role in unexplained low fertility (Birck et al, 2010). It has been previously demonstrated in several species that the percentage of the in vitro-induced AR is significantly lower in subfertile animals compared with fertile animals (Herrera et al, 2002;Meyers et al, 1996;Whitfield and Parkinson, 1992). For this reason, the study of this trait becomes important for high genetic value animals.…”

Section: Discussionmentioning

confidence: 97%

In vitro induction of the acrosome reaction in spermatozoa from endangered Spanish bulls: Effect of breed, culture media and incubation time

et al. 2012

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Acrosome Reaction in Fertile and Subfertile Boar Sperm

Cited by 15 publications

References 14 publications

A Nonsense Mutation in TMEM95 Encoding a Nondescript Transmembrane Protein Causes Idiopathic Male Subfertility in Cattle

A Nonsense Mutation in TMEM95 Encoding a Nondescript Transmembrane Protein Causes Idiopathic Male Subfertility in Cattle

Expression of Lectin Receptors on the Membrane Surface of Sperm of Fertile and Subfertile Boars by Flow Cytometry

In vitro induction of the acrosome reaction in spermatozoa from endangered Spanish bulls: Effect of breed, culture media and incubation time

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