Acrylamide alters neurotransmitter induced calcium responses in murine ESC-derived and primary neurons

Sisnaiske, Julia; Hausherr, Vanessa; Krug, Anne; Zimmer, Bastian; Hengstler, Jan G.; Leist, Marcel; Thriel, Christoph van

doi:10.1016/j.neuro.2014.03.010

Cited by 32 publications

(13 citation statements)

References 61 publications

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“…A recent in vitro study 35 reported that non-cytotoxic concentrations of acrylamide alter neurotransmitter induced calcium responses in murine ESC-derived and primary neurons. In addition, acrylamide can damage the blood-cerebro spinal fluid barrier and impairs secretory and transport functions.…”

Section: Discussionmentioning

confidence: 99%

Dietary acrylamide exposure was associated with mild cognition decline among non-smoking Chinese elderly men

Liu

Tse

Chen

et al. 2017

Sci Rep

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The aim of the study is to explore the longitudinal association of dietary acrylamide exposure with cognitive performance in Chinese elderly. The analysis was conducted among 2534 non-smoking elderly men and women based on a prospective study, Mr. and Ms. OS Hong Kong. Dietary acrylamide intake was assessed by food frequency questionnaires with data on local food contamination, derived from the first Hong Kong Total Diet Study. Global cognitive function was assessed by Cantonese version of Mini-Mental State Exam (MMSE) at the baseline and the 4th year of follow-up. Multivariable-adjusted linear and logistic regression models were used to assess the associations of dietary acrylamide with MMSE score changes or risk of poor cognition. The results indicated that among men with MMSE ≥ 18, each one SD increase of acrylamide decreased MMSE score by 7.698% (95%CI: −14.943%, −0.452%; p = 0.037). Logistic regression revealed an increased risk of poor cognition (MMSE ≤ 26) in men with HR of 3.356 (1.064~10.591, p = 0.039). The association became non-significance after further adjustment for telomere length. No significant association was observed in women. Dietary acrylamide exposure was associated with a mild cognitive decline or increased risk of poor cognition over a 4-year period in non-smoking Chinese elderly men.

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Section: Discussionmentioning

confidence: 99%

Dietary acrylamide exposure was associated with mild cognition decline among non-smoking Chinese elderly men

Liu

Tse

Chen

et al. 2017

Sci Rep

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“…These in vitro systems recapitulate the critical phases of development, during which they are exposed to test compounds (Krug et al 2013). Meanwhile, these systems have been applied in numerous studies to identify and characterize developmental toxicants (Balmer et al 2014; Meganathan et al 2012, 2015; Sisnaiske et al 2014; Zimmer et al 2014). Genome-wide expression studies have been performed to classify developmental toxicants (Rempel et al 2015), and an eight-gene classifier has been shown to distinguish compounds acting as histone deacetylase inhibitors (HDACis) from a heterogeneous group of ‘mercurials’.…”

Section: Introductionmentioning

confidence: 99%

Definition of transcriptome-based indices for quantitative characterization of chemically disturbed stem cell development: introduction of the STOP-Toxukn and STOP-Toxukk tests

et al. 2016

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Stem cell-based in vitro test systems can recapitulate specific phases of human development. In the UKK test system, human pluripotent stem cells (hPSCs) randomly differentiate into cells of the three germ layers and their derivatives. In the UKN1 test system, hPSCs differentiate into early neural precursor cells. During the normal differentiation period (14 days) of the UKK system, 570 genes [849 probe sets (PSs)] were regulated >fivefold; in the UKN1 system (6 days), 879 genes (1238 PSs) were regulated. We refer to these genes as ‘developmental genes’. In the present study, we used genome-wide expression data of 12 test substances in the UKK and UKN1 test systems to understand the basic principles of how chemicals interfere with the spontaneous transcriptional development in both test systems. The set of test compounds included six histone deacetylase inhibitors (HDACis), six mercury-containing compounds (‘mercurials’) and thalidomide. All compounds were tested at the maximum non-cytotoxic concentration, while valproic acid and thalidomide were additionally tested over a wide range of concentrations. In total, 242 genes (252 PSs) in the UKK test system and 793 genes (1092 PSs) in the UKN1 test system were deregulated by the 12 test compounds. We identified sets of ‘diagnostic genes’ appropriate for the identification of the influence of HDACis or mercurials. Test compounds that interfered with the expression of developmental genes usually antagonized their spontaneous development, meaning that up-regulated developmental genes were suppressed and developmental genes whose expression normally decreases were induced. The fraction of compromised developmental genes varied widely between the test compounds, and it reached up to 60 %. To quantitatively describe disturbed development on a genome-wide basis, we recommend a concept of two indices, ‘developmental potency’ (D p) and ‘developmental index’ (D i), whereby D p is the fraction of all developmental genes that are up- or down-regulated by a test compound, and D i is the ratio of overrepresentation of developmental genes among all genes deregulated by a test compound. The use of D i makes hazard identification more sensitive because some compounds compromise the expression of only a relatively small number of genes but have a high propensity to deregulate developmental genes specifically, resulting in a low D p but a high D i. In conclusion, the concept based on the indices D p and D i offers the possibility to quantitatively express the propensity of test compounds to interfere with normal development.Electronic supplementary materialThe online version of this article (doi:10.1007/s00204-016-1741-8) contains supplementary material, which is available to authorized users.

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“…To confirm whether the nerve cells cultured from the trigeminal ganglion explants were healthy, we performed in vitro Ca 2+ imaging using intracellular Ca 2+ indicator Cal‐520. It is well known that high potassium conditions lead to an increase in Ca 2+ concentration in cultured nerve cells (Sisnaiske et al., ; Yao, Zhao, Liu, Chow, & Mei, ). In the basal K + condition, no increase in Ca 2+ concentration was detected (Video ).…”

Section: Resultsmentioning

confidence: 99%

In vitro formation of the Merkel cell‐neurite complex in embryonic mouse whiskers using organotypic co‐cultures

2018

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A Merkel cell-neurite complex is a touch receptor composed of specialized epithelial cells named Merkel cells and peripheral sensory nerves in the skin. Merkel cells are found in touch-sensitive skin components including whisker follicles. The nerve fibers that innervate Merkel cells of a whisker follicle extend from the maxillary branch of the trigeminal ganglion. Whiskers as a sensory organ attribute to the complicated architecture of the Merkel cell-neurite complex, and therefore it is intriguing how the structure is formed. However, observing the dynamic process of the formation of a Merkel cell-neurite complex in whiskers during embryonic development is still difficult. In this study, we tried to develop an organotypic co-culture method of a whisker pad and a trigeminal ganglion explant to form the Merkel cell-neurite complex in vitro. We initially developed two distinct culture methods of a single whisker row and a trigeminal ganglion explant, and then combined them. By dissecting and cultivating a single row from a whisker pad, the morphogenesis of whisker follicles could be observed under a microscope. After the co-cultivation of the whisker row with a trigeminal ganglion explant, a Merkel cell-neurite complex composed of Merkel cells, which were positive for both cytokeratin 8 and SOX2, Neurofilament-H-positive trigeminal nerve fibers and Schwann cells expressing Nestin, SOX2 and SOX10 was observed via immunohistochemical analyses. These results suggest that the process for the formation of a Merkel cell-neurite complex can be observed under a microscope using our organotypic co-culture method.

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Acrylamide alters neurotransmitter induced calcium responses in murine ESC-derived and primary neurons

Cited by 32 publications

References 61 publications

Dietary acrylamide exposure was associated with mild cognition decline among non-smoking Chinese elderly men

Dietary acrylamide exposure was associated with mild cognition decline among non-smoking Chinese elderly men

Definition of transcriptome-based indices for quantitative characterization of chemically disturbed stem cell development: introduction of the STOP-Toxukn and STOP-Toxukk tests

In vitro formation of the Merkel cell‐neurite complex in embryonic mouse whiskers using organotypic co‐cultures

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