Acrylamide gel electrophoresis of acid glycosaminoglycans for the study of molecular size distribution

Detection of submicrogram quantities of glycosaminoglycans on agarose gels by sequential staining with toluidine blue and Stains‐All

“…Besides, densitometric analysis of bands permits us to obtain quantitative evaluation of single polysaccharide species in mixtures [3]. Due to these abilities, agarose gel electrophoresis gives us more detailed informations on the complex polysaccharides when used in combination with other analytical approaches, such as polyacrylamide gel electrophoresis that permits the analysis of GAG oligosaccharides [8,9] and also of molecular size distribution [10,11] for single species of polysaccharides, dot-blot assay for quantitative purposes [12], high-performance size-exclusion chromatography for the determination of the polyanions molecular mass and polydispersity [13], and capillary electrophoresis [14].…”

Disaccharide Analysis and Molecular Mass Determination to Microgram Level of Single Sulfated Glycosaminoglycan Species in Mixtures Following Agarose-Gel Electrophoresis

“…Cellulose acetate (12), nitrocellulose membrane (13), agarose-gel (14,15), and polyacrylamide-gel (16,17) electrophoresis are generally utilized for qualitative and quantitative analyses of mixtures of GAGs or single species. Polyacrylamide-gel electrophoresis permits the analysis of GAG oligosaccharides (16,17) and also of molecular size distribution (18,19) for single species of polysaccharides, but it is unable to separate mixtures of different species of polyanions. In contrast, agarose-gel electrophoresis permits the separation and identification of several GAG species, such as slow-and fast-moving HE or HS, DS, and CS (14,15).…”

Electrophoretic approaches to the analysis of complex polysaccharides

“…3B). Thanks to these functions, agarose-gel electrophoresis gives us more detailed information on the complex polysaccharides when used in combination with other analytical approaches, such as polyacrylamidegel electrophoresis for the analysis of GAG oligosaccharides [44,45] and also of molecular size distribution [46,47] for single species of polysaccharides (see below), dot-blot assay for quantitative purposes [48], high-performance size-exclusion chromatography for the determination of polyanion molecular mass and polydispersity [49], and capillary electrophoresis [26] (see below).…”

“…For the first time, Hilborn and Anastassiadis [64] reported the possibility to determine the molecular weight of GAGs by PAGE, and Hsu et al [65] their molecular size distribution. Cowman et al [66] used PAGE to evaluate GAGs oligosaccharides differing in chain length by one disaccharide unit.…”