Acrylic Microspheres In Vivo VII: Morphological Studies on Mice and Cultured Macrophages

Ultrastructural Alterations in Macrophages after Phagocytosis of Acrylic Microspheres

“…In a previous study (3), it was shown that an intravenous injection of 1 mg of particles to mice (weighing -20 g) produced only insignificant changes in the liver, spleen, and bone marrow. This means, that the ratio between the number of particles and cells is -4000 when the cellular alterations can first be detected.…”

“…Preparation of Microparticles-Microparticles with immobilized cationized ferritin were prepared using a reported method (1,3). The cationized ferritin (104 mg), acrylamide (0.3 g), and N,N'-methylenebisacrylamide (0.1 g) were dissolved in 5 mL of 0.005 M sodium phosphate buffer, pH 7.4, while maintaining a nitrogen atmosphere.…”

Blood Clearance and Organ Distribution of Intravenously Administered Polymethacrylic Nanoparticles in Mice

“…These nanospheres are more stable than other types of carriers for which particle clusters have been reported to be mechanically trapped in the lung capillaries. 35. 36 Blood Clearance of Polymethacrylic NanospheresBlood clearance of 1251-labeled nanoparticles was found to be very rapid with a half-life of -3-5 min (Figure 2).…”

Acrylic Microspheres In Vivo VIII: Distribution and Elimination of Polyacryldextran Particles in Mice

“…(0.5 g), was flushed with nitrogen. After addition of the catalyst, ammonium peroxodisulfate (100 pl of a 0.5-g/ml solution), the Eastman Kcdak Co. 2 Sigma Chemical Co. 3 Dextrans (T4O and T500) were obtained from Pharmacia Fine Chemicals, Up sala, Sweden.…”