ACTA2‐AS1 suppresses lung adenocarcinoma progression via sequestering miR‐378a‐3p and miR‐4428 to elevate SOX7 expression

Ying, Kangtai; Wang, Ling; Long, Guangyan; Lian, Chan; Chen, Zhe; Lin, Wei

doi:10.1002/cbin.11451

Cited by 20 publications

(17 citation statements)

References 31 publications

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“…Most of them have been reported that were associated with other cancers and diseases. Silencing of hsa-miR-4428 weaken the proliferative and migratory abilities of lung adenocarcinoma cells [29]. Hsa-miR-575 targeted BLID to promote growth and invasion of non-small cell lung cancer cells [30], moreover hsa-miR-575 exerted oncogenic function in hepatocellular carcinoma by increasing hepatocellular carcinoma proliferation and metastasis [31].…”

Section: Discussionmentioning

confidence: 99%

Downregulation of Hsa-miR-3616-3p in Triple-Negative Breast Cancer is Associated with Cell Migration and Invasion

Peng

Zhang

et al. 2021

Preprint

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Background: Micro RNA (miRNA), a class of endogenous small RNAs with a length of about 20-24 nucleotides, have been played a variety of important regulatory roles in cells and have attracted the attention of researchers because involvement of initiation and progression of diverse kinds of human diseases, especially cancer. However, the regulatory role of miRNA in triple negative breast cancer (TNBC), its clinical significance and potential mechanism are still largely unknown.Methods: In this study, the differentially expressed miRNAs were analyzed using GEO2R from GSE57897 and GSE97811 of the Gene Expression Omnibus (GEO). Then, we performed the overall survival (OS) analysis of four candidate miRNAs. And the expressions of four candidate miRNAs in TNBC and non-TNBC tissues were tested by Quantitative real-time PCR. We determined the level and prognostic values of hsa-miR-3616-3p in TNBC patients. Then, TNBC cells migration and invasion were studied in vitro with hsa-miR-3616-3p by using wounding heal assays and transwell assays. Next, target genes and transcription factors of hsa-miR-3616-3p enrichment analysis, were performed by the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway.Results: Based on GSE57897 and GSE97811, we found 962 differentially expressed miRNAs including 943 up-regulated miRNAs and 19 down-regulated miRNAs. We selected top2 up-regulated miRNAs as hsa-miR-4428 and hsa-miR-575, and top2 down-regulated miRNAs as hsa-miR-3616-3p and hsa-miR-3909, and then we found that low expression of 4 candidated miRNAs had a worse prognosis in TNBC subtype. Next, we verified that the expression of hsa-miR-3616-3p was the most downregulated in TNBC tissues compared with matched surrounding tissues by qRT-PCR. Moerover, our results showed that overexpression of hsa-miR-3616-3p inhibited TNBC cells migration, and invasion in vitro.Conclusions: Our study might reveal important roles of hsa-miR-3616-3p plays in TNBC migration and invasion.

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Section: Discussionmentioning

confidence: 99%

Downregulation of Hsa-miR-3616-3p in Triple-Negative Breast Cancer is Associated with Cell Migration and Invasion

Peng

Zhang

et al. 2021

Preprint

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“… 45 It has been reported that lncRNA actin 2, smooth muscle antisense RNA1 (ACTA2-AS1) plays an anticancer role in liver cancer 46 and inhibits the progression of lung adenocarcinoma by blocking the expression of miR-378A-3P and miR-4428. 47 Several molecular mechanisms underlying the oncogenic role of ACTA2-AS1 have been proposed. ACTA2-AS1 is a well-known lncRNA associated with lung adenocarcinoma, 47 cervical cancer, 48 ovarian cancer, BRCA, 49 and liver cancer.…”

Section: Discussionmentioning

confidence: 99%

“…47 Several molecular mechanisms underlying the oncogenic role of ACTA2-AS1 have been proposed. ACTA2-AS1 is a well-known lncRNA associated with lung adenocarcinoma, 47 cervical cancer, 48 ovarian cancer, BRCA, 49 and liver cancer. 46 In summary, our study proves that HR-lncRNAs are associated with poor prognosis and short overall survival.…”

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confidence: 99%

Construction and Verification of a Hypoxia-Related 4-lncRNA Model for Prediction of Breast Cancer

Zhao¹,

Liu²,

Zhao³

et al. 2021

IJGM

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Introduction Breast cancer is the most common form of cancer worldwide and a serious threat to women. Hypoxia is thought to be associated with poor prognosis of patients with cancer. Long non-coding RNAs are differentially expressed during tumorigenesis and can serve as unambiguous molecular biomarkers for the prognosis of breast cancer. Methods Here, we accessed the data from The Cancer Genome Atlas for model construction and performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses to identify biological functions. Four prognostic hypoxia-related lncRNAs identified by univariate, LASSO, and multivariate Cox regression analyses were used to develop a prognostic risk-related signature. Kaplan–Meier and receiver operating characteristic curve analyses were performed, and independent prognostic factor analysis and correlation analysis with clinical characteristics were utilized to evaluate the specificity and sensitivity of the signature. Survival analysis and receiver operating characteristic curve analyses of the validation cohort were operated to corroborate the robustness of the model. Results Our results demonstrate the development of a reliable prognostic gene signature comprising four long non-coding RNAs (AL031316.1, AC004585.1, LINC01235, and ACTA2-AS1). The signature displayed irreplaceable prognostic power for overall survival in patients with breast cancer in both the training and validation cohorts. Furthermore, immune cell infiltration analysis revealed that B cells, CD4 T cells, CD8 T cells, neutrophils, and dendritic cells were significantly different between the high-risk and low-risk groups. The high-risk and low-risk groups could be precisely distinguished using the risk signature to predict patient outcomes. Discussion In summary, our study proves that hypoxia-related long non-coding RNAs serve as accurate indicators of poor prognosis and short overall survival, and are likely to act as potential targets for future cancer therapy.

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“…Studies have indicated that SOX7 is a tumor suppressor gene, down-regulated in most tumors. The regulatory mechanisms may be through regulating the transcription process mediated by Wnt-β-catenin signaling pathway, inhibiting tumor proliferation, migration, and invasion [36][37][38]. Given all this, these upstream transcription factors played central roles in the improvement of psoriasis following TDGs.…”

Section: Discussionmentioning

confidence: 99%

Gene Set Enrichment Analysis and Ingenuity Pathway Analysis to verify the impact on Wnt Signaling Pathway in Taodan Granules Treated Psoriasis

Luo¹,

Zhang²,

Su³

et al. 2021

Preprint

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Background: Taodan granules (TDGs), traditional Chinese herbals, are effective in treating psoriasis. However, mechanisms of TDGs remain indistinct. The current study aims to indicate the molecular mechanisms of TDGs in treating psoriasis.Methods: Primarily, transcriptional profiling was applied to identify differentially expression genes (DEGs). The following was that we used Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) analysis for functional enrichment analysis. Eventually, RT-PCR was performed to validation. Results: The results revealed that TDGs could regulated the Wnt signaling pathway to ameliorate skin lesions of imiquimod (IMQ)-induced psoriatic mouse models. IPA core network associated with Wnt signaling pathways in TDGs for psoriasis was established. Thereinto zeste homolog (EZH) 2, CTNNB1, TP63, and WD repeat domain (WDR) 5 could be considered as upstream genes in the Wnt signaling pathway.Conclusions: The Wnt signaling pathway with these above upstream genes might be potential therapeutic targets of TDGs for psoriasis.

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ACTA2‐AS1 suppresses lung adenocarcinoma progression via sequestering miR‐378a‐3p and miR‐4428 to elevate SOX7 expression

Cited by 20 publications

References 31 publications

Downregulation of Hsa-miR-3616-3p in Triple-Negative Breast Cancer is Associated with Cell Migration and Invasion

Downregulation of Hsa-miR-3616-3p in Triple-Negative Breast Cancer is Associated with Cell Migration and Invasion

Construction and Verification of a Hypoxia-Related 4-lncRNA Model for Prediction of Breast Cancer

Gene Set Enrichment Analysis and Ingenuity Pathway Analysis to verify the impact on Wnt Signaling Pathway in Taodan Granules Treated Psoriasis

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