Acteoside Protects Retinal Ganglion Cells from Experimental Glaucoma by Activating PI3K/AKT Signaling Pathway via Upregulating Caveolin 1

Xi, Xiaoting; Chen, Qianbi; Ma, Jia; Wang, Xuewei; Yuan, Xianglin; Wen, Xuewwei; Cai, Bin; Li, Yan

doi:10.2139/ssrn.3925505

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Yuan et al [51] found that acteoside and caspase-3 played a neuroprotective role in PD rat model. Xi et al [52] have found that acteoside can attenuate RGC loss and oxidative stress by activating PI3K/AKT signaling in the ocular hypertension (HP) rat model. In vitro cell data suggested that acteoside had a significant neuroprotective effect on glutamate-induced neurotoxicity in primary cultured rat cortical cells [27] .…”

Section: Acteoside Relieves Cell Injury In Glutamate-induced Rgc5 Cel...mentioning

confidence: 99%

Neuroprotective effects of acteoside in a glaucoma mouse model by targeting Serta domain-containing protein 4

Hao,

Yu,

Liu

et al. 2024

Int J Ophthalmol

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AIM: To explore the therapeutic effect and main molecular mechanisms of acteoside in a glaucoma model in DBA/2J mice. METHODS: Proteomics was used to compare the differentially expressed proteins of C57 and DBA/2J mice. After acteoside administration in DBA/2J mice, anterior segment observation, intraocular pressure (IOP) monitoring, electrophysiology examination, and hematoxylin and eosin staining were used to analyze any potential effects. Immunohistochemistry (IHC) assays were used to verify the proteomics results. Furthermore, retinal ganglion cell 5 (RGC5) cell proliferation was assessed with cell counting kit-8 (CCK-8) assays. Serta domain-containing protein 4 (Sertad4) mRNA and protein expression levels were measured by qRT-PCR and Western blot analysis, respectively. RESULTS: Proteomics analysis suggested that Sertad4 was the most significantly differentially expressed protein. Compared with the saline group, the acteoside treatment group showed decreased IOP, improved N1-P1 wave amplitudes, thicker retina, and larger numbers of cells in the ganglion cell layer (GCL). The IHC results showed that Sertad4 expression levels in DBA/2J mice treated with acteoside were significantly lower than in the saline group. Acteoside treatment could improve RGC5 cell survival and reduce the Sertad4 mRNA and protein expression levels after glutamate injury. CONCLUSION: Sertad4 is differentially expressed in DBA/2J mice. Acteoside can protect RGCs from damage, possibly through the downregulation of Sertad4, and has a potential use in glaucoma treatment.

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Section: Acteoside Relieves Cell Injury In Glutamate-induced Rgc5 Cel...mentioning

confidence: 99%

Neuroprotective effects of acteoside in a glaucoma mouse model by targeting Serta domain-containing protein 4

Hao,

Yu,

Liu

et al. 2024

Int J Ophthalmol

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Anti-Candidal Activity of the Parasitic Plant Orobanche crenata Forssk

et al. 2021

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Candida albicans (C. albicans) and Candida glabrata (C. glabrata) are part of the human microbiome. However, they possess numerous virulence factors, which confer them the ability to cause both local and systemic infections. Candidiasis can involve multiple organs, including the eye. In the present study, we investigated the anti-candidal activity and the re-epithelizing effect of Orobanche crenata leaf extract (OCLE). By the microdilution method, we demonstrated an inhibitory effect of OCLE on both C. albicans and C. glabrata growth. By crystal violet and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we showed the ability of OCLE to inhibit the biofilm formation and the viability of yeast cells, respectively. By germ tube and adhesion assays, we proved the capacity of OCLE to affect the morphological transition of C. albicans and the adhesion of both pathogens to human retinal pigment epithelial cells (ARPE-19), respectively. Besides, by MTT and wound healing assay, we evaluated the cytotoxic and re-epithelizing effects of OCLE on ARPE-19. Finally, the Folin–Ciocalteu and the ultra-performance liquid chromatography-tandem mass spectrometry revealed a high content of phenols and the presence of several bioactive molecules in the extract. Our results highlighted new properties of O. crenata, useful in the control of Candida infections.

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Acteoside Protects Retinal Ganglion Cells from Experimental Glaucoma by Activating PI3K/AKT Signaling Pathway via Upregulating Caveolin 1

Cited by 2 publications

References 30 publications

Neuroprotective effects of acteoside in a glaucoma mouse model by targeting Serta domain-containing protein 4

Neuroprotective effects of acteoside in a glaucoma mouse model by targeting Serta domain-containing protein 4

Anti-Candidal Activity of the Parasitic Plant Orobanche crenata Forssk

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