ACTH antibodies and their use for a radio-immunoassay for ACTH

Application of the Coated Charcoal Separation Method to the Radioimmunoassay of Plasma Corticotrophin

“…These advantages also apply to the silicate separation procedure of Rosselin, Assan, Yalow & Berson (1966) and the use of ion exchange resins (Melani, 1966), although the latter are more difficult to prepare and separate. The double antibody technique is less rapid, is subject to additional errors due to the presence of a second antibody (Brunfeldt & J0rgensen, 1967) and has been associated with ACTH values that are con¬ siderably higher than those reported by other workers (Felber, 1963). Using chromatoelectrophoresis (Berson et al 1956), it is possible to distinguish between labelled hormone fragments and 131Iodide and this makes the method especially suitable for assessing iodination yields.…”

The experimental generation of antibodies to alpha-melanocyte stimulating hormone and adrenocorticotropic hormone.

“…In the case of a-MSH, specific antibodies were rapidly induced by the hormone-RSA conjugate. Unconjugated ACTH, on the other hand, can induce antibodies when injected into rabbits or guinea pigs (6,7,12,13). Antibodies formed in response to unconjugated ACTH are more specific than those produced in the current study.…”

“…tide hormones, including insulin (2), glucagon (3), parathormone (4,5), and ACTH (6,7). These techniques depend on the availability of antibodies to the hormones.…”

Non-Specific Effects of Synthetic Corticotrophin Polypeptides

“…Likewise, H ofmann et al (1) have not succeeded in producing antibody to synthetic /^-^-corticotrophin whereas antibody to the protected polypeptide was obtained with relative ease. There is also evidence, however, that under certain conditions antibody to synthetic polypeptides of the size used in this study can be elicited experimentally: Using a radioimmunc assay (complex formation between radio-iodine labelled ACTH and antiserum), Felber (5) has demonstrated antibody to pure porcine cortico trophin. And G oodfriend el al.…”