ACTH Antibodies in Patients Receiving Depot Porcine ACTH to Hasten Recovery from Pituitary-Adrenal Suppression*

Demonstration of Circulating Antibodies to Neurophysin in Patients Treated With Pitressin

“…In one patient (F) the anti¬ body titre exceeded that found in rabbits after immunization with bovine neuro¬ physin ; it should be noted, however, that at this stage the rabbits had been immunized for only 6 months. The antibody titres found in these patients were much higher than those produced in man in response to treatment with smaller peptide hormones such as corticotrophin (Fleischer, Abe, Liddle, Orth & Nicholson, 1967;Landon, Friedman & Greenwood, 1967) and insulin (Berson & Yalow, 1959). Production of antibodies to neurophysin is not surprising since we found that aqueous Pitressin contains neurophysin at a concentration of approximately 10 µg|m.í, and the material is given in oil as a depot preparation.…”

An evaluation of antibodies and clinical resistance to salmon calcitonin

“…The unique features seen in this patient included: (a) the development of resistance to the biological effects of salmon calcitonin with the loss of the hypocalcemic response to the hormone and a return of indices of skeletal turnover to pretreatment values despite continued treatment; (b) a far greater antibody titer (maximum 1: 30,000) than was found in any other patient; and (c) inactivation by his plasma of salmon calcitonin in the rat bioassay. There was no clinical evi- (24,25), and human growth hormone (26). Almost all diabetics treated with bovine and porcine insulin develop antibodies to insulin, but only a few are insulin resistant.…”

“…Resistance to vasopressin has not been documented and only one patient with antibodies and resistance to bovine PTH has been described (23). Antibodies to porcine ACTH given in gelatin and as a zinc phosphate complex commonly result in antibody formation, but resistance to hormone action has not been proven (24,25). In these reports the foreign protein nature of the hormones has been used as the explanation for the development of antibodies.…”

Development of antibodies during long-term therapy with corticotrophin in rheumatoid arthritis. I. Porcine ACTH.

“…Corticotrophin (ACTH), a straight-chain polypeptide of 39 amino acids, is a relatively poor antigen; nevertheless antibodies to it have been reported (Fleischer, Abe, Liddle, Orth, and Nicholson, 1967;Landon, Friedman, and Greenwood, 1967). Imura, Sparks, Grodsky, and Forsham (1965) have demonstrated in rabbits that the antibodycombining site on the ACTH molecule is towards the 'C' terminal end, away from the biologically active 'N' terminal sequence of the molecule (Fig.…”