Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells

Rudnicki, Michael A.; Jackowski, George; Saggin, Leopoldo; McBurney, Michael W.

doi:10.1016/0012-1606(90)90202-t

Cited by 106 publications

(54 citation statements)

References 27 publications

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“…Development of lateral muscle fibres and myosin isoforms was studied on transverse body sections and through a "cranial-caudal" examination as well as at different ages. Five antibodies specific for various isoforms of myosin were used: (1) 3-96: polyclonal anti-fish (Sparus aurata, Linnaeus) fast myosin; (2) 4-96: polyclonal anti-fish (Sparus aurata) slow myosin (Veggetti et al, 1999;Stoiber et al, 2002); (3) BA-G5: monoclonal anti-mammalian (Rattus norvegicus, Berkenhout) cardiac alpha myosin (Rudnicki et al, 1990); (4) SC-71: monoclonal anti-mammalian (Rattus norvegicus) type 2A myosin (Schiaffino et al, 1989) and (5) BA-F8: monoclonal anti-mammalian (Rattus norvegicus) slow beta myosin (Borrione et al, 1988). The polyclonal antibodies were raised by AMR and colleagues, and the SC-71, BA-F8 and BA-G5 monoclonals were obtained from hybridomas originally produced by Prof. S. Schiaffino and colleagues.…”

Section: Methodsmentioning

confidence: 99%

Muscle differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): Histochemical and immunohistochemical study of the fibre types

Silva¹,

Rowlerson²,

Valente³

et al. 2008

Tissue and Cell

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The aim of this work was to gain insights into the mechanism of muscle differentiation and growth in Pagellus bogaraveo, by studying muscle fibre phenotypes identified by immunohistochemistry. At hatching, several layers of deep fast-white fibres were covered by a superficial fibre monolayer. At 5 days, slow-red fibres appeared near the lateral line nerve. At 40 days, the intermediate-pink muscle became visible, and in the slow-red and fast-white muscle layers transitions from larval myosin isoforms to the isoforms typical of adult muscle occurred. Between 70 and 100 days, small fibres with a distinct ATPase profile appeared throughout the fast-white muscle, marking the onset of "mosaic" hyperplasia. The myosin of the original superficial monolayer fibres underwent two myosin transformations, before being slowly replaced by an adult slow-red isoform. In juveniles and adults, the slow-red muscle layer could be resolved into two distinct types. The analysis of fibre phenotypes indicated that post-larval muscle growth occurred by two distinct stages of hyperplasia. This study offers a basis for further comparative and experimental studies with this economically relevant species, namely for identifying factors influencing its muscle growth dynamics and disclosing underlying mechanisms.

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Section: Methodsmentioning

confidence: 99%

Muscle differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): Histochemical and immunohistochemical study of the fibre types

Silva¹,

Rowlerson²,

Valente³

et al. 2008

Tissue and Cell

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“…Egr-1 expression is rapidly and transiently induced during the transition of cells from the G 0 to G 1 phase in response to various mitogens such as growth factors, cytokines, injury, and partial hepatectomy. (7,9,(13)(14)(15)(16)(17)(18)(19) In this study, we demonstrated that the expression of PRL-1 gene was dramatically induced at a transcriptional level in the remnant liver following partial hepatectomy. Egr-1 protein expression and Egr-1 binding activity increased in concert with PRL-1 gene transcription in regenerating liver.…”

mentioning

confidence: 87%

Mitogenic Up-regulation of the PRL-1 Protein-tyrosine Phosphatase Gene by Egr-1

Peng¹,

Du²,

Ramirez³

et al. 1999

Journal of Biological Chemistry

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The cellular signals that initiate cell growth are incompletely understood. Insight could be provided by understanding the signals regulating the transcriptional induction of immediate-early genes which occurs within minutes of the growth stimulus. The expression of the PRL-1 gene, which encodes a unique nuclear protein-tyrosine phosphatase, is rapidly induced in regenerating liver and mitogen-treated cells. Transcription of the PRL-1 gene increased in the rat liver remnant within a few minutes after partial hepatectomy and largely explained the increase in steady-state PRL-1 mRNA in the first few hours posthepatectomy. Egr-1 (early growth response factor) specifically bound a region of the proximal PRL-1 promoter P1 (؊99). Egr-1 binding activity was more rapidly induced in regenerating liver than mitogen-treated H35 and NIH 3T3 cells, remained elevated through 4 h posthepatectomy, and appeared to be dependent not only on new Egr-1 protein synthesis but on post-translational regulation of Egr-1. Egr-1 efficiently transactivated a PRL-1 promoter reporter construct containing an intact not mutant Egr-1 site, and the Egr-1 site largely accounted for PRL-1 gene up-regulation in response to mitogen stimulation. These data predict that Egr-1 activation is an early event in liver regeneration and mitogen-activated cells that provides a regulatory stimulus for a subset of immediateearly genes.

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“…The sections were subsequently incubated at room temperature in the appropriate dilution of specific monoclonal antibodies raised against ~ (BA-G5), IIa (SC-71), IIb (BF-F3), IIx and IIb (212F), Emb (F1.652), Emb, neonatal, IIx and IIb (RT-D9), against all MHC except type IIx (BF-35), and developmentally regulated (A4.840, A4.951 and N2.261) and adult (BA-D5, BA-F8) epitopes of I MHC [2,[31][32][33]. The sections were then rinsed with PBS (3 x 10 min) and incubated for 2 h at room temperature in peroxidase-labelled goat anti-mouse IgG, or IgM in the case of BF-F3 and A4.840 (Sigma, St. Louis, MO).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short‐term paralysis: comparison of denervation and tetrodotoxin‐induced neural inactivation

1996

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The extent to which myosin profiles within aduR fast and slow muscles are altered by short-term paralysis remains equivocal. We used an array of specific antibodies to identify adult and developmental MHC isoforms within EDL and soleus muscle fibers, and show a marked multiple expression of MHCs with a general shift towards slower and more energy efficient MHC profiles after 2 weeks of denervation or TTX nerve conduction block. Paralysis also induced marked expression of an embryonic MHC within most EDL cell types, and a subtle, paralysis-sensitive, expression of ¢x-cardiac MHC within speeific EDL and soleus extrafusal fibers. Comparison of treatment groups also permitted assessment of the relative influence of neural activity versus trophic factors on these isoforms, and confirmed activity as a major, but not sole, regulator of MHC expression.

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Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells

Cited by 106 publications

References 27 publications

Muscle differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): Histochemical and immunohistochemical study of the fibre types

Muscle differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): Histochemical and immunohistochemical study of the fibre types

Mitogenic Up-regulation of the PRL-1 Protein-tyrosine Phosphatase Gene by Egr-1

Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short‐term paralysis: comparison of denervation and tetrodotoxin‐induced neural inactivation

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